Choose Your Country or Region

Tempol

Name: Tempol
Brand: Selleck Chemicals
SKU: S2910
Rating: 5 (14 reviews)

Synonyms: 4-Hydroxy-TEMPO

Catalog No.S2910 Purity:99.81%

Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.

Tempol Chemical Structure

CAS No. 2226-96-2

Purity & Quality Control

Batch: Purity: 99.81%

99.81

Tempol Related Products

Related Products	Cl-amidine (S,S)-TAPI-1 Bindarit Anti-mouse CD8α-InVivo Tranilast Sinomenine Geniposidic acid GI254023X CORM-3 Bestatin (Ubenimex) Acacetin Germacrone Oxymatrine Gastrodin Atractylenolide I Y-320 Sinomenine hydrochloride Thymoquinone Demethylzeylasteral (T-96) Lupeol Flufenamic acid Quercitrin Laquinimod Iguratimod Calycosin Sanguinarine Punicalagin Z-Guggulsterone Fenamic acid Tiragolumab (anti-TIGIT) Geniposide Thymol Jujuboside A PMX-53 6-Shogaol Isoliensinine Tiaprofenic acid Astragalin Linarin Isoliquiritin Coumarin Platycodin D Pipecolic acid Umbelliferone Thymopentin Salvianolic acid A VGX-1027 Sinigrin Isovitexin Buddlejasaponin IVb Lysionotin Liensinine Ginsenoside CK Lentinan Wogonoside Carnosol Madecassic acid Acetylharpagide Anwuligan Ginkgetin Eicosapentaenoic Acid (EPA) Tryptanthrin Pranlukast	Click to Expand
Related Compound Libraries	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Highly Selective Inhibitor Library	Click to Expand

Biological Activity

Description	Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.

In vitro
In vitro	Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. Tempol Exhibits radio-protective and anti-proliferative properties. Tempol inhibits oxygen-dependent radiation-induced damage. ^[1] Tempol induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner. ^[2]
Cell Research	Cell lines	HL60
	Concentrations	0.05–1.6 mM
	Incubation Time	96 h
	Method	The antiproliferative effect of Tempol is assessed by the MTT assay after 96 h exposure of the cells to the drug. Briefly, 100,000 cells/mL are seeded onto 96-well plates and allowed to grow for 24 h before treatment with different concentrations of Tempol (0.05–1.6 mM).

In Vivo
In vivo	Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. ^[3] Tempol prevents cerebral vessel remodeling in hypertensive rats. ^[4] Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. ^[5] Tempol restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats. ^[6]
Animal Research	Animal Models	Male Wistar rats
	Dosages	30 mg/kg
	Administration	bolus injection

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT04729595	Terminated	Covid19	Adamis Pharmaceuticals Corporation	September 1 2021	Phase 2\|Phase 3

References

+ Expand

Chemical Information & Solubility

Molecular Weight	172.24	Formula	C₉H₁₈NO₂
CAS No.	2226-96-2	SDF	Download Tempol SDF
Smiles	CC1(CC(CC(N1[O])(C)C)O)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 35 mg/mL ( (203.2 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 35 mg/mL

Ethanol : 35 mg/mL

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Clear solution	saline	35.000mg/ml (203.20mM)	Taking the 1 mL working solution as an example, add 35 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	34.000mg/ml (197.40mM)	Taking the 1 mL working solution as an example, add 34 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	Saline Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	30.000mg/ml (174.18mM)	Taking the 1 mL working solution as an example, add 30 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):