Scopoletin

Catalog No.S3881 Synonyms: Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid

For research use only.

Scopoletin (Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid) is the main bioactive constituent of flower buds from Magnolia fargesii with anti-inflammatory, hypouricemic, and antioxidant activities.

Scopoletin Chemical Structure

CAS No. 92-61-5

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Biological Activity

Description Scopoletin (Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid) is the main bioactive constituent of flower buds from Magnolia fargesii with anti-inflammatory, hypouricemic, and antioxidant activities.
In vitro

Scopoletin (1-50 μg/ml) inhibits the release of PGE2, TNF-a, IL-1b and IL-6 and suppresses the expression of COX-2 in a concentration-dependent manner[1]. In vitro studies reveal that scopoletin lessens rotenone-induced apoptosis in SH-SY5Y cells through preventing oxidative injury. Particularly, scopoletin markedly up-regulates DJ-1, which then promotes the nuclear translocation of Nrf2 and transactivation of antioxidant genes. Scopoletin prevents the nuclear exportation of Nrf2 by reducing the levels of Keap1 and thereby enhancing the neuronal defence system[3].

In vivo Scopoletin expressively represses the aortic oxido-lipidemic stress and thereby upholding normal morphology of the aorta, and thus minimizing the peril of CVD[2].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: RAW 264.7 cell line
  • Concentrations: 1-50 μg/ml
  • Incubation Time: 2 h
  • Method: Cells (1×106/ml) are pre-incubated 2 h with scopoletin (1-50 μg/ml) and further cultured 6 h or 12 h with LPS (100 ngyml) in 24-well plates. Supernatants are removed at the allotted times and PGE2, TNF-a, IL-1b and IL-6 levels are quantified by immunoassay kits.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Healthy male albino rats of Wistar strain
  • Dosages: 10 mg/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 38 mg/mL
(197.74 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 192.17
Formula

C10H8O4

CAS No. 92-61-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C=C2C(=C1)C=CC(=O)O2)O

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