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Thymopentin

Name: Thymopentin
Brand: Selleck Chemicals
SKU: S3668
Rating: 5 (1 reviews)

Synonyms: TP5

Catalog No.S3668 Purity:99.87%

Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.

Thymopentin Chemical Structure

CAS No. 69558-55-0

Purity & Quality Control

Batch: Purity: 99.87%

99.87

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Biological Activity

Description	Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.

In vitro
In vitro	TP5 displays concentration-dependent inhibitory effects on the proliferation and colony formation of HL-60 cells. the decrease or even disappearance of AgNORs from nucleoli is observed in HL-60 cells after the treatment with TP5. The suppression induced by TP5 is accompanied by an accumulation of cell cycle in the G0/G1 phase. Moreover, TP5 significantly increases the NBT-reduction activity of HL-60 cells. TP5 has induced differentiation along the granulocytes lineage in HL-60 cells^[1]. TP5 increases the expression of CD4 and CD8 markers on the surface of human thymocytes.
Cell Research	Cell lines	HL-60 cells
	Concentrations	100–800 μmol/L
	Incubation Time	120 h
	Method	HL-60 cells are plated at a density of 1 × 10⁵ cells/mL, and cultured for 120h in a medium containing TP5 at various concentrations (100-800 μmol/L). To quantify the number of cells after treatment, cells are counted at different time points of incubation using a hemocytometer under a light microscope. Cell viability is determined by the Trypan blue exclusion assay. Viability is calculated as the ratio of living cells to the total cell population.

In Vivo
In vivo	TP5 is able to markedly stimulate the activity of immune system cells in healthy animals. It activates the production of a number of cytokines (IL1α, IL2, IL6, IL10, IFNγ), NO, Hsp70 and Hsp90 in mature cells of the peripheral immune system of mice (peritoneal macrophages and spleen lymphocytes)^[2].
Animal Research	Animal Models	NMRI mice
	Dosages	15 µg/100 g
	Administration	i.p.

References

Chemical Information & Solubility

Molecular Weight	679.77	Formula	C₃₀H₄₉N₉O₉
CAS No.	69558-55-0	SDF	Download Thymopentin SDF
Smiles	CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CCCN=C(N)N)N
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (147.1 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Clear solution	saline	100.000mg/ml (147.11mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	100.000mg/ml (147.11mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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