Prednisolone (NSC-9900)

For research use only.

Catalog No.S1737

11 publications

Prednisolone (NSC-9900) Chemical Structure

CAS No. 50-24-8

Prednisolone (NSC-9900) is a synthetic glucocorticoid with anti-inflammatory and immunomodulating properties.

Selleck's Prednisolone (NSC-9900) has been cited by 11 publications

2 Customer Reviews

  • Combinations of EPZ-6438 with Prednisolone or Dexamethasone in DOHH2 EZH2 wild-type GCB lymphoma cell lines. All dose response plots were generated in Graphpad Prism and curves fitted to a four-parameter model with variable slope (2 biological replicates). Doses of EPZ-6438 ranged from 15.6-1000 nM, doses of Prednisolone ranged from 7.8–1000 nM, and doses of Dexamethasone ranged from
    0.8-100 nM. The potency of Prednisolone is increased with addition of EPZ-6438 in DOHH2 cells.

    PLoS One, 2014, 9(12):e111840.. Prednisolone (NSC-9900) purchased from Selleck.

    Inhibition of proliferation of CMV-specifi CD8+ T cells by everolimus. Isolated CD8+ T cells were labeled with CFSE and stimulated with CMVpp65 peptide in a MLPC in the presence of increasing doses of everolimus. After 6 days T cells were stained for CD8 expression and specifi city for the HLA-A2 restricted epitope pp65 (495-503) . The numbers show percentages of (B) tetramer-positive cells of total CD8+ T cells using fl ow cytometry. One representative histogram/dot blot out of six experiments is shown.

    Leuk Lymphoma, 2014, 55(5):1144-50. . Prednisolone (NSC-9900) purchased from Selleck.

Purity & Quality Control

Choose Selective Glucocorticoid Receptor Inhibitors

Biological Activity

Description Prednisolone (NSC-9900) is a synthetic glucocorticoid with anti-inflammatory and immunomodulating properties.
Targets
Glucocorticoid receptor [1]
()
In vitro

Prednisolone (50 mg/kg, im) given 15 min before LPS-attenuated production of NO2- and NO3- by neutrophils and suppresses LPS-stimulated mRNA for NOS II in rat neutrophils. [1] Prednisolone reduces joint swelling through a mechanism associated with a reduction in IL-1beta and IL-6 protein and mRNA expression levels in SCW-induced arthritis rats. [2] Prednisolone's anti-inflammatory effects can be mediated by reducing cellular adhesion molecule (CAM) expression. Prednisolone (0.75 mg/kg) reduces macrophages (-59%, -57%), CD4(+) T-cells (-50%, -60%), CD8(+) T-cells (-58%, -48%), and eosinophils (-36%, -25%) in quadriceps and soleus muscles, respectively. Prednisolone-treated mice also exhibits decreased vascular P-selectin (-82%) and ICAM-1 (-52%) expression and fewer L-selectin (-79%) and ICAM-1 (-57%) expressing mononuclear cells in quadriceps. Prednisolone reduces sarcolemmal damage and degeneration as well in Dystrophin-deficient, mdx mice. [3]

In vivo Prednisolone (5 mg/kg) causes alterations in diaphragmatic contractile properties and histological changes without fiber atrophy in rats. Prednisolone results in an increased number of diaphragmatic bundles in rats. [4] Prednisolone induces a significant decline of PL-pO2, while CoBF, CMs, CAPs, and ABRs revealed no change in guinea pigs. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (199.75 mM)
Water Insoluble
Ethanol '''10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 360.44
Formula

C21H28O5

CAS No. 50-24-8
Storage powder
in solvent
Synonyms N/A
Smiles CC12CC(C3C(C1CCC2(C(=O)CO)O)CCC4=CC(=O)C=CC34C)O

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04874610 Recruiting Drug: Methylprednisolone Pediatric Obesity|Asthma in Children Duke University August 16 2021 --
NCT04965636 Not yet recruiting Drug: Mepolizumab Hypereosinophilic Syndrome GlaxoSmithKline August 31 2021 Phase 3
NCT05001737 Not yet recruiting Drug: Emapalumab Macrophage Activation Syndrome|Secondary Hemophagocytic Lymphohistiocytosis|Still Disease|Systemic Lupus Erythematosus Swedish Orphan Biovitrum August 10 2021 Phase 3
NCT05006703 Recruiting Behavioral: Breathe4T Adolescent Behavior|Asthma University of Southampton|University of Bath|Aston University|University Hospital Southampton NHS Foundation Trust|Asthma UK June 22 2021 Not Applicable
NCT04797325 Not yet recruiting Drug: Vedolizumab|Drug: Prednisolone Immune-Mediated Colitis University of Copenhagen April 2021 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID