Cyclosporin A (NSC 290193)

For research use only.

Catalog No.S2286 Synonyms: Cyclosporine A, Cyclosporine, Ciclosporin, CsA

50 publications

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.

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Selleck's Cyclosporin A (NSC 290193) has been cited by 50 publications

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Biological Activity

Description Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.
Targets
calcineurin [1]
(Cell-free assay)
7 nM
In vitro

Cyclosporin A binds to the cyclophilin (immunophilin) in T cells[1] , forms a Cyclophilin-Cyclosporin A complex which the binds to and inhibits calcineurin. [2] Cyclosporin A inhibits calcineurin with IC50 of 7 nM[3], then blocks the nuclear translocation of NF-AT. [4] Cyclosporin A also affects mitochondria by preventing the MTP (mitochondrial permeability transition pore) from opening with an IC50 of 39 nM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells MUTDfZRwfG:6aXRCpIF{e2G7 MoTOR5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFi0NmUh[2WubIOgZZN{\XO|ZXSgZZMhcW62cnHj[YxtfWyjcjDBWHAhdGW4ZXysJHREPTB;NEKg{txO NFLYb449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWwNlA2QCd-MUm1NFIxPTh:L3G+
mouse L929 cells M4DRcGN6fG:2b4jpZ:Kh[XO|YYm= MVizJIRigXN? MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMPVI6KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHMh[XO|YYmsJGlEPTB;M{Cg{txO Mm\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human Huh-Mono cells NYO1XXI2S3m2b4TvfIlkyqCjc4PhfS=> NXnQNlJ4OyCmYYnz NV7UPFZFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqLV3vco8h[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3Ojef-8kEBESzVyPUK0JO69VQ>? M3H1T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
MDCK cells M4Ps[2N6fG:2b4jpZ:Kh[XO|YYm= M3v4OGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQ1ugZ4VtdHNuIGTDOVA:QS57IN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
human LCC-6 cells NHO5cpJEgXSxdH;4bYPDqGG|c3H5 NFf6Tlg4OiCq NUjTdVl4S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVEOFLU[gZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUiuN{DPxE1? NVHmVIY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNlA1ODJpPkKyN|IxPDB{PD;hQi=>
human HeLa cells MUnDfZRwfG:6aXRCpIF{e2G7 MmfhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{whXEN3ME24MlMh|ryP M3uxNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells M1rZbmZ2dmO2aX;uJIF{e2G7 NX3Jb3liOjRiaB?= MlPQRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fCCQaXzlJJZqenW|IHnu[oVkfGWmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KF[ncn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygVm5CKHO7boTo[ZNqeyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRThizszN NUPnSIU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVEzQDZpPkG5OFUyOjh4PD;hQi=>
human Huh-9-13 cells M{j6eGN6fG:2b4jpZ:Kh[XO|YYm= M2O3TVMh\GG7cx?= MlzYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNTlvMUOgZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5Nige,:jDDDR|UxRTdizszN M37HTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH6 cells NFzzeoZEgXSxdH;4bYPDqGG|c3H5 M3:zb|Mh\GG7cx?= M3HsNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDZiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhS0N3ME23JO69VQ>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human Huh5-2 cells MV3DfZRwfG:6aXRCpIF{e2G7 M{nmN|Mh\GG7cx?= MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYg2NTJiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUAtKEOFNUC9OkDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human HuH7 cells Ml\RR5l1d3SxeHnjxsBie3OjeR?= NY\KO2RxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[Yxm[XOnIH;mJIxi[3SjdHWg[IVpgWS{b3flcoF{\SxiSVO1NF02NjhzIN88US=> NFfnPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm0N|M6OCd-MkC5OFM{QTB:L3G+
human 2008 cells NVXNNpFITnWwY4Tpc44h[XO|YYm= NEHJW4ZKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEGgbY4hcHWvYX6gNlAxQCClZXzsd{whUUN3ME20Mlc5KM7:TR?= NYP4b|VXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3NFc5QDRpPkG4O|A4QDh2PD;hQi=>
human HeLa cells NFfaSZdHfW6ldHnvckBie3OjeR?= MnHIRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTHBXNTF6IHnu[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB5IHThfZPwxIxiVFO1NF01NjZizszN M4X1VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
leukemia CEM cells M4fXV2Z2dmO2aX;uJIF{e2G7 NX;0dWVFUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEi3bXHuJG1FWjFiUD3HcJlkd3C{b4TlbY4hSUKFIGTyZY5{eG:{dHXyJJV{cW6pIHzleYtmdWmjIFPFUUBk\WyuczygTWM2OD1|LkSg{txO MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjN4MUO4O{c,OTJ|NkGzPFc9N2F-
human Caco2 cells NH7BTpFHfW6ldHnvckBie3OjeR?= NHfIcWRvcGmkaYTpc44hd2ZiUD3ndEBqdiCqdX3hckBE[WOxMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\Gmpb4jpckBm\m[udYisJGlEPTB;Mj65JO69VQ>? NW[4UXBVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPFI1PThpPkKzN|gzPDV6PD;hQi=>
human MDA435/LCC6MDR cells MmnqR5l1d3SxeHnjxsBie3OjeR?= NXPXT2gxPzKq NHXDeXREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEF2M{WvUGNEPk2GUjDj[YxteyCneIDy[ZN{cW6pIF3EVkBi\nSncjC3NkBpenQxvJygTWM2OD1{Lkig{txO NGLQTG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNFQxOid-MkKzNlA1ODJ:L3G+
guinea pig ventricular myocytes MnrrSpVv[3Srb36gZZN{[Xl? NFLL[5pKdmirYnn0bY9vKG:oIFyteJlx\SClYXzjbZVuKGOqYX7u[YwhdWWjc4Xy[YQhfXOrbnege4hwdGVvY3XscEBx[XSlaDDjcIFueCCrbjDneYlv\WFicHnnJJZmdnS{aXP1cIFzKG27b3P5eIV{NCCLQ{WwQVIh|ryP M3jDSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{[xNFAxLz5{Mke2NVAxODxxYU6=
KB/MDR cell M37sPWZ2dmO2aX;uJIF{e2G7 MXK3NkBp NWrOfZFXUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEL13EVkBk\WyuIHzpcoUh[W[2ZYKgO|IhcHJib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0yNjVizszN M3L1elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
A2780/ADR cells NYjmN4g{TnWwY4Tpc44h[XO|YYm= NEDlXlJKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvKGW6cILld5Nm\CCrbjDBNlc5OC:DRGKgZ4VtdHNiYomgZ4Ft[2WrbjDBUUBie3Ojef-8kEBKSzVyPUGuOFEzPTRizszN NU\RXYFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells NYXDcIZbTnWwY4Tpc44h[XO|YYm= M1fyRlEh|ryP Mk\vO|IhcA>? MkK3VI91\W62aXH0bY9vKG:oIHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGGpYXnud5Qh\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKEt3NkKvVlch[2WubIOgZZN{\XO|ZXSgZZMh\G:6b4L1ZolkcW5iSVO1NEBifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD63JO69VQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|NEC0NUc,OjV4M{SwOFE9N2F-
LLC-PK1 epithelial cells NX7teYdYTnWwY4Tpc44h[XO|YYm= M2PDWWlvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4tKG2xdYPlJGwudWS{MXKg[ZhxemW|c3XkJIlvKEyOQz3QT|Eh\XCrdHjlcIlidCClZXzsd{B2e2mwZzDjZYxk\WmwLVHNJJBwdGG{aYPheIlwdiCjc4Phfg+9lCCLQ{WwQVAvPyEQvF2= M3\6blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
mouse L5178 cells MoLXSpVv[3Srb36gZZN{[Xl? M2fKRlIxKG2rboO= NVrSOlhoUW6qaXLpeIlwdiCxZjDoeY1idiCDQlPCNU1u\WSrYYTl[EBzcG:mYX3pcoUhOTJ|IHXm[ox2gCCrbjDtc5V{\SCONUG3PEBk\WyuczDlfJBz\XO|aX7nJIh2dWGwIF3EVlEh[W[2ZYKgNlAhdWmwczDifUBHSUOVIHHuZYx6e2m|LDDJR|UxRTBwNkWg{txO MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG3OFUoRjJzM{SxO|Q2RC:jPh?=
KB cell MX3GeY5kfGmxbjDhd5NigQ>? MYXJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgT2Ih[2WubDDsbY5mKGGodHXyJFczKGhib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0xNjZizszN M162eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
mouse mammary carcinoma cell line EMT6/AR 1.0 MVPGeY5kfGmxbjDhd5NigQ>? M2KxUnJmfmW{c3HsJIVn\mWldDDvckB1cGViYXPjeY12dGG2aX;uJI9nKFt|SG2tJIRifW6xcoXibYNqdiCrbjDtc5V{\SCvYX3tZZJ6KGOjcnPpco9u[SClZXzsJIxqdmViRV3UOk9CWiBzLkCsJGlEPTB;MD60OEDPxE1? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB7OE[3NUc,OTByOUi2O|E9N2F-
CK1 cells M17h[WZ2dmO2aX;uJIF{e2G7 M{ThS2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1|MzDpcoZm[3SnZDDpckBEUzFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXNuIGTDOVA:OC5|IN88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
HEK293 cells NGD4O4dHfW6ldHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKE:DVGCxRlEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KHC{b4TlbY4udWWmaXH0[YQheGm2YY\hd5RifGmwIIXweIFs\e,:jDDLbV0xNjJizszN NF7K[4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4O|k5Pid-MkK1PFc6QDZ:L3G+
human MDA435/LCC6MDR cells NIHyfYFHfW6ldHnvckBie3OjeR?= M2jRZ|Uh\GG7cx?= NVf2XGlRVW:mdXzheIlwdiCxZjDQMYdxKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gbJVu[W5iTVTBOFM2N0yFQ{\NSHIh[2WubIOgZZN{\XO|ZXSgZZMhemW4ZYLzbY5oKHCjY3zpeIF5\WxicnXzbZN1[W6lZTDt[YF{fXKnZDDhd{Bk\WyuIIP1dpZqfmGuIHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XluIFXDOVA:OC5yM{Kg{txONg>? MlLMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human PBMC NFnENHJRem:uaX\ldoF1cW:wIHHzd4F6 NV;i[XdCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRm1ENCCLQ{WwQVEzKG6P NGLabGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[3PVMxQSd-MUW2O|k{ODl:L3G+
mouse T cells MknqSpVv[3Srb36gZZN{[Xl? NXPFTIVFPCCmYYnz MVjJcY12dm:|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBud3W|ZTDUJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidIfvJJdigSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKGGodHXyJFQh\GG7czDifUBOXFRiYYPzZZktKGmlNUC9NVEhdk1? M3HQPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi0PVg4Lz5{Mkm4OFk5PzxxYU6=
human splenocytes MVfGeY5kfGmxbjDhd5NigQ>? M4nVfFQh\GG7cx?= MYPJcY12dm:|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBpfW2jbjDzdIxmdm:leYTld{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHs{UF22aIntbYRqdmViaX7jc5Jxd3KjdHnvckBi\nSncjC0JIRigXNiYomgZYxtd2enbnnjJI1qgGWmIHz5cZBpd2O7dHWgdoVi[3Srb36gZZN{[XluIFnDOVA:OTBibl2= NH;GNWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUiyO|g{OSd-MUm4Nlc5OzF:L3G+
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MDA-435/LCC6 (Pgp-negative) M13tc2Z2dmO2aX;uJIF{e2G7 NWD2RnVQTW[oZXP0JI9vKHKndnXyd4FtKG:oIFTPXEBi[2O3bYXsZZRqd25iaX6gUWRCNTR|NT;MR2M3KCiSZ4CtcoVo[XSrdnWpJINmdGy|LjygSWM2OCB;IECuO{DPxE1w NXTWXlVTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO|g1OTR|PD;hQi=>
MDA-435/LCC6 NXTpO2R3T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= M1XvWWdzd3e2aDDpcohq[mm2b4L5JIFkfGm4aYT5JI9vKE2GQT20N|UwVEOFNjCoVIdxNXCxc3n0bZZmMSCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIx{NixiSVO1NEA:KDFizszNMi=> NIPKV|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
MDA-435/LCC6-MDRI NHf5eZFIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MoHNS5Jwf3SqIHnubIljcXSxcomgZYN1cX[rdImgc44hVUSDLUSzOU9NS0N4LV3EVmkhMFCpcD3u[YdifGm4ZTmgbJVu[W5iYoLlZZN1KGOjbnPldkBk\Wyucz6sJGlEPTBiPTCxMlEh|ryPLh?= M4P1[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
BTI-TN5B1-4 NG\SSYtHfW6ldHnvckBie3OjeR?= MlrQRXRRKGi7ZILvcJl{cXNiaX6gcYVu[nKjbnWg[pJi[3Srb36g[pJwdSCKaXfoJGZqfmViKFLUTU1VVjWEMT20LUBk\WyuczygT40hRSByLkG3JO69VS5? NHTGUHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OVY5PCd-MUG3PFU3QDR:L3G+
Jurkat M3uxbWZ2dmO2aX;uJIF{e2G7 NVezUGNZUW6qaXLpeIlwdiCxZjDwdo9lfWO2aX;uJI9nKEmOLUKgd4VkemW2aX;uJIlvKEq3cnvheEBk\WyuczygTWM2OCB;IECuNFA4KM7:TT6= NYjsXFF1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG4PFE6QDRpPkGxPFgyQTh2PD;hQi=>
Jurkat MWrGeY5kfGmxbjDhd5NigQ>? MV3Jcohq[mm2aX;uJI9nKHC{b3T1Z5Rqd25ib3[gZoV1[S2pYXzhZ5Rwe2mmYYPlJGlNNTJiUlfBJIlvKEq3cnvheEBk\WyuczygTWM2OCB;IECuNFA5KM7:TT6= M1nsOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOEixPVg1Lz5zMUi4NVk5PDxxYU6=
LLC-PK1 M12zO2Z2dmO2aX;uJIF{e2G7 NXq5PJkzcW6lcnXhd4UhcW5iVnnuZoxie3SrbnWgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gbY4hVUSUMT3lfJBz\XO|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOS5|IN88UU4> M3;rbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[xNVE{Lz5zMUm2NVEyOzxxYU6=
LLC-PK1 M1f5bmZ2dmO2aX;uJIF{e2G7 NVrGSnJrcW6lcnXhd4UhcW5iQ3HsZ4Vqdi2DTTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUA1NjZ4IN88UU4> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTl4MUGxN{c,OTF7NkGxNVM9N2F-
MDCK NWqyVZM2TnWwY4Tpc44h[XO|YYm= MkDTbY5pcWKrdHnvckBw\iCGaXfvfIlvKHS{YX7z[ZBqfGinbHnhcEB1emGwc4DvdpQhMGKjc3HsJJRwKGGyaXPhcEkhcW5iTVTSNU1mgHC{ZYPzbY5oKE2GQ1ugZ4VtdHNuIFvpJF0hOi5zODFOwG0v MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF|NEm0OUc,OTJzM{S5OFU9N2F-
MDCK M4PvdmZ2dmO2aX;uJIF{e2G7 NXG3VZNNcW6qaXLpeIlwdiCxZjDWbY5jdGG|dHnu[UB1emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JEhj[XOjbDD0c{BieGmlYXypJIlvKE2UUEKt[ZhxemW|c3nu[{BOTEONIHPlcIx{NCCNaTC9JFgvOTFizszNMi=> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF|NEm0Okc,OTJzM{S5OFY9N2F-
LLC-PK1 Mn\TSpVv[3Srb36gZZN{[Xl? NU\q[VRRcW6lcnXhd4UhcW5iQ3HsZ4Vqdi2DTTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBu\HJzYj3lfJBz\XO|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOy53IN88UU4> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjJ|NUK2O{c,OTJ{M{WyOlc9N2F-
LLC-PK1 NH;4VYpHfW6ldHnvckBie3OjeR?= NVjt[mFxcW6lcnXhd4UhcW5iQ3HsZ4Vqdi2DTTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBu\HJzYT3lfJBz\XO|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOy54NTFOwG0v MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjJ|NUK2O{c,OTJ{M{WyOlc9N2F-
HL-60 MlfzSpVv[3Srb36gZZN{[Xl? MoDCTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIH\vdo16dHCncITp[IUhemWlZYD0c5IhME[SUjmgc4YhcHWvYX6gcIV2c2WvaXGgTGwuPjBiY3XscJMtKEmFMkCgQUA{NjZizszNMi=> NGLPb4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkO2NVM5QCd-MUKzOlE{QDh:L3G+
