For research use only.
Catalog No.S3758 Synonyms: Cucoline hydrochloride, Kukoline hydrochloride, Sabianine A hydrochloride
Molecular Weight(MW): 365.85
Sinomenine (SN), extracted from the Chinese medicinal plant, sinomenium acutum, is a potent anti-inflammatory and neuroprotective agent.
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|Description||Sinomenine (SN), extracted from the Chinese medicinal plant, sinomenium acutum, is a potent anti-inflammatory and neuroprotective agent.|
Sinomenine induces apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Sinomenine decreases the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. It has beneficial roles against several types of cancers, including breast cancer cells, esophageal carcinoma cells, hepatocellular carcinoma cells, gastric adenocarcinoma cells, lung cancer cells and colon carcinoma cells. Sinomenine treatment results in dose- and time-dependent growth inhibition in U87, and IC50 of SM ranged from 178 μM to 380 μM. Treatment of U251, U373, Hs683 and T98G with Sinomenine for 48 h obviously decreases cells viability, and IC50 are 342.7 μM, 430.2 μM, 189.6 μM and 270.3 μM, respectively. Sinomenine HCl (SH) activates an autophagy-mediated cell death pathway, as indicated by the accumulated microtubule-associated protein light chain 3B (LC3B)-II, triggers autophagic flux and enhances cell viability after pretreatment with autophagy inhibitors. It induces autophagy by inhibiting the Akt-mTOR pathway and activating the JNK pathway. Sinomenine HCl (SH) dose-dependently reduces the phosphorylation of p70S6K, 4E-BP1, Akt and mTOR, while enhancing the expression levels of autophagy marker proteins in vivo and in vitro. Sinomenine HCl dose-dependently augmentes ROS generation, which is accompanied by increased autophagy in both U87 and SF767 cells. SH may promote lysosomal biogenesis by triggering the nuclear translocation of TFEB via mTOR inhibition.
|In vivo||Sinomenine has cardioprotective, anti-inflammatory activities and cancer chemopreventive property. It exhibits anti-glioma activity in vivo. Sinomenine is well tolerated by the recipient mice at therapeutic dose, and no significant cytotoxicity is accompanied. Sinomenine HCl (SH) initiates the autophagy-lysosome pathway in both in vitro and in vivo experiments.|
|In vitro||DMSO||73 mg/mL (199.53 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||Cucoline hydrochloride, Kukoline hydrochloride, Sabianine A hydrochloride|
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
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Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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