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Atractylenolide I Immunology & Inflammation related inhibitor

Name: Atractylenolide I
Brand: Selleck Chemicals
SKU: S8291
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S8291

Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.

Chemical Structure

Molecular Weight: 230.30

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.86%

99.86

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HepG2 cells	Function assay				Transactivation of p53 in human HepG2 cells expressing MDM2 promoter by transient transfection reporter assay
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	230.30	Formula	C₁₅H₁₈O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	73069-13-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC1=C2CC3C(=C)CCCC3(C=C2OC1=O)C

Solubility

In vitro
Batch:

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	6%DMSO 1 40%PEG300 2 6%Tween80 3 48%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	6.000mg/ml (26.05mM)	Taking the 1 mL working solution as an example, add 60 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 60 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 480 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Atractylenolide I decreases the TNF-α level in LPS-stimulated peritoneal macrophages in a dosedependent manner, IC50=23.1 μM. It also inhibits NO production in LPS-activated peritoneal macrophages, IC50=41 μM. The IC50 of this compound for inhibiting the activity of iNOS is 67.3 μM. It shows no marked effects against 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). This chemical is also reported previously to act on white blood cell membranes and TLR4 and its antiinflammatory activity is related to antagonizing TLR4 ^[1].
In vivo	Atractylenolide I has anti-inflammatory effects. The treatment of this compound significantly attenuates LPS-induced lung wet-to-dry weight ratio and MPO activity in LPS-induced acute lung injury mouse. It also significantly inhibits the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF, upregulates the production of IL-10 in BALF. This chemical protects mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation^[2].
References	https://pubmed.ncbi.nlm.nih.gov/17221938/ https://pubmed.ncbi.nlm.nih.gov/26297303/

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