Cyclosporin A (NSC 290193)

For research use only.

Catalog No.S2286 Synonyms: Cyclosporine A, Cyclosporine, Ciclosporin, CsA

54 publications

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.

Selleck's Cyclosporin A (NSC 290193) has been cited by 54 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.
Targets
calcineurin [1]
(Cell-free assay)
7 nM
In vitro

Cyclosporin A binds to the cyclophilin (immunophilin) in T cells[1] , forms a Cyclophilin-Cyclosporin A complex which the binds to and inhibits calcineurin. [2] Cyclosporin A inhibits calcineurin with IC50 of 7 nM[3], then blocks the nuclear translocation of NF-AT. [4] Cyclosporin A also affects mitochondria by preventing the MTP (mitochondrial permeability transition pore) from opening with an IC50 of 39 nM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells MlLlR5l1d3SxeHnjxsBie3OjeR?= NHz3PFNEgXSxdH;4bYNqfHliYXfhbY5{fCC{YYSgTFQzTSClZXzsd{Bie3Onc4Pl[EBieyCrboTyZYNmdGy3bHHyJGFVWCCuZY\lcEwhXEN3ME20NkDPxE1? NYLCc29rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NFIxPThpPkG5OVAzODV6PD;hQi=>
mouse L929 cells MWXDfZRwfG:6aXRCpIF{e2G7 M1\jdFMh\GG7cx?= MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMPVI6KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHMh[XO|YYmsJGlEPTB;M{Cg{txO Mn;2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human Huh-Mono cells NGXMTo1EgXSxdH;4bYPDqGG|c3H5 MmXrN{Bl[Xm| MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYguVW:wbzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F697zOIFPDOVA:OjRizszN MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
MDCK cells MmDYR5l1d3SxeHnjxsBie3OjeR?= M2j5O2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQ1ugZ4VtdHNuIGTDOVA:QS57IN88US=> MnjMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells M2jhdWN6fG:2b4jpZ:Kh[XO|YYm= MXS3NkBp Mk\yR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUGNENTZiY3XscJMh[W[2ZYKgO|IhcHK|LDDJR|UxRThwMzFOwG0> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ|MkC0NFI9N2F-
human HeLa cells M3G3R2N6fG:2b4jpZ:Kh[XO|YYm= NELGNXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{NCCWQ{WwQVgvOyEQvF2= M1XKUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells NYrPfY8zTnWwY4Tpc44h[XO|YYm= NUTlXpV7OjRiaB?= MnLPRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fCCQaXzlJJZqenW|IHnu[oVkfGWmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KF[ncn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygVm5CKHO7boTo[ZNqeyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRThizszN NHXp[3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVI5Pid-MUm0OVEzQDZ:L3G+
human Huh-9-13 cells M2TMTWN6fG:2b4jpZ:Kh[XO|YYm= NWXydGFTOyCmYYnz M{jvc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2cC17LUGzJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XoxvJygR2M2OD15IN88US=> M4GyZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH6 cells NVXMS49QS3m2b4TvfIlkyqCjc4PhfS=> MlfEN{Bl[Xm| MlXiR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPiClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBESzVyPUeg{txO MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human Huh5-2 cells NInI[VJEgXSxdH;4bYPDqGG|c3H5 NH:3PY0{KGSjeYO= MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYg2NTJiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUAtKEOFNUC9OkDPxE1? NX\HOVFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
human HuH7 cells Mn\HR5l1d3SxeHnjxsBie3OjeR?= NFvjWnlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IILlcIVie2Vib3[gcIFkfGG2ZTDk[Yh6\HKxZ3XuZZNmNCCLQ{WwQVUvQDFizszN NEj3dow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm0N|M6OCd-MkC5OFM{QTB:L3G+
human 2008 cells MU\GeY5kfGmxbjDhd5NigQ>? MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD12Lke4JO69VQ>? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDdyN{i4OEc,OTh5MEe4PFQ9N2F-
human HeLa cells MlXGSpVv[3Srb36gZZN{[Xl? M2\CVmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1zODDpcoZm[3SnZDDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNzDkZZl{97zOIGTDOVA:PC54IN88US=> Mmr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
leukemia CEM cells MYjGeY5kfGmxbjDhd5NigQ>? M1HCd2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDNSHIyKFBvR3z5Z49xem:2ZXnuJGFDSyCWcnHud5BwenSncjD1d4lv\yCuZYXr[Y1q[SCFRV2gZ4VtdHNuIFnDOVA:Oy52IN88US=> MmfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ|NkGzPFcoRjF{M{[xN|g4RC:jPh?=
human Caco2 cells Mlv3SpVv[3Srb36gZZN{[Xl? M3y1PI5pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJGNi[29{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBlcWexeHnuJIVn\my3eDygTWM2OD1{Lkmg{txO NU\NVmRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPFI1PThpPkKzN|gzPDV6PD;hQi=>
human MDA435/LCC6MDR cells NX;ndYRxS3m2b4TvfIlkyqCjc4PhfS=> NGHBc3E4Omh? MoH6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=> M1fHS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
guinea pig ventricular myocytes M3W2OWZ2dmO2aX;uJIF{e2G7 MlTUTY5pcWKrdHnvckBw\iCOLYT5dIUh[2GuY3n1cUBkcGGwbnXsJI1m[XO3cnXkJJV{cW6pIIfoc4xmNWOnbHygdIF1[2hiY3zhcZAhcW5iZ4XpcoViKHCrZzD2[Y51emmldXzhdkBugW:leYTld{whUUN3ME2yJO69VQ>? NWO0fG1MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OlExODBpPkKyO|YyODByPD;hQi=>
KB/MDR cell MnjsSpVv[3Srb36gZZN{[Xl? MnLnO|IhcA>? NFixO3dKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhU0JxTVTSJINmdGxibHnu[UBi\nSncjC3NkBpeiCxZjDkdpVoKGW6cH;zeZJmNCCLQ{WwQVEvPSEQvF2= NYHOVJlLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFE6QTFpPkG1OFgyQTlzPD;hQi=>
A2780/ADR cells MUfGeY5kfGmxbjDhd5NigQ>? M4HpO2lvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4h\XiycnXzd4VlKGmwIFGyO|gxN0GGUjDj[YxteyCkeTDjZYxk\WmwIFHNJIF{e2G778{MJGlEPTB;MT60NVI2PCEQvF2= M4fiN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEmwNFk1Lz5zN{i5NFA6PDxxYU6=
human K562/R7 cells NX;aVXpmTnWwY4Tpc44h[XO|YYm= MWWxJO69VQ>? MnH4O|IhcA>? NUPVTGtFWG:2ZX70bYF1cW:wIH;mJIRwgG:{dXLpZ4lvNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIwWjdiY3XscJMh[XO|ZYPz[YQh[XNiZH;4c5J2[mmlaX6gTWM2OCCjdDCxJJVOKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE44KM7:TR?= NWXYdG9ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|QxPDFpPkK1OlM1ODRzPD;hQi=>
LLC-PK1 epithelial cells NHrBcndHfW6ldHnvckBie3OjeR?= NWj5OJhHUW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdixibX;1d4UhVC2vZIKxZkBmgHC{ZYPz[YQhcW5iTFzDMXBMOSCncHn0bIVtcWGuIHPlcIx{KHW|aX7nJINidGOnaX6tRW0heG:uYYLpd4F1cW:wIHHzd4F697zOIFnDOVA:OC55IN88US=> NGPQSoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[5PVM5QSd-MUK2PVk{QDl:L3G+
mouse L5178 cells NHPmdWpHfW6ldHnvckBie3OjeR?= NXPUNmY6OjBibXnudy=> MkGzTY5pcWKrdHnvckBw\iCqdX3hckBCSkOEMT3t[YRq[XSnZDDybI9l[W2rbnWgNVI{KGWoZnz1fEBqdiCvb4Xz[UBNPTF5ODDj[YxteyCneIDy[ZN{cW6pIHj1cYFvKE2GUkGgZYZ1\XJiMkCgcYlveyCkeTDGRWNUKGGwYXz5d4l{NCCLQ{WwQVAvPjVizszN NXXXUnhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFE4PDVpPkKxN|QyPzR3PD;hQi=>
KB cell NVXUU4ZVTnWwY4Tpc44h[XO|YYm= MnXYTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDKGOnbHygcIlv\SCjZoTldkA4OiCqIH;mJIRzfWdiZYjwc5N2emVuIFnDOVA:OC54IN88US=> MlXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2OEG5PVEoRjF3NEixPVkyRC:jPh?=
mouse mammary carcinoma cell line EMT6/AR 1.0 M{DYS2Z2dmO2aX;uJIF{e2G7 MUjS[ZZmenOjbDDl[oZm[3Rib36geIhmKGGlY4XteYxifGmxbjDv[kBcO0ifLTDkZZVvd3K3YnnjbY4hcW5ibX;1d4UhdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mKEWPVE[vRXIhOS5yLDDJR|UxRTBwNESg{txO M4jQclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEm4OlcyLz5zMEC5PFY4OTxxYU6=
CK1 cells M2[3eWZ2dmO2aX;uJIF{e2G7 M{nISmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1|MzDpcoZm[3SnZDDpckBEUzFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXNuIGTDOVA:OC5|IN88US=> NFrkNIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
HEK293 cells NGX5bJVHfW6ldHnvckBie3OjeR?= MknvTY5pcWKrdHnvckBw\iCRQWTQNWIyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCycn;0[YlvNW2nZHnheIVlKHCrdHH2ZZN1[XSrbjD1dJRic2YxvJygT4k:OC5{IN88US=> M{noelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUi3PVg3Lz5{MkW4O|k5PjxxYU6=
human MDA435/LCC6MDR cells NH:4NVFHfW6ldHnvckBie3OjeR?= NWjOW5ZjPSCmYYnz NH\zc2tOd2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpcocheGGlbHn0ZZhmdCC{ZYPpd5RidmOnIH3lZZN2emWmIHHzJINmdGxic4Xyeol3[WxiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5NigSxiRVO1NF0xNjB|MjFOwG0v M2PvXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC NHyzeYtRem:uaX\ldoF1cW:wIHHzd4F6 MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCETVOsJGlEPTB;MUKgcm0> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ5OUOwPUc,OTV4N{mzNFk9N2F-
mouse T cells MnHYSpVv[3Srb36gZZN{[Xl? MkPCOEBl[Xm| MUnJcY12dm:|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBud3W|ZTDUJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidIfvJJdigSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKGGodHXyJFQh\GG7czDifUBOXFRiYYPzZZktKGmlNUC9NVEhdk1? NGrucGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4OFk5Pyd-MkK5PFQ6QDd:L3G+
human splenocytes M{HsOmZ2dmO2aX;uJIF{e2G7 NFPROXA1KGSjeYO= M4DNWmludXWwb4P1dJBz\XO|aY\lJIFkfGm4aYT5JIlvKGi3bXHuJJNxdGWwb3P5eIV{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhYzOKXYTofY1q\GmwZTDpcoNwenCxcnH0bY9vKGGodHXyJFQh\GG7czDifUBidGyxZ3XubYMhdWm6ZXSgcJlueGixY4n0[UBz\WGldHnvckBie3OjeTygTWM2OD1zMDDuUS=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh{N{izNUc,OTl6Mke4N|E9N2F-
human T-cell NH72epJHfW6ldHnvckBie3OjeR?= NEjHSo1KdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gWE1k\WyuIIDyc4R2[3Srb36gc4YhdHmvcHjvb4lv\SCLTD2yMEBKSzVyPUiuNUBvVQ>? M2HROFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd3M{ezN|EoRjd3M{ezN|E9N2F-
human Jurkat cells MUTGeY5kfGmxbjDhd5NigQ>? MUWxJIg> NIrsOplCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKGi3bXHuJGp2emujdDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGGwdHmtR2Q{N0OGMkigZY51cWKxZImtbY5lfWOnZDDJUE0zKHC{b3T1Z5Rqd25idILlZZRm\CCob4KgNUBpeiCkZX\vdoUh[W62aT3DSFMwS0R{ODDhcpRq[m:meTDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMh[nliaX3teY5we3WycILld5Nqd25iYYPzZZktKEWFNUC9OUBvVQ>? NVnIXlE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5N|M4QTVpPkG5PVM{Pzl3PD;hQi=>
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MDA-435/LCC6 (Pgp-negative) NUDTRnJwTnWwY4Tpc44h[XO|YYm= Mnm4SYZn\WO2IH;uJJJmfmW{c3HsJI9nKESRWDDhZ4N2dXWuYYTpc44hcW5iTVTBMVQ{PS:OQ1O2JEhR\3BvbnXnZZRqfmVrIHPlcIx{NixiRVO1NEA:KDBwNzFOwG0v NYjlSHZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO|g1OTR|PD;hQi=>
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HL-60 M1jSfmZ2dmO2aX;uJIF{e2G7 NFHXUHRKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUHWvYX6g[o9zdXmucHXweIll\SC{ZXPldJRweiBqRmDSLUBw\iCqdX3hckBt\XWtZX3pZUBJVC14MDDj[YxteyxiSVOyNEA:KDNwNjFOwG0v M{XrWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN|g5Lz5zMkO2NVM5QDxxYU6=
