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Rheic Acid FTO inhibitor

Name: Rheic Acid
Brand: Selleck Chemicals
SKU: S2400
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S2400

Rhein (Monorhein, NSC 38629, Rheinic) is an anthraquinone compound enriched in the fresh rhizome of Rheum coreanum Nakai, showing anti-inflammation and antitumor activities. Rhein is an FTO (mRNA N⁶-methyladenine demethylase) inhibitor and also inhibits AlkB repair enzymes.

Chemical Structure

Molecular Weight: 284.22

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Quality Control

Batch: Purity: 99.73%

99.73

Related Targets	PD-1/PD-L1 CXCR STING AhR Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX
Other FTO Inhibitors	FB23-2 Dac51 FB23 Bisantrene (CS1)

Solubility

In vitro
Batch:

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.70mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.100mg/ml (0.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	284.22	Formula	C₁₅H₈O₆	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	478-43-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Rhein, Monorhein, NSC 38629, Rheinic Acid			Smiles	C1=CC2=C(C(=C1)O)C(=O)C3=C(C2=O)C=C(C=C3O)C(=O)O

Mechanism of Action

In vitro	Rhein at 35 μM can effectively inhibit the synthesis of IL-1β on human osteoarthritis synovium, as well as the action of this cytokine at the cartilage level, by reducing the number of chondrocyte IL-1R and inhibiting IL-1β induced NO production in chondrocytes and cartilage. Rhein also induces apoptosis in human promyelocytic leukemia cells (HL-60), characterized by caspase activation, poly(ADP)ribose polymerase (PARP) cleavage, and DNA fragmentation.
In vivo	Rhein is also effective in ameliorating diabetic renal pathological changes and attenuating hyperlipidemia in db/db mice with diabetic nephropathy with decreased transforming growth factor-beta1 (TGF-beta1) and fibronectin immunohistochemistry expression in renal tissue and reduced cholesterol (Chol), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and ApoE in plasma.
References	[1] https://pubmed.ncbi.nlm.nih.gov/9558181/ [2] https://pubmed.ncbi.nlm.nih.gov/9858439/ [3] https://pubmed.ncbi.nlm.nih.gov/14522581/ [4] https://pubmed.ncbi.nlm.nih.gov/19639539/

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