Home Immunology & Inflammation Immunology & Inflammation related activator Phytohemagglutinin (PHA)

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Phytohemagglutinin (PHA) Immunology & Inflammation related activator

Cat.No.S6990

A potent mitogen from red kidney beans (Phaseolus vulgaris), phytohemagglutinin (PHA) stimulates lymphocyte proliferation and transforms them into large blast cells. It can suppress humoral antibody responses and enhance the survival of homografts by inhibiting immune rejection. It also triggers T-cell activation and apoptosis by upregulating pro-apoptotic proteins like Bax, leading to the activation of caspase-3.
Phytohemagglutinin (PHA) Immunology & Inflammation related activator Chemical Structure

Chemical Structure

Molecular Weight: N/A

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Quality Control

Batch: S699001 Water]100 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99%
99

Solubility

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Chemical Information, Storage & Stability

Molecular Weight N/A Formula

N/A

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 9008-97-3 -- Storage of Stock Solutions

Mechanism of Action

Targets/IC50/Ki
Bax
caspases-3
In vitro

Stimulation of human mononuclear leukocytes (MNL) by phytohemagglutinin (PHA) induces the expression of ChAT mRNA and potentiated ACh synthesis. This compound binds to the membranes of T-cells, stimulates metabolic activity, cell division, and involves inflammatory pathways.

In vivo

Phytohemagglutinin (PHA), a standard T cell mitogen, can inhibit both T- and B-cell tumors. This compound (100 μg; i.p.; daily for 2 days) inhibits A20 tumor growth in CB17-SCID mice.
References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05968170 Not yet recruiting
Graft Vs Host Disease|Graft-versus-host-disease|Acute Myeloid Leukemia|Acute Lymphoblastic Leukemia|Non-hematologic Malignancy|Hematologic Malignancy
Neena Kapoor M.D.|Children''s Hospital Los Angeles
 December 1 2023 Not Applicable
NCT05636995 Recruiting
Primary Hyperaldosteronism|Hypertensive Disorder of Pregnancy
Université de Sherbrooke|Foundation of the Stars
 January 10 2023 --

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