For research use only.
Catalog No.S3956 Synonyms: ligustrazine
Molecular Weight(MW): 136.19
Tetramethylpyrazine (ligustrazine, TMP) is a natural compound isolated from Chinese herbal medicine Ligusticum wallichii (Chuan Xiong) with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.
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|Description||Tetramethylpyrazine (ligustrazine, TMP) is a natural compound isolated from Chinese herbal medicine Ligusticum wallichii (Chuan Xiong) with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.|
Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP could restrain mitochondrial ROS generation and upregulate the expression of PGC1, NRF1, and Tfam, which reflects mitochondrial biogenesis. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can exert antiapoptosis ability by inhibiting macrophage COX-2. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed.
|In vivo||Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation are significantly upregulated after Tetramethylpyrazine (TMP) pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. TMP is reported to possess a broad spectrum of pharmacological effects, such as antioxidant, anti-inflammatory, antifibrosis effects. Early pharmacokinetic research has determined the metabolism rate of TMP and verified its in-vivo short half-life of T1/2=2.89 h. TMP is found to protect ischemic brain damage, and promote cell proliferation and differentiation stimulated by ischemia.|
|In vitro||DMSO||27 mg/mL (198.25 mM)|
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
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Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04305184||Not yet recruiting||Drug: ASP0598|Drug: Matching Placebo||Chronic Tympanic Membrane Perforation||Astellas Pharma Global Development Inc.|Astellas Pharma Inc||May 2020||Phase 1|Phase 2|
|NCT04320316||Not yet recruiting||Drug: Crysvita (burosumab-twza) Treatment||Epidermal Nevus Syndrome||University of Alabama at Birmingham|Ultragenyx Pharmaceutical Inc||April 15 2020||Phase 4|
|NCT04263792||Recruiting||Drug: [18F]fluoropropyl-trimethoprim||Bacterial Infections||University of Pennsylvania||February 7 2020||Phase 1|
|NCT03993821||Active not recruiting||Drug: Burosumab||Cutaneous Skeletal Hypophosphatemia Syndrome (CSHS)|Epidermal Nevus Syndrome||Laura Tosi|Children''s National Research Institute|Ultragenyx Pharmaceutical Inc||July 1 2019||Early Phase 1|
|NCT03797729||Recruiting||Drug: Tirofiban|Drug: Normal saline||ST Elevation Myocardial Infarction||Shanghai Zhongshan Hospital||May 14 2019||Phase 4|
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