In vivo Formulation Calculator

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent.
Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

User Guide: How to Design Your Experiment

Step 1: Define Experimental Parameters

Input your dosing requirements to calculate the total mass and volume needed.

Dosage (mg/kg): The amount of compound per unit of body weight.

Dosing Volume (μL): The volume injected per animal (e.g., 200μL for mice).

Number of Animals (Loss Allowance):
Recommended: An additional animal making an allowance for loss during the experiment

Step 2: Design Vehicle Composition

Define the percentage of each co-solvent. The calculator will automatically balance the remaining volume with ddH₂O.

Example: Entering 5% DMSO + 40% PEG300 will automatically set ddH2O to 55%.

How to Interpret and Use the Generated Protocol

Once you click "Calculate", the tool provides a three-part guide:

A. Working Concentration (mg/mL)

This is the final concentration of the compound in the syringe ready for injection.

Formula: Dosage (mg/kg) * Average Weight (kg) / Dosing Volume (mL)

Check: Ensure this concentration does not exceed the maximum solubility of the compound in your chosen vehicle.

B. Preparation of DMSO Master Liquid (The Stock)

To ensure complete dissolution, we recommend first dissolving the compound in 100% organic solvent (usually DMSO).

Action: Weigh the calculated mass of the drug and dissolve it entirely in the calculated volume of DMSO.

Why? Dissolving powder directly into a water-containing mixture is difficult and often leads to clumps. Starting with a high-concentration "Master Liquid" ensures homogeneity.

C. Preparation of Final In Vivo Formulation (The Dilution)

This section tells you exactly how much of each component to add to your Master Liquid.

Critical Order of Addition: Weigh the calculated mass of the drug and dissolve it entirely in the calculated volume of DMSO.

1.Take the DMSO Master Liquid.

2.Add Co-solvents (e.g., PEG300, Tween 80) sequentially.

3.Add Aqueous Phase (ddH2O/Saline) LAST.