Sinomenine hydrochloride

For research use only.

Catalog No.S3758 Synonyms: Cucoline hydrochloride, Kukoline hydrochloride, Sabianine A hydrochloride

Sinomenine hydrochloride Chemical Structure

Molecular Weight(MW): 365.85

Sinomenine (SN), extracted from the Chinese medicinal plant, sinomenium acutum, is a potent anti-inflammatory and neuroprotective agent.

Size Price Stock Quantity  
USD 117 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description Sinomenine (SN), extracted from the Chinese medicinal plant, sinomenium acutum, is a potent anti-inflammatory and neuroprotective agent.
In vitro

Sinomenine induces apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Sinomenine decreases the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. It has beneficial roles against several types of cancers, including breast cancer cells, esophageal carcinoma cells, hepatocellular carcinoma cells, gastric adenocarcinoma cells, lung cancer cells and colon carcinoma cells. Sinomenine treatment results in dose- and time-dependent growth inhibition in U87, and IC50 of SM ranged from 178 μM to 380 μM. Treatment of U251, U373, Hs683 and T98G with Sinomenine for 48 h obviously decreases cells viability, and IC50 are 342.7 μM, 430.2 μM, 189.6 μM and 270.3 μM, respectively[1]. Sinomenine HCl (SH) activates an autophagy-mediated cell death pathway, as indicated by the accumulated microtubule-associated protein light chain 3B (LC3B)-II, triggers autophagic flux and enhances cell viability after pretreatment with autophagy inhibitors. It induces autophagy by inhibiting the Akt-mTOR pathway and activating the JNK pathway. Sinomenine HCl (SH) dose-dependently reduces the phosphorylation of p70S6K, 4E-BP1, Akt and mTOR, while enhancing the expression levels of autophagy marker proteins in vivo and in vitro. Sinomenine HCl dose-dependently augmentes ROS generation, which is accompanied by increased autophagy in both U87 and SF767 cells. SH may promote lysosomal biogenesis by triggering the nuclear translocation of TFEB via mTOR inhibition[2].

In vivo Sinomenine has cardioprotective, anti-inflammatory activities and cancer chemopreventive property. It exhibits anti-glioma activity in vivo. Sinomenine is well tolerated by the recipient mice at therapeutic dose, and no significant cytotoxicity is accompanied[1]. Sinomenine HCl (SH) initiates the autophagy-lysosome pathway in both in vitro and in vivo experiments[2].


Cell Research:[1]
- Collapse
  • Cell lines: Human glioma cell lines (U87, U373, U251, Hs683 and T98G)
  • Concentrations: 0-1000 μM
  • Incubation Time: 24 h, 48 h and 72 h
  • Method: The cells are cultured in 96-well plates with a density of 5 × 103/well, and then treated with SM for the designated time (24-72 h). Following incubation, 10 μl MTT solution (5 g/l) is added to the medium in each well, and the microplate is incubated at 37 °C for 4 h. The absorbance is read in a microplate reader at 570 nm. Cytotoxicity is expressed as the percentage of cells surviving in relation to untreated cells.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: Nude mice (male, 5-week-old BALB/c)
  • Dosages: 50 mg/kg and 100 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 73 mg/mL (199.53 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 365.85


CAS No. 6080-33-7
Storage powder
in solvent
Synonyms Cucoline hydrochloride, Kukoline hydrochloride, Sabianine A hydrochloride
Smiles Cl.COC1=C(O)C2=C(CC3C4C=C(OC)C(=O)CC24CCN3C)C=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Sinomenine hydrochloride | Sinomenine hydrochloride supplier | purchase Sinomenine hydrochloride | Sinomenine hydrochloride cost | Sinomenine hydrochloride manufacturer | order Sinomenine hydrochloride | Sinomenine hydrochloride distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID