Cyclosporin A (NSC 290193)

Catalog No.S2286 Synonyms: Cyclosporine A, Cyclosporine, Ciclosporin, CsA

For research use only.

Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Selleck's Cyclosporin A (NSC 290193) has been cited by 64 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.
Targets
calcineurin [1]
(Cell-free assay)
7 nM
In vitro

Cyclosporin A binds to the cyclophilin (immunophilin) in T cells[1] , forms a Cyclophilin-Cyclosporin A complex which the binds to and inhibits calcineurin. [2] Cyclosporin A inhibits calcineurin with IC50 of 7 nM[3], then blocks the nuclear translocation of NF-AT. [4] Cyclosporin A also affects mitochondria by preventing the MTP (mitochondrial permeability transition pore) from opening with an IC50 of 39 nM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells NWnaWms{S3m2b4TvfIlkyqCjc4PhfS=> NF3Ie|hEgXSxdH;4bYNqfHliYXfhbY5{fCC{YYSgTFQzTSClZXzsd{Bie3Onc4Pl[EBieyCrboTyZYNmdGy3bHHyJGFVWCCuZY\lcEwhXEN3ME20NkDPxE1? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTVyMkC1PEc,OTl3MEKwOVg9N2F-
mouse L929 cells M1fiRmN6fG:2b4jpZ:Kh[XO|YYm= M2HZSFMh\GG7cx?= M3nRbmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw6OjliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VWyCjc4PhfUwhUUN3ME2zNEDPxE1? NWXk[GtZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFUyQTVpPkK1PVg2OTl3PD;hQi=>
human Huh-Mono cells MYnDfZRwfG:6aXRCpIF{e2G7 NFmwW2E{KGSjeYO= M2PPcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2cC2Pb37vJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XoxvJygR2M2OD1{NDFOwG0> NVfycWMyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
MDCK cells M4S2XmN6fG:2b4jpZ:Kh[XO|YYm= MnKyR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSFSzDj[YxteyxiVFO1NF06NjlizszN MonlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells NUj0[I5xS3m2b4TvfIlkyqCjc4PhfS=> NUL0O5RnPzJiaB?= NFrTRnNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNS0NvNjDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTB;OD6zJO69VQ>? NGL2OnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNFQxOid-MkKzNlA1ODJ:L3G+
human HeLa cells NWrwdZYyS3m2b4TvfIlkyqCjc4PhfS=> NEDuVmJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{NCCWQ{WwQVgvOyEQvF2= M1KxZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells MXTGeY5kfGmxbjDhd5NigQ>? MkH2NlQhcA>? NHf1UmlCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBY\XO2IF7pcIUhfmm{dYOgbY5n\WO2ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC4aYLhcEBTVkFic4nueIhme2m|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;ODFOwG0> NVTlfngyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVEzQDZpPkG5OFUyOjh4PD;hQi=>
human Huh-9-13 cells M{jNSWN6fG:2b4jpZ:Kh[XO|YYm= MWizJIRigXN? NELPVI5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvOT2xN{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;NzFOwG0> NUDLbHQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
human HuH6 cells NWPXNGFVS3m2b4TvfIlkyqCjc4PhfS=> MVGzJIRigXN? NWTTZ2trS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNjDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCFQ{WwQVch|ryP M2\zRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human Huh5-2 cells NX3QToFFS3m2b4TvfIlkyqCjc4PhfS=> M37YcFMh\GG7cx?= MnPvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPS1{IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZkhNCCFQ{WwQVYh|ryP M3nsUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH7 cells NVvkRlk{S3m2b4TvfIlkyqCjc4PhfS=> MmTkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXzlZZNmKG:oIHzhZ5RifGViZHXofYRzd2enbnHz[UwhUUN3ME21MlgyKM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl2M{O5NEc,OjB7NEOzPVA9N2F-
human 2008 cells NV\xTYJwTnWwY4Tpc44h[XO|YYm= M1SxWmlvcGmkaYTpc44hd2ZiaIXtZY4hVVKSMTDpckBpfW2jbjCyNFA5KGOnbHzzMEBKSzVyPUSuO|gh|ryP NHfLXYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEewO|g5PCd-MUi3NFc5QDR:L3G+
human HeLa cells M4TIdmZ2dmO2aX;uJIF{e2G7 M135RmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1zODDpcoZm[3SnZDDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNzDkZZl{97zOIGTDOVA:PC54IN88US=> NWHaOJBSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyNVIyODBpPkG4NlEzOTByPD;hQi=>
leukemia CEM cells M3PZPGZ2dmO2aX;uJIF{e2G7 M2HDZmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDNSHIyKFBvR3z5Z49xem:2ZXnuJGFDSyCWcnHud5BwenSncjD1d4lv\yCuZYXr[Y1q[SCFRV2gZ4VtdHNuIFnDOVA:Oy52IN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjN4MUO4O{c,OTJ|NkGzPFc9N2F-
human Caco2 cells M17lXWZ2dmO2aX;uJIF{e2G7 NGj5WWdvcGmkaYTpc44hd2ZiUD3ndEBqdiCqdX3hckBE[WOxMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\Gmpb4jpckBm\m[udYisJGlEPTB;Mj65JO69VQ>? Mke1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OEK0OVgoRjJ|M{iyOFU5RC:jPh?=
human MDA435/LCC6MDR cells NFraR|NEgXSxdH;4bYPDqGG|c3H5 MXG3Nog> MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGE1OzVxTFPDOm1FWiClZXzsd{BmgHC{ZYPzbY5oKE2GUjDh[pRmeiB5MjDodpPwxIxiSVO1NF0zNjhizszN M{K5[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
guinea pig ventricular myocytes MYTGeY5kfGmxbjDhd5NigQ>? M3yyWmlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1? M1XtU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{[xNFAxLz5{Mke2NVAxODxxYU6=
KB/MDR cell NED0[nZHfW6ldHnvckBie3OjeR?= M3nhcFczKGh? NXvxcJVFUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEL13EVkBk\WyuIHzpcoUh[W[2ZYKgO|IhcHJib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0yNjVizszN MnfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2OEG5PVEoRjF3NEixPVkyRC:jPh?=
A2780/ADR cells MVzGeY5kfGmxbjDhd5NigQ>? NVG2UJNHUW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdiCneIDy[ZN{\WRiaX6gRVI4QDBxQVTSJINmdGy|IHL5JINidGOnaX6gRW0h[XO|YYpvwKwhUUN3ME2xMlQyOjV2IN88US=> M{m4Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEmwNFk1Lz5zN{i5NFA6PDxxYU6=
human K562/R7 cells NV;XVIhxTnWwY4Tpc44h[XO|YYm= M1\VRVEh|ryP NV7ncmk{PzJiaB?= NF;ZRYVRd3SnboTpZZRqd25ib3[g[I95d3K3YnnjbY4ucW6mdXPl[EBkgXSxdH;4bYNqfHliYXfhbY5{fCCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iS{W2Nk9TPyClZXzsd{Bie3Onc4Pl[EBieyCmb4jvdpVjcWOrbjDJR|UxKGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlch|ryP MlLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{SwOFEoRjJ3NkO0NFQyRC:jPh?=
LLC-PK1 epithelial cells MkXwSpVv[3Srb36gZZN{[Xl? MULJcohq[mm2aX;uJI9nKFBvZ3z5Z49xem:2ZXnuMEBud3W|ZTDMMY1lejGkIHX4dJJme3OnZDDpckBNVENvUFuxJIVxcXSqZXzpZYwh[2WubIOgeZNqdmdiY3HsZ4Vqdi2DTTDwc4xiemm|YYTpc44h[XO|YYpvwKwhUUN3ME2wMlch|ryP M4XydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
mouse L5178 cells M1e5VGZ2dmO2aX;uJIF{e2G7 MWOyNEBucW6| MnG4TY5pcWKrdHnvckBw\iCqdX3hckBCSkOEMT3t[YRq[XSnZDDybI9l[W2rbnWgNVI{KGWoZnz1fEBqdiCvb4Xz[UBNPTF5ODDj[YxteyCneIDy[ZN{cW6pIHj1cYFvKE2GUkGgZYZ1\XJiMkCgcYlveyCkeTDGRWNUKGGwYXz5d4l{NCCLQ{WwQVAvPjVizszN M3exT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxO|Q2Lz5{MUO0NVc1PTxxYU6=
KB cell Mo\KSpVv[3Srb36gZZN{[Xl? MWHJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgT2Ih[2WubDDsbY5mKGGodHXyJFczKGhib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0xNjZizszN M3HrNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
mouse mammary carcinoma cell line EMT6/AR 1.0 M{\VVWZ2dmO2aX;uJIF{e2G7 MYDS[ZZmenOjbDDl[oZm[3Rib36geIhmKGGlY4XteYxifGmxbjDv[kBcO0ifLTDkZZVvd3K3YnnjbY4hcW5ibX;1d4UhdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mKEWPVE[vRXIhOS5yLDDJR|UxRTBwNESg{txO NHXLXXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEC5PFY4OSd-MUCwPVg3PzF:L3G+
CK1 cells M2HvR2Z2dmO2aX;uJIF{e2G7 NH3yZpBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJWFZvM{OgbY5n\WO2ZXSgbY4hS0tzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgO{Bl[Xm|LDDUR|UxRTBwMzFOwG0> NVLpT5VGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyNVIyODBpPkG4NlEzOTByPD;hQi=>
