Methylprednisolone
For research use only.
Licensed by Pfizer Catalog No.S1733 Synonyms: NSC-19987
3 publications
CAS No. 83-43-2
Methylprednisolone (NSC-19987) is a synthetic glucocorticoid receptor agonist, used to achieve prompt suppression of inflammation. Methylprednisolone activates ACE2 and reduces IL-6 levels, thus improves severe or critical COVID-19. Methylprednisolone markedly reduces autophagy and apoptosis.
Selleck's Methylprednisolone has been cited by 3 publications
Purity & Quality Control
Choose Selective Glucocorticoid Receptor Inhibitors
Biological Activity
| Description | Methylprednisolone (NSC-19987) is a synthetic glucocorticoid receptor agonist, used to achieve prompt suppression of inflammation. Methylprednisolone activates ACE2 and reduces IL-6 levels, thus improves severe or critical COVID-19. Methylprednisolone markedly reduces autophagy and apoptosis. | ||||||||||||||||
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| In vitro |
Methylprednisolone (2-10 mg/kg) markedly inhibits TNF production but does not affect serum levels of IL-10, while a high methylprednisolone dose (50 mg/kg) increases LPS-induced IL-10 levels. Methylprednisolone(from 0.01 to 100 mg/mL) also increases the biosynthesis of IL-10 by LPS-activated mouse peritoneal macrophages. [1] |
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| Cell Data |
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| In vivo | Methylprednisolone decreases RGC survival in rats with electrophysiologically diagnosed optic neuritis. Methylprednisolone decreases RGC survival by a nongenomic, calcium-dependent mechanism. Methylprednisolone-induced enhancement of RGC degeneration depends on calcium influx through voltage-gated calcium channels. [2] Methylprednisolone treatment leads to a significant decrease in the number of ED1-positive cells in both rostral and caudal stumps. Methylprednisolone treatment results in a significant reduction in tissue loss in both cord stumps at 2, 4 and 8 week post-injury. Methylprednisolone leads to a long-term reduction of ED1-positive cells and spinal tissue loss, reduced dieback of vestibulospinal fibres, and a transient sprouting of vestibulospinal fibres near the lesion at 1 and 2 weeks post-lesion. [3] Methylprednisolone at a dose of 30 mg/kg which has been shown to be effective in improving functional outcomes in rat SCI models, suppresses TNF-α expression and NF-kB activation. Methylprednisolone inhibition of NF-kB function is likely mediated by the induction of IkB, which traps NF-kB in inactive cytoplasmic complexes. [4] |
Protocol
Solubility (25°C)
| In vitro | DMSO | 75 mg/mL (200.28 mM) |
|---|---|---|
| Ethanol | 2 mg/mL (5.34 mM) | |
| Water | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 374.47 |
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| Formula | C22H30O5 |
| CAS No. | 83-43-2 |
| Storage |
powder in solvent |
| Synonyms | NSC-19987 |
| Smiles | CC1CC2C3CCC(C3(CC(C2C4(C1=CC(=O)C=C4)C)O)C)(C(=O)CO)O |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|---|
| NCT04560582 | Not yet recruiting | Procedure: Blood Draw | Kidney Transplant; Complications|Kidney Transplant Rejection|Kidney Transplant Failure | University of Minnesota | October 2020 | -- |
| NCT04477993 | Recruiting | Drug: Janus Kinase Inhibitor (ruxolitinib)|Other: Placebo | Severe Acute Respiratory Syndrome Coronavirus 2|SARS-CoV2 | Vanderson Geraldo Rocha|University of Sao Paulo General Hospital | July 17 2020 | Phase 2|Phase 3 |
| NCT04509219 | Recruiting | Drug: Methylprednisolone Injection | Mucocutaneous Lymph Node Syndrome | Chang Gung Memorial Hospital | April 15 2020 | Phase 1 |
| NCT04341038 | Recruiting | Drug: Tacrolimus|Drug: Methylprednisolone | COVID-19|Lung Injury | Hospital Universitari de Bellvitge|Institut d''Investigació Biomèdica de Bellvitge | April 1 2020 | Phase 3 |
| NCT04386603 | Completed | -- | Laparoscopic Surgery|Anesthesia | Shenzhen Second People''s Hospital|Shenzhen Third People''s Hospital | June 1 2019 | -- |
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