For research use only.

Catalog No.S2910 Synonyms: 4-Hydroxy-TEMPO

11 publications

Tempol Chemical Structure

CAS No. 2226-96-2

Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.

Selleck's Tempol has been cited by 11 publications

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Biological Activity

Description Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.
In vitro

Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. Tempol Exhibits radio-protective and anti-proliferative properties. Tempol inhibits oxygen-dependent radiation-induced damage. [1] Tempol induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner. [2]

In vivo Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. [3] Tempol prevents cerebral vessel remodeling in hypertensive rats. [4] Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. [5] Tempol restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats. [6]


Cell Research:[2]
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  • Cell lines: HL60
  • Concentrations: 0.05–1.6 mM
  • Incubation Time: 96 h
  • Method: The antiproliferative effect of Tempol is assessed by the MTT assay after 96 h exposure of the cells to the drug. Briefly, 100,000 cells/mL are seeded onto 96-well plates and allowed to grow for 24 h before treatment with different concentrations of Tempol (0.05–1.6 mM).
    (Only for Reference)
Animal Research:[3]
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  • Animal Models: Male Wistar rats
  • Dosages: 30 mg/kg
  • Administration: bolus injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 34 mg/mL (197.39 mM)
Water 34 mg/mL (197.39 mM)
Ethanol 34 mg/mL (197.39 mM)
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 172.24


CAS No. 2226-96-2
Storage powder
in solvent
Synonyms 4-Hydroxy-TEMPO
Smiles CC1(CC(CC(N1[O])(C)C)O)C

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID