Cyclosporin A

For research use only.

Catalog No.S2286 Synonyms: Cyclosporine A, Cyclosporine, Ciclosporin, CsA

42 publications

Cyclosporin A  Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.

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Selleck's Cyclosporin A has been cited by 42 publications

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Biological Activity

Description Cyclosporin A (Cyclosporine A, Cyclosporine, Ciclosporin, CsA) is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.
Targets
calcineurin [1]
(Cell-free assay)
7 nM
In vitro

Cyclosporin A binds to the cyclophilin (immunophilin) in T cells[1] , forms a Cyclophilin-Cyclosporin A complex which the binds to and inhibits calcineurin. [2] Cyclosporin A inhibits calcineurin with IC50 of 7 nM[3], then blocks the nuclear translocation of NF-AT. [4] Cyclosporin A also affects mitochondria by preventing the MTP (mitochondrial permeability transition pore) from opening with an IC50 of 39 nM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells MkPkR5l1d3SxeHnjxsBie3OjeR?= MljFR5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFi0NmUh[2WubIOgZZN{\XO|ZXSgZZMhcW62cnHj[YxtfWyjcjDBWHAhdGW4ZXysJHREPTB;NEKg{txO MkTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3MEKwOVgoRjF7NUCyNFU5RC:jPh?=
mouse L929 cells NI\VbXFEgXSxdH;4bYPDqGG|c3H5 M{\5Z|Mh\GG7cx?= M1;IRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw6OjliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VWyCjc4PhfUwhUUN3ME2zNEDPxE1? NFWwPIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4OVE6PSd-MkW5PFUyQTV:L3G+
human Huh-Mono cells NYHRNGttS3m2b4TvfIlkyqCjc4PhfS=> NF3xR5k{KGSjeYO= M3zmWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2cC2Pb37vJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XoxvJygR2M2OD1{NDFOwG0> MmixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O|Y3RC:jPh?=
MDCK cells NIS2OlBEgXSxdH;4bYPDqGG|c3H5 MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEONIHPlcIx{NCCWQ{WwQVkvQSEQvF2= M2j3W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
human LCC-6 cells MofXR5l1d3SxeHnjxsBie3OjeR?= NHu0UYI4OiCq M1vVWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxESy14IHPlcIx{KGGodHXyJFczKGi{czygTWM2OD16LkOg{txO MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ|MkC0NFI9N2F-
human HeLa cells NVmycYNzS3m2b4TvfIlkyqCjc4PhfS=> NGXyVGxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{NCCWQ{WwQVgvOyEQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
african green monkey Vero cells M4LTUGZ2dmO2aX;uJIF{e2G7 NXX3fIxvOjRiaB?= MkDYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fCCQaXzlJJZqenW|IHnu[oVkfGWmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KF[ncn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygVm5CKHO7boTo[ZNqeyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRThizszN MofCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGyPFYoRjF7NEWxNlg3RC:jPh?=
human Huh-9-13 cells MX\DfZRwfG:6aXRCpIF{e2G7 NV7ySFhOOyCmYYnz MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYguQS1zMzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F697zOIFPDOVA:PyEQvF2= M3yxUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH6 cells NEO2U|REgXSxdH;4bYPDqGG|c3H5 MV[zJIRigXN? MlXVR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPiClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBESzVyPUeg{txO MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human Huh5-2 cells NWjUZVRGS3m2b4TvfIlkyqCjc4PhfS=> NHHucHo{KGSjeYO= NFfyc4FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWh3LUKgZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5NigSBuIFPDOVA:PiEQvF2= MojxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O|Y3RC:jPh?=
human HuH7 cells M1\FcGN6fG:2b4jpZ:Kh[XO|YYm= NVrHfoVUS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[Yxm[XOnIH;mJIxi[3SjdHWg[IVpgWS{b3flcoF{\SxiSVO1NF02NjhzIN88US=> NGq1OHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm0N|M6OCd-MkC5OFM{QTB:L3G+
human 2008 cells MVrGeY5kfGmxbjDhd5NigQ>? MX;Jcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD12Lke4JO69VQ>? M3m4WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{C3PFg1Lz5zOEewO|g5PDxxYU6=
human HeLa cells M2GxZ2Z2dmO2aX;uJIF{e2G7 M4KxcmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1zODDpcoZm[3SnZDDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNzDkZZl{97zOIGTDOVA:PC54IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
leukemia CEM cells MVHGeY5kfGmxbjDhd5NigQ>? NE\HUVFKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUHWvYX6gUWRTOSCSLVfsfYNweHKxdHXpckBCSkNiVILhcpNxd3K2ZYKgeZNqdmdibHX1b4VucWFiQ1XNJINmdGy|LDDJR|UxRTNwNDFOwG0> NVztc4Z{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKzOlE{QDdpPkGyN|YyOzh5PD;hQi=>
human Caco2 cells Mom2SpVv[3Srb36gZZN{[Xl? NWnMWIROdmirYnn0bY9vKG:oIGCt[5AhcW5iaIXtZY4hS2Glb{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGSrZ3;4bY4h\W[obIX4MEBKSzVyPUKuPUDPxE1? NH3vb3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O4NlQ2QCd-MkOzPFI1PTh:L3G+
human MDA435/LCC6MDR cells Ml3SR5l1d3SxeHnjxsBie3OjeR?= M1vSWlczcA>? NX3re3A1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiZYjwdoV{e2mwZzDNSHIh[W[2ZYKgO|IhcHK|78{MJGlEPTB;Mj64JO69VQ>? M17yXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
