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N-Acetylcysteine amide MPO Inhibitor

Name: N-Acetylcysteine amide
Brand: Selleck Chemicals
SKU: S5804
Availability: InStock
Rating: 5 (15 reviews)

Cat.No.S5804

N-acetylcysteine amide is a membrane penetrating antioxidant with anti-inflamatory activity through regulation of activation of NF-κB and HIF-1α as well as modulation of ROS.

Chemical Structure

Molecular Weight: 162.21

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.28%

99.28

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Chemical Information, Storage & Stability

Molecular Weight	162.21	Formula	C₅H₁₀N₂O₂S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	38520-57-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(=O)NC(CS)C(=O)N

Solubility

In vitro
Batch:

DMSO : 32 mg/mL (197.27 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 32 mg/mL

Ethanol : 16 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	NF-κB ^[1] HIF-1α ^[1] ROS ^[1]
In vitro	The antioxidant N-acetylcysteine (NAC) blocks erastin-induced or RSL3-induced cell death in Nupr1^-/- mPDAC cells, an effect that is associated with decreased production or release of MDA, 8-OHdG, or HMGB1. NUPR1-knockdown PANC1 cells exhibits increased MDA, 8-OHdG, and HMGB1 release during ferroptotic cell death, which can be reversed by the addition of NAC.^[2]
In vivo	Combined n-acetylcysteine (NAC) and bone marrow-derived mesenchymal stem cells (BMSCs) therapy alleviates oxidative stress damage to the pancreas and inhibits the inflammatory response to a significantly greater extent than single therapy with either BMSCs or NAC.^[3]
References	[1] https://pubmed.ncbi.nlm.nih.gov/18160846/ [2] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843652/ [3] https://pubmed.ncbi.nlm.nih.gov/30660392/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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