High Five cells MUTGeY5kfGmxbjDhd5NigQ>? MmXlbY5pcWKrdHnvckBw\iCWYYXyc4Np\W6xZHXvfJlkcG:uYYTlJJVxfGGtZTjDd2EhOzC3TTygN|AhLSCxZjDjc451em:uKTDpckBu\W2kcnHu[UB3\XOrY3zld{BxemWyYYLl[EBnem:vIFjp[4ghTmm4ZTDj[YxteyCrbn\lZ5Rm\CC5aYToJJRp\SCDQlPCNVEh[mGldXzveolzfXNuIFvpJF0hQS53IN88UU4> MoD5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MESyN|koRjF{NEC0NlM6RC:jPh?=
MDCKII MoSzSpVv[3Srb36gZZN{[Xl? MX7pcohq[mm2aX;uJI9nKEOncnn2ZZN1[XSrbjD1dJRic2ViaX6gU2FVWDJvZYjwdoV{e2mwZzDNSGNMUUliY3XscJMtKEurIE2gNE4zOzhizszNMi=> NGHYZ5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkWzPFgyOyd-MUK1N|g5OTN:L3G+
Caco-2 NHu1S|BHfW6ldHnvckBie3OjeR?= M1ruNpRz[W6|ZYDpeIhmdGmjbDD0doFve3CxcoSgc4Yh\Gmpb4jpckAp[mG|YXygeI8h[XCrY3HsLUBqdiCFYXPvMVIh[2WubIOsJGtqKD1iMD6zOUDPxE1w NFTtcWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[zOlE2Oyd-MUK2N|YyPTN:L3G+
SK-E2 MUPGeY5kfGmxbjDhd5NigQ>? MWLpcoNz\WG|ZTDpckBjd2SrcImgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gLGJw\GmyeUqgNE4zKHWPKTDpckBUUy2HMjDj[YxteyBqZYjwdoV{e2mwZzDCV2VRMSxiSVO1NEA:KDdwNTFOwG0v NELINm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkezPVc2QSd-MUK3N|k4PTl:L3G+
SK-E2 M3HJR2Z2dmO2aX;uJIF{e2G7 MXTpcoNz\WG|ZTDpckBlcWi7ZILv[ox2d3Knc3PlbY4hcW62cnHj[YxtfWyjcjDhZ4N2dXWuYYTpc44hMGSraInkdo9ndHWxcnXzZ4VqdjpiMTD1UUkhcW5iU1utSVIh[2WubIOgLIV5eHKnc4PpcochSlOHUDmsJGlEPTBiPTC3Mlgh|ryPLh?= NHTJS489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkezPVc2QSd-MUK3N|k4PTl:L3G+
HEK293 M3ezcWZ2dmO2aX;uJIF{e2G7 Mmn2bY5pcWKrdHnvckBw\iCSaHHscI9q\GmwIIXweIFs\SBqUHjhcIxwcWSrbkqgNUB2VSliaX6gU2FVWC2FLXX4dJJme3OrbnegTGVMOjl|IHPlcIx{NCCNaTC9JFAvOiEQvF2u MonSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3M{C5NFcoRjF2NUOwPVA4RC:jPh?=
T-cells NEmzW4lHfW6ldHnvckBie3OjeR?= NH\3O5pKdmirYnn0bY9vKG:oIHHscI9o\W6rYzDj[Yxtey2|dHnteYxifGWmIIDyc4xq\mW{YYTpc44hcW5ibX;1d4UhXCClZXzsd{BjgSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKG2ndHjv[EwhUUN3MDC9JFAvODN{IN88UU4> MlHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3OUOxPFIoRjF2NUmzNVgzRC:jPh?=
T-cells MYfGeY5kfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGGubH;n[Y5q[yClZXzsd{1{fGmvdXzheIVlKHC{b3zp[oVz[XSrb36gbY4hcHWvYX6gWEBk\WyuczDifUBucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJI1mfGixZDygTWM2OCB;IECuNFc1KM7:TT6= M1HUcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUmzNVgzLz5zNEW5N|E5OjxxYU6=
PMBC MYfGeY5kfGmxbjDhd5NigQ>? M{TQV|czKGi{cx?= MoraSYZn\WO2IH;uJJBzd2yrZnXyZZRqd25ib3[gbJVu[W5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyBqUF3CR{kh[XRiN{KgbJIhfXOrbnegUXRVKGG|c3H5MEBKSzVyIE2gPE46PyEQvF2u NXX0NINQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWyNFMyPjNpPkG1NlA{OTZ|PD;hQi=>
mouse spleen cells NXe5VFFUS3m2b4TvfIlkcXS7IHHzd4F6 NG\vUJdEgXSxdH;4bYNqfHliYXfhbY5{fCCEQVzCM4MhdW:3c3Wgd5Bt\WWwIHPlcIx{NCCFQ{WwJF0hOS5|NTFOwG0v MmjWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyOEG3OlEoRjF5MEixO|YyRC:jPh?=
MDCK2 NVrocFBbTnWwY4Tpc44h[XO|YYm= MmDnTY5pcWKrdHnvckBw\iCqdX3hckBOTFJzLXTldIVv\GWwdDDhZ4N2dXWuYYTpc44hd2ZiY3HsZ4Vqdi2DTTDlfJBz\XO|ZXSgbY4hVUSFS{KgZ4VtdHNuIFnDOVAhRSB7LkO0JO69VS5? Mmm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkSzNlcoRjF5Nk[0N|I4RC:jPh?=
HEK293 MVLGeY5kfGmxbjDhd5NigQ>? MlPFTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTUGgUFY2Wi:WTU[gUFM{QUNxVF23JGY4OjhibYX0ZY51KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIEWwKUBqdmirYnn0bY9vKG:oIHPyc5N{KGyrbnvpcoch[nliaX3teY5w[myxdDygRYN1cX[rdImgQUAxNjVizszNMi=> NXzJflJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ|PD;hQi=>
