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Tetramethylpyrazine Immunology & Inflammation related chemical

Name: Tetramethylpyrazine
Brand: Selleck Chemicals
SKU: S3956
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S3956

Tetramethylpyrazine (ligustrazine, TMP) is a natural compound isolated from Chinese herbal medicine Ligusticum wallichii (Chuan Xiong) with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.

Chemical Structure

Molecular Weight: 136.19

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Quality Control

Batch: S395601 DMSO]100 mg/mL]false]Ethanol]100 mg/mL]false]Water]4 mg/mL]true Purity: 99.96%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

99.96

Related Targets	PD-1/PD-L1 CXCR STING AhR CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor
Other Immunology & Inflammation related Inhibitors	Cl-amidine Bestatin (Ubenimex) Bindarit (AF 2838) Tranilast Tempol Sinomenine GI254023X (GI4023) ATP Geniposidic acid CORM-3

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (734.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : 4 mg/mL (超声加热五分钟，水浴60℃)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Chemical Information, Storage & Stability

Molecular Weight	136.19	Formula	C₈H₁₂N₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1124-11-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Mechanism of Action

Targets/IC₅₀/Ki	PDE
In vitro	Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP could restrain mitochondrial ROS generation and upregulate the expression of PGC1, NRF1, and Tfam, which reflects mitochondrial biogenesis. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can exert antiapoptosis ability by inhibiting macrophage COX-2. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed.
In vivo	Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation are significantly upregulated after Tetramethylpyrazine (TMP) pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. TMP is reported to possess a broad spectrum of pharmacological effects, such as antioxidant, anti-inflammatory, antifibrosis effects. Early pharmacokinetic research has determined the metabolism rate of TMP and verified its in-vivo short half-life of T_1/2=2.89 h. TMP is found to protect ischemic brain damage, and promote cell proliferation and differentiation stimulated by ischemia.
References	[1] https://www.hindawi.com/journals/omcl/2016/2124638/ [2] https://pubmed.ncbi.nlm.nih.gov/24719552/ [3] https://pubmed.ncbi.nlm.nih.gov/9536520/ [4] https://pubmed.ncbi.nlm.nih.gov/22331136/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06122701	Not yet recruiting	Healthy	University Medicine Greifswald	November 20 2023	Not Applicable
NCT06025396	Completed	Cocaine Use Disorder\|Substance Use Disorders\|Healthy Volunteers	Tempero Bio Inc.\|National Institute on Drug Abuse (NIDA)	January 6 2023	Phase 1
NCT05509595	Active not recruiting	Fibrous Dysplasia Of Bone	National Institute of Dental and Craniofacial Research (NIDCR)\|National Institutes of Health Clinical Center (CC)	December 7 2022	Phase 2
NCT05357573	Active not recruiting	Tumor-Induced Osteomalacia (TIO)	Kyowa Kirin Co. Ltd.	September 7 2022	Phase 4

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