Name: Thymoquinone
Brand: Selleck Chemicals
SKU: S4761
Availability: InStock
Rating: 5 (4 reviews)

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Quality Control

Batch: Purity: 99.74%

99.74

Related Targets	PD-1/PD-L1 CXCR STING AhR CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor
Other Immunology & Inflammation related Inhibitors	Cl-amidine Bestatin (Ubenimex) Bindarit (AF 2838) Tempol Tranilast GI254023X (GI4023) Sinomenine Geniposidic acid CORM-3 Germacrone

Solubility

In vitro
Batch:

DMSO : 32 mg/mL (194.88 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.650mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.650mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	164.20	Formula	C₁₀H₁₂O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	490-91-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			SMILES	CC1=CC(=O)C(=CC1=O)C(C)C

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Thymoquinone (TQ) strongly inhibits the production of nitric oxide (NO) and repressed NO synthase (iNOS), tumor necrosis factor (TNF)-α, cyclooxygenase (COX)−2, interleukin (IL)−6, and IL-1β expression in LPS-activated RAW264.7 cells. TQ acts as a radical scavenger and thereby preserves the enzymatic activity of antioxidants like catalase, glutathione peroxidase, and glutathione-S-transferase. TQ abolishes the activity of IRAK1, but not IRAK4, thereby reducing the activity of downstream NF-κB and AP-1. Treatment with TQ significantly decreases cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment significantly decreases the level of phosphorylated p65 in the nucleus, which indicates the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreases the expression levels of VEGF, c-Myc and Bcl-2.
In vivo	Treatment of LPS/D-GalN–induced hepatitis and EtOH/HCl–induced gastritis mouse models with TQ significantly ameliorates disease symptoms.
References	[1] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316937/ [2] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038441/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-14)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07765745	RECRUITING	Radiographic Evaluation; Bone Density; Bone Width; Socket Preservation; Tooth Extraction; Thymoquinone	Kafrelsheikh University	2026-02-01
NCT07391384	NOT_YET_RECRUITING	Immediate Dental Implant Placement; Jumping Gap; Primary Stability of the Implants	shaimaa hamdy	2026-01-21
NCT07013058	COMPLETED	Metabolic Syndrome; Elevated Blood Pressure	Liaquat University of Medical & Health Sciences	2025-06-15
NCT07313111	COMPLETED	Diabetic Peripheral Neuropathy (DPN)	Hawler Medical University	2024-07-01	PHASE4
NCT06070311	UNKNOWN	Nasal Polyps; Chronic Sinusitis	Assiut University	2023-10
NCT07450807	COMPLETED	Changes in Human Gut Microbiome in Healthy Adults	Aysun Tekbaş	2024-04-02

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Thymoquinone Immunology & Inflammation related chemical

Chemical Structure

Molecular Weight: 164.20