2x SYBR Green qPCR Master Mix

For research use only.

2x SYBR Green qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR.

2x SYBR Green qPCR Master Mix

Advantages

High Sensitivity: Lower Ct values ensure higher efficiency of amplification

Cost Effective: Offered at much discounted price compared to market price

Product Comparison

Higher amplification efficiency and stronger fluorescence signal

Using the same cDNA template and primers to amplify GAPDH, Selleck 2x SYBR Green QPCR Master Mix amplified faster with stronger fluorescence signal and better amplification efficiency than other brands.

Accurate quantitation and high stability

Using the same cDNA template and primers to amplify RPL37, Selleck 2x SYBR Green QPCR Master Mix shows accurate quantitation with high stability.

Higher specificity and sensitivity

Failure to amplify low-copy targets can be due to lack of sensitivity and/or specificity. Selleck 2x SYBR Green QPCR Master Mix uses a new type of hot-start enzyme which reduces non-specific amplification at low temperature, improving amplification of low-copy genes.

For example
Ct ValueGene Selleck Vazyme Yeasen Kapa Takara TIANGEN NEB TransGen
GAPDH (Human) 19.01 20.34 18.84 19.35 19.32 19.08 19.54 19.9
Actin (Human) 18.5 20.07 19 18.71 19.9 19.4 23.64 19.86
Vim (Human) 18.55 20.19 18.56 18.82 19.9 19.11 19.27 19.83
IL-6 (Human, low Copy) 29.03 29.55 32.54 27.56 35.12 33.31 33.02 29.54
GAPDH (Mouse) 20.2 21.37 20.39 21.34 20.83 20.67 21.01 21.85
GAPDH (Mouse) 20.2 21.37 20.39 21.34 20.83 20.67 21.01 21.85
RPL37 (Mouse) 18.76 19.69 18.77 18.77 19.3 19.14 19.3 19.86

Price Comparison

Description

2x SYBR Green qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

Applications

  • Dye-based quantitative PCR detection
  • Nucleic acid amplification and expression profiling
  • Quantitative genotyping studies
  • Genomics-related applications

Components

Component Cat#: B21202 Cat#: B21203
2x SYBR Green qPCR Master Mix * 5 mL
(for 200 reactions with 50 μl/rxn
for 500 reactions with 20 μl/rxn
for 1000 reactions with 10 μl/rxn)
25 mL
(for 1000 reactions with 50 μl/rxn
for 2500 reactions with 20 μl/rxn
for 5000 reactions with 10 μl/rxn)
50x ROX Dye (low conc.) 200 μL 1000 μL
50x ROX Dye (high conc.) 200 μL 1000 μL
* Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye

Storage

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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