HEK293 cells M3n3PWZ2dmO2aX;uJIF{e2G7 Mn3yTY5pcWKrdHnvckBw\iCRQWTQNWIyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCycn;0[YlvNW2nZHnheIVlKHCrdHH2ZZN1[XSrbjD1dJRic2YxvJygT4k:OC5{IN88US=> NXWzNmoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFc6QDZpPkKyOVg4QTh4PD;hQi=>
human MDA435/LCC6MDR cells NYXCN5llTnWwY4Tpc44h[XO|YYm= MV61JIRigXN? MV\Nc4R2dGG2aX;uJI9nKFBvZ4CgLJVvc26xd36gc5Jq\2mwKTD0doFve2[nY4Tl[EBqdiCqdX3hckBOTEF2M{WvUGNEPk2GUjDj[YxteyCjc4Pld5Nm\CCjczDy[ZZmenOrbnegdIFkdGm2YYjlcEBz\XOrc4ThcoNmKG2nYYP1doVlKGG|IHPlcIwhe3W{dnn2ZYwh[W[2ZYKgOUBl[Xm|IHL5JG1VWyCjc4PhfUwhTUN3ME2wMlA{OiEQvF2u NHywc5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4OVE6PSd-MkW5PFUyQTV:L3G+
human PBMC MmX1VJJwdGmoZYLheIlwdiCjc4PhfS=> NW\ZOlBuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRm1ENCCLQ{WwQVEzKG6P M1vBNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3Nke5N|A6Lz5zNU[3PVMxQTxxYU6=
mouse T cells M322SGZ2dmO2aX;uJIF{e2G7 M3TCcFQh\GG7cx?= NF;ub|FKdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCvb4Xz[UBVKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geJdwKHejeTDtbZhm\CCueX3wbI9kgXSnIILlZYN1cW:wIHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[XluIHnjOVA:OTFibl2= M4XCd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi0PVg4Lz5{Mkm4OFk5PzxxYU6=
human splenocytes M{LDXWZ2dmO2aX;uJIF{e2G7 MkjyOEBl[Xm| NIjzbpFKdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckB{eGynbn;jfZRmeyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFt|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCjZoTldkA1KGSjeYOgZpkh[Wyub3flcolkKG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44h[XO|YYmsJGlEPTB;MUCgcm0> NVm4XFQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlc5OzFpPkG5PFI4QDNzPD;hQi=>
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human Jurkat cells M3W0OGZ2dmO2aX;uJIF{e2G7 NWHn[JJTOSCq M1zpeGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[W62aT3DSFMwS0R{ODDhcpRq[m:meT3pcoR2[2WmIFnMMVIheHKxZIXjeIlwdiC2cnXheIVlKG[xcjCxJIhzKGKnZn;y[UBidnSrLVPEN{9ETDJ6IHHueIljd2S7IHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB4IHjyd{BjgSCrbX31co9{fXCycnXzd4lwdiCjc4PhfUwhTUN3ME21JI5O NVvCd2dvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5N|M4QTVpPkG5PVM{Pzl3PD;hQi=>
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A2780/ADR M{GyeWZ2dmO2aX;uJIF{e2G7 NILuZ|FKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNW2nZHnheIVlKG23bITp[JJ2\yC{ZYPpd5RidmOnIHnuJIFlemmjbYnjbY4uemW|aYP0ZY51KGi3bXHuJGEzPzhyL1HEVkBk\WyuczDifUBk[WylZXnuJGFOKGG|c3H5MEBKSzVyIE2gNU41KM7:TT6= NHnrfFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1NFg{PCd-MUmyOVA5OzR:L3G+
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C2C12 NYjKb5Z3TnWwY4Tpc44h[XO|YYm= NVPpXZBGUW6qaXLpeIlwdiCxZjDz[ZJ2dSC5aYTo[JJif2GuLXnu[JVk\WRiY3XscEBlcW[oZYLlcpRq[XSrb36gbY4hdW:3c3WgR|JEOTJiY3XscJMh[XO|ZYPz[YQh[XNibYnvd4lvKGinYY\5JINp[WmwIHX4dJJme3Orb36u NEXLNFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC4NVg2PSd-MkCwPFE5PTV:L3G+
HuH7 NX;zdFlNSW62aY\pdoFtKGG|c3H5 MWG0PEBpenN? Mn7wRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNXKHO3YoT5dIUhOWJiQ3;uNUBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckB3cXKjbDDSUmEhdGW4ZXygZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygSWM2OCB;IECuNlEh|ryPLh?= NHW2XpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG3Olg6PCd-MkCxO|Y5QTR:L3G+
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T-cells MVLDfZRwfG:6aXPpeJkh[XO|YYm= MVm0PEBpenN? NV3tNHN1S3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJHQh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygR2M2OCB;IEG1MlgzKM7:TT6= NF21OmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzNVExPCd-MkGxN|EyODR:L3G+