guinea pig ventricular myocytes M2HVXGZ2dmO2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKExvdInw[UBk[WylaYXtJINp[W6wZXygcYVie3W{ZXSgeZNqdmdid3jvcIUu[2WubDDwZZRkcCClbHHtdEBqdiCpdXnu[YEheGmpII\lcpRzcWO3bHHyJI16d2O7dHXzMEBKSzVyPUKg{txO MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd4MUCwNEc,OjJ5NkGwNFA9N2F-
KB/MDR cell M1XPVmZ2dmO2aX;uJIF{e2G7 NG\kN484OiCq M3PNWmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBMSi:PRGKgZ4VtdCCuaX7lJIFnfGW{IEeyJIhzKG:oIHTyeYch\Xiyb4P1doUtKEmFNUC9NU42KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MUm5NUc,OTV2OEG5PVE9N2F-
A2780/ADR cells MYnGeY5kfGmxbjDhd5NigQ>? NIXPc5dKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvKGW6cILld5Nm\CCrbjDBNlc5OC:DRGKgZ4VtdHNiYomgZ4Ft[2WrbjDBUUBie3Ojef-8kEBKSzVyPUGuOFEzPTRizszN M{DjTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEmwNFk1Lz5zN{i5NFA6PDxxYU6=
human K562/R7 cells NXrIO5I5TnWwY4Tpc44h[XO|YYm= MVKxJO69VQ>? MV23NkBp MXPQc5RmdnSrYYTpc44hd2ZiZH;4c5J2[mmlaX6tbY5lfWOnZDDjfZRwfG:6aXPpeJkh[WejaX7zeEBld3ixcoXibYNqdi2{ZYPpd5RidnRiaIXtZY4hUzV4Mj;SO{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ixcoXibYNqdiCLQ{WwJIF1KDFidV2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkeg{txO M2DscFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkO0NFQyLz5{NU[zOFA1OTxxYU6=
LLC-PK1 epithelial cells MlXxSpVv[3Srb36gZZN{[Xl? Mly3TY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYjDlfJBz\XO|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG|c3H589yNKEmFNUC9NE44KM7:TR?= MlnPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4OUmzPFkoRjF{Nkm5N|g6RC:jPh?=
mouse L5178 cells MlH0SpVv[3Srb36gZZN{[Xl? MXiyNEBucW6| MknNTY5pcWKrdHnvckBw\iCqdX3hckBCSkOEMT3t[YRq[XSnZDDybI9l[W2rbnWgNVI{KGWoZnz1fEBqdiCvb4Xz[UBNPTF5ODDj[YxteyCneIDy[ZN{cW6pIHj1cYFvKE2GUkGgZYZ1\XJiMkCgcYlveyCkeTDGRWNUKGGwYXz5d4l{NCCLQ{WwQVAvPjVizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2MUe0OUc,OjF|NEG3OFU9N2F-
KB cell NGjTOXpHfW6ldHnvckBie3OjeR?= MWrJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgT2Ih[2WubDDsbY5mKGGodHXyJFczKGhib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0xNjZizszN NU[wfo12RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFE6QTFpPkG1OFgyQTlzPD;hQi=>
mouse mammary carcinoma cell line EMT6/AR 1.0 NG[zWXlHfW6ldHnvckBie3OjeR?= M{fEbnJmfmW{c3HsJIVn\mWldDDvckB1cGViYXPjeY12dGG2aX;uJI9nKFt|SG2tJIRifW6xcoXibYNqdiCrbjDtc5V{\SCvYX3tZZJ6KGOjcnPpco9u[SClZXzsJIxqdmViRV3UOk9CWiBzLkCsJGlEPTB;MD60OEDPxE1? M1iyUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEm4OlcyLz5zMEC5PFY4OTxxYU6=
CK1 cells MnmySpVv[3Srb36gZZN{[Xl? NGrlTZRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJWFZvM{OgbY5n\WO2ZXSgbY4hS0tzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgO{Bl[Xm|LDDUR|UxRTBwMzFOwG0> M2PJN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
HEK293 cells NF\R[|dHfW6ldHnvckBie3OjeR?= M4LRSGlvcGmkaYTpc44hd2ZiT1HUVFFDOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNicILveIVqdi2vZXTpZZRm\CCyaYTheoF{fGG2aX6geZB1[Wun78{MJGtqRTBwMjFOwG0> NEPlXYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4O|k5Pid-MkK1PFc6QDZ:L3G+
human MDA435/LCC6MDR cells MULGeY5kfGmxbjDhd5NigQ>? M3LFeFUh\GG7cx?= NVyz[4VMVW:mdXzheIlwdiCxZjDQMYdxKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gbJVu[W5iTVTBOFM2N0yFQ{\NSHIh[2WubIOgZZN{\XO|ZXSgZZMhemW4ZYLzbY5oKHCjY3zpeIF5\WxicnXzbZN1[W6lZTDt[YF{fXKnZDDhd{Bk\WyuIIP1dpZqfmGuIHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XluIFXDOVA:OC5yM{Kg{txONg>? M170NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC Mn;iVJJwdGmoZYLheIlwdiCjc4PhfS=> NYn1eVlHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRm1ENCCLQ{WwQVEzKG6P MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ5OUOwPUc,OTV4N{mzNFk9N2F-
mouse T cells MnnDSpVv[3Srb36gZZN{[Xl? MkHSOEBl[Xm| MXHJcY12dm:|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBud3W|ZTDUJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidIfvJJdigSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKGGodHXyJFQh\GG7czDifUBOXFRiYYPzZZktKGmlNUC9NVEhdk1? MlPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OES5PFcoRjJ{OUi0PVg4RC:jPh?=
human splenocytes M37vdmZ2dmO2aX;uJIF{e2G7 NWXBZXYyPCCmYYnz NFrPW49KdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckB{eGynbn;jfZRmeyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFt|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCjZoTldkA1KGSjeYOgZpkh[Wyub3flcolkKG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44h[XO|YYmsJGlEPTB;MUCgcm0> NVLoOWMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlc5OzFpPkG5PFI4QDNzPD;hQi=>
human T-cell NFn6SHhHfW6ldHnvckBie3OjeR?= M2jjdWlvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUMYNmdGxicILv[JVkfGmxbjDv[kBtgW2yaH;rbY5mKEmOLUKsJGlEPTB;OD6xJI5O M1vtRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd3M{ezN|EoRjd3M{ezN|E9N2F-
human Jurkat cells M2G5NmZ2dmO2aX;uJIF{e2G7 MXOxJIg> NFj5[G9CdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKGi3bXHuJGp2emujdDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGGwdHmtR2Q{N0OGMkigZY51cWKxZImtbY5lfWOnZDDJUE0zKHC{b3T1Z5Rqd25idILlZZRm\CCob4KgNUBpeiCkZX\vdoUh[W62aT3DSFMwS0R{ODDhcpRq[m:meTDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMh[nliaX3teY5we3WycILld5Nqd25iYYPzZZktKEWFNUC9OUBvVQ>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|M{e5OUc,OTl7M{O3PVU9N2F-
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... Click to View More Cell Line Experimental Data

In vivo Cyclosporin A is an immunosuppressive agent that is effective following either parenteral or oral administration in mice, rat and guinea pigs. [6], is clinically used in organ transplantation to prevent rejection. [7]

Protocol

Kinase Assay:[3]
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Phosphatase Assay:

Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described.
Cell Research:[3]
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  • Cell lines: Jurkat cells (clone J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1 hr
  • Method: Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.
    (Only for Reference)
Animal Research:[6]
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  • Animal Models: Rat
  • Dosages: 45 mg/kg
  • Administration: orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (83.15 mM)
Water Insoluble
Ethanol ''100 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1202.61
Formula

C62H111N11O12

CAS No. 59865-13-3
Storage powder
in solvent
Synonyms Cyclosporine A, Cyclosporine, Ciclosporin, CsA
Smiles CCC1C(=O)N(CC(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)NC(C(=O)NC(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N(C(C(=O)N1)C(C(C)CC=CC)O)C)C(C)C)C)CC(C)C)C)CC(C)C)C)C)C)CC(C)C)C)C(C)C)CC(C)C)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • Answer:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

Tags: buy Cyclosporin A | Cyclosporin A supplier | purchase Cyclosporin A | Cyclosporin A cost | Cyclosporin A manufacturer | order Cyclosporin A | Cyclosporin A distributor
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID