Home Immunology & Inflammation Immunology & Inflammation related inhibitor Sinomenine hydrochloride

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Sinomenine hydrochloride Immunology & Inflammation related inhibitor

Cat.No.S3758

Sinomenine (SN, Cucoline, Kukoline, Sabianine A), extracted from the Chinese medicinal plant, sinomenium acutum, is a potent anti-inflammatory and neuroprotective agent.
Sinomenine hydrochloride Immunology & Inflammation related inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 365.85

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Quality Control

Batch: S375801 DMSO]73 mg/mL]false]]]false]]]false Purity: 99.94%
99.94

Solubility

In vitro
Batch:

DMSO : 73 mg/mL (199.53 mM)
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In vivo
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Chemical Information, Storage & Stability

Molecular Weight 365.85 Formula

C19H23NO4.HCl

 Storage (From the date of receipt)
CAS No. 6080-33-7 Download SDF Storage of Stock Solutions

Synonyms Cucoline hydrochloride, Kukoline hydrochloride, Sabianine A hydrochloride Smiles CN1CCC23CC(=O)C(=CC2C1CC4=C3C(=C(C=C4)OC)O)OC.Cl

Mechanism of Action

In vitro
Sinomenine induces apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Sinomenine decreases the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. It has beneficial roles against several types of cancers, including breast cancer cells, esophageal carcinoma cells, hepatocellular carcinoma cells, gastric adenocarcinoma cells, lung cancer cells and colon carcinoma cells. Sinomenine treatment results in dose- and time-dependent growth inhibition in U87, and IC50 of SM ranged from 178 μM to 380 μM. Treatment of U251, U373, Hs683 and T98G with Sinomenine for 48 h obviously decreases cells viability, and IC50 are 342.7 μM, 430.2 μM, 189.6 μM and 270.3 μM, respectively. Sinomenine HCl (SH) activates an autophagy-mediated cell death pathway, as indicated by the accumulated microtubule-associated protein light chain 3B (LC3B)-II, triggers autophagic flux and enhances cell viability after pretreatment with autophagy inhibitors. It induces autophagy by inhibiting the Akt-mTOR pathway and activating the JNK pathway. Sinomenine HCl (SH) dose-dependently reduces the phosphorylation of p70S6K, 4E-BP1, Akt and mTOR, while enhancing the expression levels of autophagy marker proteins in vivo and in vitro. Sinomenine HCl dose-dependently augmentes ROS generation, which is accompanied by increased autophagy in both U87 and SF767 cells. SH may promote lysosomal biogenesis by triggering the nuclear translocation of TFEB via mTOR inhibition.
In vivo
Sinomenine has cardioprotective, anti-inflammatory activities and cancer chemopreventive property. It exhibits anti-glioma activity in vivo. Sinomenine is well tolerated by the recipient mice at therapeutic dose, and no significant cytotoxicity is accompanied. Sinomenine HCl (SH) initiates the autophagy-lysosome pathway in both in vitro and in vivo experiments.
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