Ligustrazine hydrochloride

Synonyms: Tetramethylpyrazine HCl, Tetrapyrazine HCl

Ligustrazine hydrochloride (Tetramethylpyrazine, Tetrapyrazine) is a chemical compound found in natto and in fermented cocoa beans with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.

Ligustrazine hydrochloride Chemical Structure

Ligustrazine hydrochloride Chemical Structure

CAS: 76494-51-4

Purity & Quality Control

Batch: S377501 DMSO] 34 mg/mL] false] ] ] false] ] ] false Purity: 99.65%
99.65

Ligustrazine hydrochloride Related Products

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description Ligustrazine hydrochloride (Tetramethylpyrazine, Tetrapyrazine) is a chemical compound found in natto and in fermented cocoa beans with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.
In vitro
In vitro Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP can affect the calcium influx, at least partly, by mediating the opening of potassium channel. TMP is also reported to have a direct effect on L-type calcium current, since it can reduce calcium transient in a dose-dependent manner when applied to rabbit ventricular myocyte. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed[1].
Cell Research Cell lines HL-60 cells
Concentrations 300 µg/mL
Incubation Time 24, 48, 60, 72 h
Method

Cell viability is determined by the MTT assay. HL-60 cells are treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively.

In Vivo
In vivo Tetramethylpyrazine (TMP) can stimulate NO production in pulmonary arteries of rat. Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation were significantly upregulated after TMP pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. It has a short in-vivo half-life (T1/2=2.89 h)[1].
Animal Research Animal Models A rat model of Parkinson
Dosages 20 mg/kg/d
Administration i.p.

Chemical Information & Solubility

Molecular Weight 172.66 Formula

C8H12N2.HCl

CAS No. 76494-51-4 SDF Download Ligustrazine hydrochloride SDF
Smiles CC1=C(N=C(C(=N1)C)C)C.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 34 mg/mL ( (196.91 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Ligustrazine hydrochloride | Ligustrazine hydrochloride supplier | purchase Ligustrazine hydrochloride | Ligustrazine hydrochloride cost | Ligustrazine hydrochloride manufacturer | order Ligustrazine hydrochloride | Ligustrazine hydrochloride distributor