HEK293 MVLGeY5kfGmxbjDhd5NigQ>? M1vJRWlvcGmkaYTpc44hd2ZiY4Lvd5MudGmwa3nu[{Bw\iCqdX3hckBR\3BiVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDBwNjFOwG0v Mnq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6NEi1OlMoRjF5OES4OVY{RC:jPh?=
HEK293 MYHGeY5kfGmxbjDhd5NigQ>? NXvMV4JXUW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVNVOTl7Uj;UUVYhVDN|OVOvWG04KEZ5MkjDJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{A2OCViaX7obYJqfGmxbjDv[kBkem:|czDsbY5scW6pIHL5JIludXWwb3Lsc5QtKEGldHn2bZR6KD1iMT6xJO69VS5? NYPmbXhoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ|PD;hQi=>
HEK293 MlT0SpVv[3Srb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPNjDGN|Q{Wi:WTU[gUFM{QUNxVF23JGY4OjiFIH31eIFvfCCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHTyeYchdGW4ZXygZ4F2e2mwZzC1NEUhcW6qaXLpeIlwdiCxZjDjdo9{eyCuaX7rbY5oKGK7IHntcZVvd2Kub4SsJGFkfGm4aYT5JF0hOTFizszNMi=> M{Gz[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OES4OVY{Lz5zN{i0PFU3OzxxYU6=
HEK293 MWTGeY5kfGmxbjDhd5NigQ>? MoSzTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTUWgTVMxPlJxVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDF3IN88UU4> NXjsVVl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ|PD;hQi=>
MDCK2 NWjHUVFHTnWwY4Tpc44h[XO|YYm= MWSxNEB2VQ>? NGDFPIk1OCCvaX7z NVH3WVNQUW6qaXLpeIlwdiCxZjDoeY1idiC5aXzkJJR6eGViUHfwJIV5eHKnc4Pl[EBqdiCPRFPLNkBk\WyuczDhd5Nme3OnZDDhd{Bk[WylZXnuMWFOKGGlY4XteYxifGmxbjDheEAyOCC3TTDh[pRmeiB2MDDtbY5{Ng>? MlrRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NkSxO|AoRjF5OU[0NVcxRC:jPh?=
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BT-37 MXfxTHRUKGG|c3H5 NEPZ[nlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN|ch[2WubIOu M4f2XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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MG 63 (6-TG R) MnrrdWhVWyCjc4PhfS=> M17sTJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucz6= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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OHS-50 MXLxTHRUKGG|c3H5 MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy|Lh?= NEK3Vmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Rh30 NGToZoxyUFSVIHHzd4F6 MVPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFMxKGOnbHzzMi=> NWXRPJk3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 M2TrbZFJXFNiYYPzZZk> MnjsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog1OSClZXzsd{4> Mn7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MWLxTHRUKGG|c3H5 M3jsRZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzzMi=> M4TDeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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TC32 M1rRbJFJXFNiYYPzZZk> MoK2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsd{4> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
C57BL/6 mouse lymph node cells M1XlNmludXWwb4P1dJBz\XO|aY\lJIF{e2G7 NXTUV3QyOjRiaILz MmL5TY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hSzV5QlyvOkBud3W|ZTDsfY1xcCCwb3TlJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkWg{txONg>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh5MUi0OEc,Ojl6N{G4OFQ9N2F-
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; 

