For research use only. Not for use in humans.

Ipilimumab (anti-CTLA-4)

Synonyms: MDX-010, BMS-734016 Cat.No.A2001 Purity:99%

Ipilimumab (anti-CTLA-4) is an immunomodulatory monoclonal antibody directed against the cell surface antigen CTLA-4 and also a type of immune checkpoint inhibitor. MW : 148 kD.

Ipilimumab (anti-CTLA-4) Chemical Structure

Click to purchase the isotype control of Ipilimumab (anti-CTLA-4)

19 Citations

17 Nobel Prize winners have used Selleck products

Shimon Sakaguchi

Nature Communications 2025,16, Article number:1325

David Baker

Dev Cell 2023, 58(20):2163-2180.e9.

David Julius

Cell 2017, 185-198.e16

Michael Houghton

Cell Chem Biol, 2020, 27(7):780-792.e5

Charles M. Rice

Cell 2018, 172(3):423-438.e25

Quality Control

Batch: Purity: 99% Protein concentration: 8.873mg/ml Endotoxin Level: ＜1EU/mg

Specificity

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1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description	Ipilimumab (anti-CTLA-4) is an immunomodulatory monoclonal antibody directed against the cell surface antigen CTLA-4 and also a type of immune checkpoint inhibitor. MW : 148 kD.
Features	Human IgG1
Targets/IC₅₀/Ki	CTLA-4 ^[1] (Cell-free assay)
In vitro	Pembrolizumab plus ipilimumab and nivolumab plus ipilimumab with chemotherapy have been approved by FDA for the first-line treatment of NSCLC patients.^[1]
Cell Research	For the intracellular cytokine analysis of TILs, cells from dissociated tumors in the density range of 1×10⁵ to 1×10⁶ cells were cultured in a 96-well U-bottom plate containing 10 µg/mL of pembrolizumab, 10 µg/mL of ipilimumab, soluble 50 ng/mL of anti-CD3, and 50 ng/mL of anti-CD28 for 24 h at 37°C, followed by the addition of brefeldin A and monensin. To measure interferon (IFN)-γ and tumor necrosis factor (TNF) secretion levels by CD8⁺ TILs according to PD-1 expression on anti-CD3 and anti-CD28 stimulation, the pre-incubation step without brefeldin A and monensin for 24 h was omitted. After 12 h, the cells were harvested and stained. Intracellular cytokines were stained after surface staining, fixation, and permeabilization with Foxp3 fixation/permeabilization solution.
In vivo	⁶⁴Cu-labeled ipilimumab may be used to noninvasively monitor CTLA-4-expressing tissues in vivo.^[2]
Animal Research	AnimalModels:Female athymic nude mice of human lung adenocarcinoma xenograft model Formulation:buffer-exchanged to PBS, and conjugated with DOTA for radiolabeling with ⁶⁴Cu (t1/2 = 12.7 h) Dosages:~1 mg/mL Administration:i.v.^[2]
References	[1] https://pubmed.ncbi.nlm.nih.gov/34907028/ [2] https://pubmed.ncbi.nlm.nih.gov/28388076/

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	477202-00-9
Isotype	Human IgG1
Source	CHO cells
Sterility	0.2 μM filtered
Formulation	PBS buffer, pH 7.2
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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Handling Instructions

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.