Thymoquinone

Catalog No.S4761

For research use only.

Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.

Thymoquinone Chemical Structure

CAS No. 490-91-5

Selleck's Thymoquinone has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.
In vitro

Thymoquinone (TQ) strongly inhibits the production of nitric oxide (NO) and repressed NO synthase (iNOS), tumor necrosis factor (TNF)-α, cyclooxygenase (COX)−2, interleukin (IL)−6, and IL-1β expression in LPS-activated RAW264.7 cells. TQ acts as a radical scavenger and thereby preserves the enzymatic activity of antioxidants like catalase, glutathione peroxidase, and glutathione-S-transferase. TQ abolishes the activity of IRAK1, but not IRAK4, thereby reducing the activity of downstream NF-κB and AP-1[1]. Treatment with TQ significantly decreases cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment significantly decreases the level of phosphorylated p65 in the nucleus, which indicates the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreases the expression levels of VEGF, c-Myc and Bcl-2[2].

In vivo Treatment of LPS/D-GalN–induced hepatitis and EtOH/HCl–induced gastritis mouse models with TQ significantly ameliorates disease symptoms[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: RAW264.7 cells, peritoneal macrophages, and HEK293 cells
  • Concentrations: 6.25, 12.5, 25 μM
  • Incubation Time: 24 h
  • Method:

    The cell viability of RAW264.7 cells, peritoneal macrophages, and HEK293 cells during TQ exposure for 24 h is determined by a conventional MTT assay.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: an HCl/EtOH-induced gastritis mouse model (Male C57BL/6 and ICR mice)
  • Dosages: 5 and 25 mg/kg
  • Administration: Oral administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 32 mg/mL
(194.88 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 164.20
Formula

C10H12O2

CAS No. 490-91-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC(=O)C(=CC1=O)C(C)C

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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