Home Immunology & Inflammation Immunology & Inflammation related inhibitor Ganoderic acid A

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Ganoderic acid A Immunology & Inflammation related inhibitor

Cat.No.S4753

Ganoderic Acid A (GAA) is an active triangle compound in Ganoderma Lucidum. It can inhibit the JAK-STAT3 signal pathway and inhibit cell proliferation, vitality, and ROS. Ganoderic Acid A has analgesic, antioxidant, cytotoxicity, protecting liver and anti -tumor activity.
Ganoderic acid A Immunology & Inflammation related inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 516.67

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Quality Control

Batch: S475301 DMSO]100 mg/mL]false]Ethanol]100 mg/mL]false]Water]Insoluble]false Purity: 99.78%
99.78

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Bel7402 cells Cytotoxicity assay Cytotoxicity against human Bel7402 cells, IC50=7.25 μM
P388 cells Cytotoxicity assay Cytotoxicity against mouse P388 cells, IC50=7.25 μM
SGC7901 cells Cytotoxicity assay Cytotoxicity against human SGC7901 cells, IC50=7.25 μM
HepG2 cells Function assay 48 h Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay, IC50=38.7 μM
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (193.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble

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Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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%
% Tween 80
% ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 516.67 Formula

C30H44O7

 Storage (From the date of receipt)
CAS No. 81907-62-2 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CC(CC(=O)CC(C)C(=O)O)C1CC(C2(C1(CC(=O)C3=C2C(CC4C3(CCC(=O)C4(C)C)C)O)C)C)O

Mechanism of Action

In vitro
GAA exhibits antitumor activity on human osteosarcoma, lymphoma, meningioma and breast cancer cells through suppressing growth and invasive behavior and/or inducing apoptosis of cancer cells. GAA could also enhance chemosensitivity of HepG2 cells to Cisplatin. GA-A treatment induces caspase-dependent apoptotic cell death characterized by a dose-dependent increase in active caspases 9 and 3, up-regulation of pro-apoptotic BIM and BAX proteins, and a subsequent loss of mitochondrial membrane potential with release of cytochrome c. Lower doses of GA-A enhance HLA class II-mediated antigen presentation and CD4+ T cell recognition of lymphoma in vitro.
In vivo
GAA can significantly prolong the survival of EL4 syngeneic mice and decrease tumor metastasis to the liver, and enhance cell-mediated immune responses by attenuating myeloid-derived suppressor cells. GAA could undergo extensive metabolism, including reduction, oxidation, and hydroxylation phase I metabolism, and glucuronidation and sulfation phase II metabolism.
References

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