Echinocystic acid

Catalog No.S3837

For research use only.

Echinocystic acid (EA), a natural triterpone enriched in various herbs, displays a range of pharmacological activities including anti-inflammatory and antioxidant effects.

Echinocystic acid Chemical Structure

CAS No. 510-30-5

Purity & Quality Control

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description Echinocystic acid (EA), a natural triterpone enriched in various herbs, displays a range of pharmacological activities including anti-inflammatory and antioxidant effects.
In vitro

Echinocystic acid (EA) could induce apoptosis in human HepG2 cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. EA induces the truncation of Bid protein and reduction of Bcl-2 protein. EA also causes the loss of mitochondrial membrane potential (DWm) and cytochrome c release from mitochondria to cytosol. Moreover, EA could activate c-Jun NH2-terminal kinase (JNK) and p38 kinase, and JNK-specific inhibitor SP600125 and p38 kinase-specific inhibitor SB200235 could block serial molecular events of EA-induced apoptosis such as Bid truncation, Bcl-2 reduction, cytochrome c release, caspase activation, and DNA fragmentation in HepG2 cells. EA inhibits HepG2 cell proliferation in a dose-dependent manner with an IC50 value of 45.4 μM at 24h treatment[1].

In vivo Administration of Echinocystic acid (EA) is found to improve the maximum stress and Young’s modulus of femur in OVX rats. Micro-computed tomography analysis reveals that EA could improve the trabecular architecture, as shown by increasing the BV/TV, Tb.N, and Tb.Th in OVX rats. However, EA does not affect the body weight and uterine weight. EA has been shown to display an anti-inflammatory effect in different models of chronic inflammation in mice through the down-regulation of pro-inflammatory cytokines such as IL-1β, IL-18, TNF-α[2]. EA attenuates reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus[3].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HepG2 cells
  • Concentrations: 0, 15, 30, 45, 60, 100 μM
  • Incubation Time: 24h
  • Method: The cell survival rate is measured by a MTT assay. HepG2 cells (2 ×104/well) are incubated with different concentrations of EA in 96-well plates for 24h. MTT solution (5 mg/ml) is then added to each well and incubated for another 4h. The resulting MTT-formazan product is dissolved by the addition of 0.04N HCl-isopropanol solutions. Absorbance is measured at 595 nm with a microplate reader. The cell viability is expressed as the optical density ratio of the treatment to control.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: female ovariectomy (OVX) Sprague–Dawley rats
  • Dosages: 1, 5 or 15 mg/kg/day
  • Administration: ig
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 94 mg/mL
(198.85 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 472.70


CAS No. 510-30-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1(CCC2(C(C1)C3=CCC4C5(CCC(C(C5CCC4(C3(CC2O)C)C)(C)C)O)C)C(=O)O)C

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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