2x SYBR Green qPCR Master Mix (Low ROX)

For research use only.

2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR.

2x SYBR Green qPCR Master Mix (Low ROX)

Advantages

High Sensitivity: Lower Ct values ensure higher efficiency of amplification

Cost Effective: Offered at much discounted price compared to market price

Price Comparison

Description

2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with low concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 7500, 7500 Fast, ViiATM7; Stratagene MX4000TM, MX3005PTM, MX3000PTM.

Applications

  • Dye-based quantitative PCR detection
  • Nucleic acid amplification and expression profiling
  • Quantitative genotyping studies
  • Genomics-related applications

Components

Component Cat#: B21702 Cat#: B21703
2x SYBR Green qPCR Master Mix(Low ROX) * 5 mL
(for 200 reactions with 50 μl/rxn
for 500 reactions with 20 μl/rxn
for 1000 reactions with 10 μl/rxn)
25 mL
(for 1000 reactions with 50 μl/rxn
for 2500 reactions with 20 μl/rxn
for 5000 reactions with 10 μl/rxn)
* Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye and ROX Dye (Low concentration)

Storage

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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