2x SYBR Green qPCR Master Mix (High ROX)

For research use only.

2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR.

2x SYBR Green qPCR Master Mix (High ROX)

Advantages

Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples

High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection

Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods

Cost Effective: Offered at much discounted price compared to market price

Price Comparison

Description

2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with high concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT, StepOneTM, or StepOnePlusTM instruments with high sensitivity, specificity and reliability.

Two premixed versions (High ROX and Low ROX) are provided for the following qPCR instruments:
High ROX Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOneTM, StepOne PlusTM.
Low ROX Applied Biosystems 7500, 7500 Fast, ViiATM7; Stratagene MX4000TM, MX3005PTM, MX3000PTM, QuantiStudioTM 12K Flex.

Applications

  • Dye-based quantitative PCR detection
  • Nucleic acid amplification and expression profiling
  • Quantitative genotyping studies
  • Genomics-related applications

Components

Component Cat#: B21402 Cat#: B21403
2x SYBR Green qPCR Master Mix(High ROX) * 5 mL
(for 200 reactions with 50 μl/rxn
for 500 reactions with 20 μl/rxn
for 1000 reactions with 10 μl/rxn)
25 mL
(for 1000 reactions with 50 μl/rxn
for 2500 reactions with 20 μl/rxn
for 5000 reactions with 10 μl/rxn)
* Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye and ROX Dye (high concentration)

Storage

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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