Clone No.:2.4G2
The 2.4G2 monoclonal antibody (Clone: 2.4G2)reacts specifically with mouse CD16 (FcγRIII) and CD32 (FcγRII). It has also been reported to react non-specifically via its Fc domain to FcγRI. The 2.4G2 antibody is commonly used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen specific primary antibodies. For Fc receptor blocking of mouse samples in flow cytometry experiments, add 0.25 μg of anti-mouse CD16/32 antibody per 10⁶ cells in a total reaction volume of 100 μL.
| Featured Inhibitors | Y-27632 Dihydrochloride SB431542 CHIR-99021 (Laduviglusib) RMC-7977 RMC-6236 (Daraxonrasib) MRTX1133 MG132 Z-VAD-FMK VT3989 IAG933 |
|---|
| Molecular Weight | 150 kDa |
|---|---|
| Clone | 2.4G2 |
| Immunogen | BALB/c mouse macrophage cell line J774 |
| Isotype | Rat IgG2b,κ |
| Application | in vitro/vivo Fc receptor blocking |
| Source | CHO |
| Purification | protein A |
| Sterility | 0.2 μM filtered |
| Formulation | PBS buffer, pH 7.0 |
| Storage (From the date of receipt) |
Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles |
*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)
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