Atractylenolide I

Catalog No.S8291

For research use only.

Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.

Atractylenolide I Chemical Structure

CAS No. 73069-13-3

Selleck's Atractylenolide I has been cited by 3 Publications

Purity & Quality Control

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.
In vitro

Atractylenolide I decreases the TNF-α level in LPS-stimulated peritoneal macrophages in a dosedependent manner, IC50=23.1 μM. It also inhibits NO production in LPS-activated peritoneal macrophages, IC50=41 μM. The IC50 of Atractylenolide I for inhibiting the activity of iNOS is 67.3 μM. It shows no marked effects against 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). Atractylenolide I is also reported previously to act on white blood cell membranes and TLR4 and its antiinflammatory activity is related to antagonizing TLR4 [1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells NFPn[WNHfW6ldHnvckBie3OjeR?= M1zwdXRz[W6|YXP0bZZifGmxbjDv[kBxPTNiaX6gbJVu[W5iSHXwS|Ih[2WubIOg[ZhxemW|c3nu[{BOTE1{IIDyc41wfGW{IHL5JJRz[W6|aXXueEB1emGwc3\lZ5Rqd25icnXwc5J1\XJiYYPzZZk> MlzaNlI{PjV5NU[=
In vivo Atractylenolide I has anti-inflammatory effects. The treatment of Atractylenolide I significantly attenuates LPS-induced lung wet-to-dry weight ratio and MPO activity in LPS-induced acute lung injury mouse. It also significantly inhibits the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF, upregulates the production of IL-10 in BALF. Atractylenolide I protects mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: peritoneal macrophages
  • Concentrations: 1-1000 μM
  • Incubation Time: 24 h
  • Method: 2 × 104 cells per well are plated into 96-well plates, cells/well to a final volume of 200 μL, incubated at 37 °C for 24 h, and given a fresh change of medium. The cells are treated with atractylenolide I (1-1000 μM) dissolved in DMSO, and with LPS (final concentration 10 μg/mL) alone or in combination with atractylenolide I (1–100 μM) dissolved in DMSO. This DMSO percentage allows the optimal solubilization of atractylenolide I in aqueous solution. The control and LPS wells receive the same amount of DMSO. After 24 h of incubation at 37 °C, 20 μL of MTT (5 mg/mL) is added to each well and 4 h later the cells are lysed with 150 μL DMSO. The plate is shaken for 10 min and 30 min later the optical densities (OD570) for atractylenolide I are compared with the OD of the control or LPS stimulated wells to assess the cytotoxicity.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: LPS-induced acute lung injury mouse
  • Dosages: 5, 10 and 20 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 46 mg/mL warmed
(199.73 mM)
Ethanol 46 mg/mL warmed
(199.73 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
6% DMSO+40% PEG 300+6% Tween 80+ddH2O
For best results, use promptly after mixing.

6mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 230.30
Formula

C15H18O2

CAS No. 73069-13-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C2CC3C(=C)CCCC3(C=C2OC1=O)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

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