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H69AR NIm5R2JHfW6ldHnvckBie3OjeR?= Ml\oN|AhdWmwcx?= MlvLTY5pcWKrdHnvckBw\iCDQlPDNUBqdiCqdX3hckBJPjmDUjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2GuY3XpckBCVSCuZY\lcJMheHKnaX7jeYJifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KGOjbHPlbY4hSU1iYXTkbZRqd25ibXXhd5Vz\WRiaX3t[YRq[XSnbImgZZQhPjBic3XjJJRqdWViaX70[ZJ3[WxiZn;yJFYxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOi57NzFOwG0v MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR5MU[1Okc,Ojh2N{G2OVY9N2F-
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H69AR MV3GeY5kfGmxbjDhd5NigQ>? MW[zNEBucW6| NWPLVFBxUW6qaXLpeIlwdiCxZjDBRmNEOSCrbjDoeY1idiCKNknBVkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3HsZ4Vqdi2DTTDl[oZtfXhicILlbY5kfWKjdHXkJIZweiB|MDDtbY5{KG[xbHzve4VlKGK7IHPhcINmcW5vQV2gZYRlcXSrb36gcYVie3W{ZXSgZZQhPjBic3Xjd{BqdnSnco\hcEBnd3JiNkCgcYlveyCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCzMlU{KM7:TT6= Mn3vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6NEG1NVMoRjJ6OESxOVE{RC:jPh?=
A673 MV;xTHRUKGG|c3H5 M{jYO5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy|Lh?= NVHpWZNIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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BT-37 NF\qT3RyUFSVIHHzd4F6 MWrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHNw MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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MG 63 (6-TG R) MX\xTHRUKGG|c3H5 NYHnfVE3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzzMi=> MorhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 M2TUNZFJXFNiYYPzZZk> NXn1SYZneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsd{4> M2W2[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M4\HW5FJXFNiYYPzZZk> M4DIXZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIOu MlvmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 MYHxTHRUKGG|c3H5 M2fQR5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaESxJINmdGy|Lh?= NESxXZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh30 MlHFdWhVWyCjc4PhfS=> NVXSXXZWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh|MDDj[Yxtey5? NIDjb4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 MoH5dWhVWyCjc4PhfS=> NHTxdodyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDRzIHPlcIx{Ng>? NV2z[21ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC M{G5U5FJXFNiYYPzZZk> NF;zZ2pyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsd{4> Mn3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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SK-N-MC M3zZPJFJXFNiYYPzZZk> Mo\FdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtey5? NFL3NJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 M3jnUpFJXFNiYYPzZZk> NYfGR4J[eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtey5? MknlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
C57BL/6 mouse lymph node cells MVPJcY12dm:|dYDwdoV{e2m4ZTDhd5NigQ>? NWqzPXp4OjRiaILz NFW4SmZKdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCFNUfCUE83KG2xdYPlJIx6dXCqIH7v[IUh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuOUDPxE1w M{faclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEexPFQ1Lz5{OUi3NVg1PDxxYU6=
HuH7 Mn7SRY51cX[rcnHsJIF{e2G7 NHrLclY{KGSjeYO= NE\CN3hCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMnGgbY5n\WO2ZXSgbY4hcHWvYX6gTJVJPyClZXzsd{Bi\nSncjCzJIRigXNiYomg[JVidC2pbH:gcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC55N{Og{txONg>? NFTUT3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC3OFc6PSd-M{CwO|Q4QTV:L3G+
MT4 NEjFUphEgXSxdH;4bYNqfHliYYPzZZk> Mne4OEB1dyB3IHThfZM> MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQhfG9iNTDkZZl{KGK7IHPlcIwufGm2ZYKg[4xwKGG|c3H5MEBESzVyIE2gOU43ODhizszNMi=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB5NEe5OUc,OzByN{S3PVU9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; 

