Tempol
For research use only.
Catalog No.S2910 Synonyms: 4-Hydroxy-TEMPO
8 publications
CAS No. 2226-96-2
Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.
Purity & Quality Control
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Biological Activity
| Description | Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2. |
|---|---|
| In vitro |
Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. Tempol Exhibits radio-protective and anti-proliferative properties. Tempol inhibits oxygen-dependent radiation-induced damage. [1] Tempol induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner. [2] |
| In vivo | Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. [3] Tempol prevents cerebral vessel remodeling in hypertensive rats. [4] Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. [5] Tempol restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats. [6] |
Protocol
| Cell Research:[2] |
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| Animal Research:[3] |
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Solubility (25°C)
| In vitro | DMSO | 34 mg/mL (197.39 mM) |
|---|---|---|
| Water | 34 mg/mL (197.39 mM) | |
| Ethanol | 34 mg/mL (197.39 mM) | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing. |
30 mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 172.24 |
|---|---|
| Formula | C9H18NO2 |
| CAS No. | 2226-96-2 |
| Storage |
powder in solvent |
| Synonyms | 4-Hydroxy-TEMPO |
| Smiles | CC1(CC(CC(N1[O])(C)C)O)C |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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