Immunology & Inflammation related

Repression of BSEP gene expression upon treatment with reported BSEP inhibitors in human primary hepatocytes. BSEP mRNA expression was measured using real-time PCR in hepatocytes from at least three donors upon treatment with BSEP inhibitors as described under Materials and Methods. All the data are expressed as mean ?S.D. (n = 3).

Pim inhibits nuclear factor of activated T-cell (NFAT) translocation of cultured human vascular smooth muscle cells. hCASM expressing green fluorescent protein-NFAT were incubated with 10 uM Pim for 30 min. Left: mean nuclear/cytosol fluorescence intensity ratio (±SE) is displayed at basal conditions and after stimulation with 500 uM ATP in the absence and presence of Pim.

The aminopeptidase-N inhibitor, bestatin (C), showed an inhibitory effect on cell proliferation that predominated in mixed-IHCCA. *p< 0.05 mucin vs mixed, N experiments = 5-7.

Differential participation of ATRs or MCP-1/CCR2 signaling in RVLM in pressor response and tachycardia after stroke. Changes in mean diastolic (ΔDBP) blood pressure or heart rate (ΔHR) relative to baseline in rats that received pretreatment by microinjection bilaterally into the RVLM of bindarit (Bin; 10 nmol, MCP-1 synthesis inhibitor), BMS CCR2 22 (BMS; 10 pmol, CCR2 antagonist), propagermanium (Prop; 10 pmol, CCR2 antagonist) or vehicle control (Veh).Values are mean ± SEM, n = 5-7 animals per experimental group. *P < 0.05 versus SC group, and +P < 0.05 versus Veh (Saline) + MCAO group in the post hoc Scheffe multiple-range test.

(B) Florescent images of EpEX (green) and EpICD (red) in PK-15 cells, which were transfected by pSIN-fEpCAM-mCherry and treated with or without TAPI-1/DAPT. Nuclei were stained by Hoechst 33342 (blue). Scale bar, 25 μm.

Combinations of EPZ-6438 with Prednisolone or Dexamethasone in DOHH2 EZH2 wild-type GCB lymphoma cell lines. All dose response plots were generated in Graphpad Prism and curves fitted to a four-parameter model with variable slope (2 biological replicates). Doses of EPZ-6438 ranged from 15.6-1000 nM, doses of Prednisolone ranged from 7.8–1000 nM, and doses of Dexamethasone ranged from
0.8-100 nM. The potency of Prednisolone is increased with addition of EPZ-6438 in DOHH2 cells.

Motor disturbances of the rat hindlimbs after SCI, using an inclined plane test. Key: ◇ = PBS group; ■ = Sal B group; ▲ = methylprednisolone group.

Cells were cultured in LD collagen gels ± vehicle control (V), 100 M DFOM (D) or tranilast (T) for 24h (E-H) and assessed for P450 enzyme production.

Leflunomide or teriflunomide were incubated with H1299, H460, A549 cells at indicated concentrations for or 2, 4 or 6 days, followed by MTT assay.

Repression of BSEP gene expression upon treatment with reported BSEP inhibitors in human primary hepatocytes. BSEP mRNA expression was measured using real-time PCR in hepatocytes from at least three donors upon treatment with BSEP inhibitors as described under Materials and Methods. All the data are expressed as mean ?S.D. (n = 3).

Pim inhibits nuclear factor of activated T-cell (NFAT) translocation of cultured human vascular smooth muscle cells. hCASM expressing green fluorescent protein-NFAT were incubated with 10 uM Pim for 30 min. Left: mean nuclear/cytosol fluorescence intensity ratio (±SE) is displayed at basal conditions and after stimulation with 500 uM ATP in the absence and presence of Pim.

The aminopeptidase-N inhibitor, bestatin (C), showed an inhibitory effect on cell proliferation that predominated in mixed-IHCCA. *p< 0.05 mucin vs mixed, N experiments = 5-7.

Differential participation of ATRs or MCP-1/CCR2 signaling in RVLM in pressor response and tachycardia after stroke. Changes in mean diastolic (ΔDBP) blood pressure or heart rate (ΔHR) relative to baseline in rats that received pretreatment by microinjection bilaterally into the RVLM of bindarit (Bin; 10 nmol, MCP-1 synthesis inhibitor), BMS CCR2 22 (BMS; 10 pmol, CCR2 antagonist), propagermanium (Prop; 10 pmol, CCR2 antagonist) or vehicle control (Veh).Values are mean ± SEM, n = 5-7 animals per experimental group. *P < 0.05 versus SC group, and +P < 0.05 versus Veh (Saline) + MCAO group in the post hoc Scheffe multiple-range test.

(B) Florescent images of EpEX (green) and EpICD (red) in PK-15 cells, which were transfected by pSIN-fEpCAM-mCherry and treated with or without TAPI-1/DAPT. Nuclei were stained by Hoechst 33342 (blue). Scale bar, 25 μm.

Combinations of EPZ-6438 with Prednisolone or Dexamethasone in DOHH2 EZH2 wild-type GCB lymphoma cell lines. All dose response plots were generated in Graphpad Prism and curves fitted to a four-parameter model with variable slope (2 biological replicates). Doses of EPZ-6438 ranged from 15.6-1000 nM, doses of Prednisolone ranged from 7.8–1000 nM, and doses of Dexamethasone ranged from
0.8-100 nM. The potency of Prednisolone is increased with addition of EPZ-6438 in DOHH2 cells.

Motor disturbances of the rat hindlimbs after SCI, using an inclined plane test. Key: ◇ = PBS group; ■ = Sal B group; ▲ = methylprednisolone group.

Cells were cultured in LD collagen gels ± vehicle control (V), 100 M DFOM (D) or tranilast (T) for 24h (E-H) and assessed for P450 enzyme production.

Leflunomide or teriflunomide were incubated with H1299, H460, A549 cells at indicated concentrations for or 2, 4 or 6 days, followed by MTT assay.