PubMed: 12644839     


Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm).

Cyp-D / AIF / β-actin; 

PubMed: 29328412     


Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. *P<0.05, **P<0.01, ***P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor.

IL-8 / MCP-1; 

PubMed: 9602633     


Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity.

Actin / Smac; 

PubMed: 12427831     


Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release.

β-actin / FIPV-N; 

PubMed: 22546085     


Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.

12644839 29328412 9602633 12427831 22546085
Growth inhibition assay
NPC death; 

PubMed: 20181586     


Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).

20181586
IHC
Th22 / Th17 / Th1 / M1 / Th2; 

PubMed: 30916387     


(a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Tregs / Bregs / pDCs / M2; 

PubMed: 30916387     


(b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Ki 67 / Fas-FasL; 

PubMed: 30916387     


(c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

IFN-γ / TNF-α; 

PubMed: 10931158     


Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat.

WT-PCLS / FXRKO-PCLS; 

PubMed: 26482353     


Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).

30916387 10931158 26482353
Immunofluorescence
Human gingival fibroblast cells; 

PubMed: 27857765     


Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole.

Human gingival fibroblast cells; 

PubMed: 27857765     


Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts.

Calcein; 

PubMed: 23593196     


E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm).

Rhod-Dex / Calcein; 

PubMed: 20584761     


Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM).

cadherin-9 / vimentin; 

PubMed: 17668045     


To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.

27857765 23593196 20584761 17668045
In vivo Cyclosporin A is an immunosuppressive agent that is effective following either parenteral or oral administration in mice, rat and guinea pigs. [6], is clinically used in organ transplantation to prevent rejection. [7]

Protocol

Kinase Assay:[3]
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Phosphatase Assay:

Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described.
Cell Research:[3]
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  • Cell lines: Jurkat cells (clone J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1 hr
  • Method: Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.
    (Only for Reference)
Animal Research:[6]
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  • Animal Models: Rat
  • Dosages: 45 mg/kg
  • Administration: orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (83.15 mM)
Water Insoluble
Ethanol ''''100 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1202.61
Formula

C62H111N11O12

CAS No. 59865-13-3
Storage powder
in solvent
Synonyms Cyclosporine A, Cyclosporine, Ciclosporin, CsA
Smiles CCC1C(=O)N(CC(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)NC(C(=O)NC(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N1)C(C(C)CC=CC)O)C)C(C)C)C)CC(C)C)C)CC(C)C)C)C)C)CC(C)C)C)C(C)C)CC(C)C)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3
NCT01762657 Withdrawn Drug: Oral Cyclosporine and Oral Lansoprazole|Drug: Placebos Type 1 Diabetes Perle Bioscience Inc. September 2014 Phase 3
NCT02130414 Completed Drug: Sandimmun® IV|Drug: CyCol® capsules Healthy Sigmoid Pharma July 2014 Phase 1
NCT01693393 Completed Drug: Cyclosporine A Sjögren´s Syndrome Charite University Berlin Germany March 2010 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • Answer:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID