CRISPR/Cas9

Identification of a Cryptic Binding Site in CRISPR-Cas9 for Targeted Inhibition

54 views | Aug 31 2023

This study discovered a hidden ligand binding site within the carboxyl-terminal domain (CTD) of CRISPR-Cas9, revealing how the binding of small molecules induces structural changes that inhibit the function of Cas9. [Read the Full Post]

5-Oxoproline Enhances 4-Hydroxytamoxifen-induced Cytotoxicity by Increasing Oxidative Stress in MCF-7 Breast Cancer Cells

0 views | Jul 01 2023

In conclusion, the study demonstrates that 5-oxoproline enhances the cytotoxic effects of 4-hydroxytamoxifen in estrogen receptor-positive breast cancer cells under normal glucose conditions, suggesting the potential utility of monocarboxylate transporter inhibitors as adjunctive therapeutic agents. [Read the Full Post]

Generation of a Live Attenuated Influenza A Vaccine Using Chemical-Triggered Intein

69 views | Jul 01 2023

A novel method using 4-hydroxytamoxifen (4-HT)-dependent intein insertion into the polymerase acidic (PA) protein of influenza A virus generates recombinant viruses that demonstrate excellent replication characteristics and induce robust immunity, offering potential for the development of a more effective live attenuated influenza vaccine and possibly vaccines against other pathogens as well. [Read the Full Post]

Bridging Gaps in HDR Improvement: The Role of MAD2L2, SCAI, and SCR7

91 views | Jun 25 2023

This study demonstrated that MAD2L2 knockdown and SCR7 treatment significantly improved homology-directed repair (HDR) efficiency during CRISPR/Cas-mediated genome editing, offering valuable insights for enhancing precise correction in gene editing. [Read the Full Post]

Kv1.3 blockade by ShK186 modulates CD4+ effector memory T-cell activity of patients with Granulomatosis with polyangiitis

49 views | May 19 2023

The study suggests that Kv1.3 blockade using ShK-186 may be a potential treatment strategy for GPA by modulating the activity of CD4+TEM cells and reducing the production of pro-inflammatory cytokines, with less harmful side effects than current immunosuppressive regimens. [Read the Full Post]

Methyltransferase K-D-K-E motif influences the intercellular transmission of Newcastle disease virus

176 views | Apr 05 2023

This research demonstrates that mutations in the K-D-K-E methyltransferase motif restrict the cell-to-cell spread of Newcastle disease virus via tunnelling nanotubes. [Read the Full Post]

Monitoring paxillin in astrocytes reveals the significance of the adhesion GPCR VLGR1/ADGRV1 for focal adhesion assembly

97 views | Apr 04 2023

VLGR1 is a vital component of focal adhesions and regulates cell migration by controlling focal adhesion turnover during their assembly, and its dysfunctions may contribute to the pathogenesis of Usher syndrome, epilepsy, and other related diseases. [Read the Full Post]

Chloroquine-Induced DNA Damage Synergizes with Nonhomologous End Joining Inhibition to Cause Ovarian Cancer Cell Cytotoxicity

60 views | Aug 11 2022

María Ovejero-Sánchez et al. found that NHEJ inhibition increased the persistence of γH2AX foci after CQ-induced DNA damage, revealing an essential role of this pathway in the repair of the lesions. [Read the Full Post]

Lung Cancer Targeted Chemoradiotherapy via Dual-Stimuli Responsive Biodegradable Core-Shell Nanoparticles

65 views | Aug 10 2022

Roshni Iyer et al. found that E-DSNPs displayed very high uptake by lung cancer cells compared to healthy lung epithelial cells. [Read the Full Post]

Defining Endocytic Pathways of Fucoidan-Coated PIBCA Nanoparticles from the Design of their Surface Architecture

635 views | Mar 19 2022

M C B Lira-Nogueira et al. found that internalization pathways of PIBCA nanoparticles by J774A.1 macrophages could be determined by nanoparticle fucoidan surface composition and architecture. [Read the Full Post]