HCT15 MofTSpVv[3Srb36gZZN{[Xl? M2q0flExKHWP NG\mWlRKdmirYnn0bY9vKG:oIF3EVlEhcW5iaIXtZY4hUEOWMUWgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4Ft[2Wrbj3BUUBm\m[udYigZZQhOTBidV2gZpkh\my3b4Lld4NmdmOnIHHzd4F6Ng>? MorOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEi0OlEoRjJzM{S4OFYyRC:jPh?=
T-cells NHjzbnVKdW23bn;zeZBxemW|c3n2[UBie3OjeR?= MkHrPVYhcHK| M{XHV2ludXWwb4P1dJBz\XO|aY\lJIFkfGm4aYT5JIlvKEKDTFKvZ{9EPTeETD:2JI1wfXOnIGSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjbHzvZY51cWenbj3pcoR2[2WmIHPlcIwheHKxbHnm[ZJifGmxbjDh[pRmeiB7NjDodpMh[nlibXXhd5VzcW6pIGuzTH11cHmvaXTpcoUhfXC2YXvlJIJ6KG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44h[XO|YYmsJGlEPTBiPTCwMlAyPCEQvF2u NGLoO3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1OlUzPCd-MkG0OVY2OjR:L3G+
mouse spleen cells NETIOIdHfW6ldHnvckBie3OjeR?= NFi5NlFKdmirYnn0bY9vKG:oIIPldolv\SCyYXztbZRwgWy2cnHud4ZmemG|ZTDpckBud3W|ZTDzdIxm\W5iY3XscJMv NVS5VXdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OVY2OjRpPkKxOFU3PTJ2PD;hQi=>
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CCRF-CEM/VCR1000 MnHlSpVv[3Srb36gZZN{[Xl? MnzhNlQxKHOnY4O= M3\McGlvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4udWWmaXH0[YQh\GG3bn;yeYJq[2mwIHXm[ox2gCCocn;tJIh2dWGwIFPDVmYuS0WPL2\DVlExODBiY3XscJMh[W[2ZYKgNlQxKHOnY4OgZpkhTkGFUzDmcI94KGO7dH;t[ZRzcWNiYX7hcJl{cXNuIFnDOVAhRSByLkGwNlM{KM7:TT6= NEXU[FU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS1NlQyOid-MkK0OVI1OTJ:L3G+
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mouse lymph node cells NYTUWplUS3m2b4TvfIlkcXS7IHHzd4F6 NXjjPIFSPDhiaILz MkjxR5l1d3SxeHnjbZR6KGGpYXnud5QhSmGuYj;jJI1wfXOnIHz5cZBpKG6xZHWgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDFyLkGg{txONg>? MlH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OUS2NVYoRjJ{NEm0OlE3RC:jPh?=
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Assay
Methods Test Index PMID
Western blot Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3 ; Cyp-D / AIF / β-actin ; IL-8 / MCP-1 ; Actin / Smac ; β-actin / FIPV-N 12644839 29328412 9602633 12427831 22546085
Growth inhibition assay NPC death 20181586
IHC Th22 / Th17 / Th1 / M1 / Th2 ; Tregs / Bregs / pDCs / M2 ; Ki 67 / Fas-FasL ; IFN-γ / TNF-α ; WT-PCLS / FXRKO-PCLS 30916387 10931158 26482353
Immunofluorescence Human gingival fibroblast cells ; Human gingival fibroblast cells ; Calcein ; Rhod-Dex / Calcein ; cadherin-9 / vimentin 27857765 23593196 20584761 17668045
In vivo Cyclosporin A is an immunosuppressive agent that is effective following either parenteral or oral administration in mice, rat and guinea pigs. [6], is clinically used in organ transplantation to prevent rejection. [7]

Protocol (from reference)

Kinase Assay:[3]
  • Phosphatase Assay:

    Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described.

Cell Research:[3]
  • Cell lines: Jurkat cells (clone J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1 hr
  • Method: Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.
Animal Research:[6]
  • Animal Models: Rat
  • Dosages: 45 mg/kg
  • Administration: orally

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 1202.61
Formula

C62H111N11O12

CAS No. 59865-13-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1C(=O)N(CC(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)NC(C(=O)NC(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N1)C(C(C)CC=CC)O)C)C(C)C)C)CC(C)C)C)CC(C)C)C)C)C)CC(C)C)C)C(C)C)CC(C)C)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05056779 Not yet recruiting Drug: Nemolizumab|Drug: CD14152 placebo Moderate-to-severe Atopic Dermatitis Galderma R&D January 2022 Phase 3
NCT04989686 Not yet recruiting Other: Microsampling Immunosuppression Children''s Hospital of Philadelphia December 2021 --
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

Answer:
Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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