C-DIM12

C-DIM12 is a Nurr1 activator that stimulates Nurr1 mediated apoptosis axis in bladder cancer cells and tumors and inhibits NF-κB–dependent gene expression in glial cells.

C-DIM12 Chemical Structure

C-DIM12 Chemical Structure

CAS: 178946-89-9

Selleck's C-DIM12 has been cited by 2 publications

Purity & Quality Control

Batch: Purity: 99.79%
99.79

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Biological Activity

Description C-DIM12 is a Nurr1 activator that stimulates Nurr1 mediated apoptosis axis in bladder cancer cells and tumors and inhibits NF-κB–dependent gene expression in glial cells.
Targets
Nurr1 [2]
In vitro
In vitro C-DIM12 induces Nurr1 and DA gene expression in cell lines and primary neurons[3]. C-DIM12 suppresses astrocyte inflammatory signaling in vitro. C-DIM12 inhibits lipopolysaccharide (LPS)–induced expression of NF-κB-regulated genes in BV-2 microglia including nitric oxide synthase (NOS2), interleukin-6 (IL-6), and chemokine (C-C motif) ligand 2 (CCL2), and the effects were attenuated by Nurr1-RNA interference. Additionally, C-DIM12 decreased NF-κB activation in NF-κB–GFP (green fluorescent protein) reporter cells and enhanced nuclear translocation of Nurr1 primary microglia. C-DIM12 decreases lipopolysaccharide-induced p65 binding to the NOS2 promoter and concurrently enhanced binding of Nurr1 to the p65-binding site. C-DIM12 also stabilized binding of the Corepressor for Repressor Element 1 Silencing Transcription Factor (CoREST) and the Nuclear Receptor Corepressor 2 (NCOR2)[2].
Cell Research Cell lines HEK293 cells
Concentrations 100 μM
Incubation Time 24 h
Method NF-κB–GFP HEK cells are exposed to 30 ng/ml of TNFα in the presence of 100 μM C-DIM12 for up to 24 hours. C-DIM12 is efficient at blocking NF-κB–GFP expression in the NF-κB–GFP HEK cells after TNFα treatment, displaying a statistically significant reduction in total GFP fluorescence per cell.
In Vivo
In vivo C-DIM12 has the neuroprotective activity in MPTPp-treated mice[2].
Animal Research Animal Models Transgenic NF-κB/EGFP reporter mice (C57Bl/6 background)
Dosages 50 mg/kg
Administration oral administration

Chemical Information & Solubility

Molecular Weight 356.85 Formula

C23H17ClN2

CAS No. 178946-89-9 SDF Download C-DIM12 SDF
Smiles C1=CC=C2C(=C1)C(=CN2)C(C3=CC=C(C=C3)Cl)C4=CNC5=CC=CC=C54
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 71 mg/mL ( (198.96 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 71 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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