PubMed: 12644839     


Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm).

Cyp-D / AIF / β-actin; 

PubMed: 29328412     


Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. *P<0.05, **P<0.01, ***P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor.

IL-8 / MCP-1; 

PubMed: 9602633     


Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity.

Actin / Smac; 

PubMed: 12427831     


Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release.

β-actin / FIPV-N; 

PubMed: 22546085     


Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.

12644839 29328412 9602633 12427831 22546085
Growth inhibition assay
NPC death; 

PubMed: 20181586     


Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).

20181586
IHC
Th22 / Th17 / Th1 / M1 / Th2; 

PubMed: 30916387     


(a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Tregs / Bregs / pDCs / M2; 

PubMed: 30916387     


(b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Ki 67 / Fas-FasL; 

PubMed: 30916387     


(c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

IFN-γ / TNF-α; 

PubMed: 10931158     


Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat.

WT-PCLS / FXRKO-PCLS; 

PubMed: 26482353     


Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).

30916387 10931158 26482353
Immunofluorescence
Human gingival fibroblast cells; 

PubMed: 27857765     


Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole.

Human gingival fibroblast cells; 

PubMed: 27857765     


Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts.

Calcein; 

PubMed: 23593196     


E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm).

Rhod-Dex / Calcein; 

PubMed: 20584761     


Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM).

cadherin-9 / vimentin; 

PubMed: 17668045     


To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.

27857765 23593196 20584761 17668045
In vivo Cyclosporin A is an immunosuppressive agent that is effective following either parenteral or oral administration in mice, rat and guinea pigs. [6], is clinically used in organ transplantation to prevent rejection. [7]

Protocol

Kinase Assay:[3]
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Phosphatase Assay:

Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described.
Cell Research:[3]
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  • Cell lines: Jurkat cells (clone J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1 hr
  • Method: Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.
    (Only for Reference)
Animal Research:[6]
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  • Animal Models: Rat
  • Dosages: 45 mg/kg
  • Administration: orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (83.15 mM)
Water Insoluble
Ethanol ''''100 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1202.61
Formula

C62H111N11O12

CAS No. 59865-13-3
Storage powder
in solvent
Synonyms Cyclosporine A, Cyclosporine, Ciclosporin, CsA
Smiles CCC1C(=O)N(CC(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)NC(C(=O)NC(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N1)C(C(C)CC=CC)O)C)C(C)C)C)CC(C)C)C)CC(C)C)C)C)C)CC(C)C)C)C(C)C)CC(C)C)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04989686 Not yet recruiting Other: Microsampling Immunosuppression Children''s Hospital of Philadelphia October 1 2021 --
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • Answer:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID