Sophoricoside

Catalog No.S3776

For research use only.

Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae) with numerous pharmacological effects, including anti- inflammatory, anti-cancer and immunosuppressive effects.

Sophoricoside Chemical Structure

CAS No. 152-95-4

Purity & Quality Control

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Biological Activity

Description Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae) with numerous pharmacological effects, including anti- inflammatory, anti-cancer and immunosuppressive effects.
In vitro

Treatment with sophoricoside at concentrations of 1–10 μM inhibited lipid accumulation in HepG2 cells in a dose-dependent manner. At the same concentration range, no effect on cell viability was observed in the MTT assay. Inhibition of lipogenesis was associated with the downregulation of SREBP-1a, SREBP-1c, SREBP-2 and their downstream target genes (FAS, ACC, HMGR). Sophoricoside has the capability to increase glucose uptake by C2C12 myotubes. It also effectively inhibited the activities of α-glucosidase and α-amylase in vitro[1].

In vivo Sophoricoside remarkably lowered postprandial hyperglycaemia in starch-loaded C57BL6/J mice[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HepG2 Cells
  • Concentrations: 0-75 μM
  • Incubation Time: 48 h
  • Method:

    HepG2 cells were cultured in a 24-well plate. After reaching confluence, the cells were incubated for 48 h in the presence of sophoricoside. Subsequently, the culture medium was removed and replaced by 500 μL of fresh culture medium containing 10% sterile filtered MTT. After 3 h, the insoluble formazan crystals were dissolved in 500 μL/well isopropanol and absorbance was measured at 570 nm, using the 630 nm reading as a reference.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: C57BL6/J mice
  • Dosages: 50, 100 and 200 mg/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 86 mg/mL
(198.89 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 432.38
Formula

C21H20O10

CAS No. 152-95-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)OC4C(C(C(C(O4)CO)O)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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