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Thymopentin

Catalog No.S3668 Synonyms: TP5

For research use only.

Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.

Thymopentin Chemical Structure

CAS No. 69558-55-0

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Biological Activity

Description Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.
In vitro

TP5 displays concentration-dependent inhibitory effects on the proliferation and colony formation of HL-60 cells. the decrease or even disappearance of AgNORs from nucleoli is observed in HL-60 cells after the treatment with TP5. The suppression induced by TP5 is accompanied by an accumulation of cell cycle in the G0/G1 phase. Moreover, TP5 significantly increases the NBT-reduction activity of HL-60 cells. TP5 has induced differentiation along the granulocytes lineage in HL-60 cells[1]. TP5 increases the expression of CD4 and CD8 markers on the surface of human thymocytes.

In vivo TP5 is able to markedly stimulate the activity of immune system cells in healthy animals. It activates the production of a number of cytokines (IL1α, IL2, IL6, IL10, IFNγ), NO, Hsp70 and Hsp90 in mature cells of the peripheral immune system of mice (peritoneal macrophages and spleen lymphocytes)[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HL-60 cells
  • Concentrations: 100–800 μmol/L
  • Incubation Time: 120 h
  • Method: HL-60 cells are plated at a density of 1 × 105 cells/mL, and cultured for 120h in a medium containing TP5 at various concentrations (100-800 μmol/L). To quantify the number of cells after treatment, cells are counted at different time points of incubation using a hemocytometer under a light microscope. Cell viability is determined by the Trypan blue exclusion assay. Viability is calculated as the ratio of living cells to the total cell population.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: NMRI mice
  • Dosages: 15 µg/100 g
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

 DMSO 100 mg/mL
(147.1 mM)
Water 100 mg/mL
(147.1 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 679.77
Formula

C30H49N9O9
CAS No. 69558-55-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CCCN=C(N)N)N

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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