NF-κB
Signaling Pathway Map
Research Area
- Inhibitory Selectivity
- Solubility
| Catalog No. | Product Name | Solubility(25°C) | ||
|---|---|---|---|---|
| Water | DMSO | Alcohol | ||
| S4902 | QNZ (EVP4593) | <1 mg/mL | 5 mg/mL | <1 mg/mL |
| S1848 | Curcumin | <1 mg/mL | 73 mg/mL | <1 mg/mL |
| S4073 | Sodium 4-Aminosalicylate | 42 mg/mL | 42 mg/mL | <1 mg/mL |
| S7351 | JSH-23 | <1 mg/mL | 48 mg/mL | 20 mg/mL |
| S7414 | Caffeic Acid Phenethyl Ester | <1 mg/mL | 57 mg/mL | 57 mg/mL |
| S8642 | GSK'963 | <1 mg/mL | 46 mg/mL | 46 mg/mL |
| S3867 | (E)-Cardamoni | -1 mg/mL | 54 mg/mL | -1 mg/mL |
| S3874 | Curcumenol | -1 mg/mL | 46 mg/mL | -1 mg/mL |
| S3633 | Pyrrolidinedithiocarbamate ammonium | 32 mg/mL | 32 mg/mL | 32 mg/mL |
| S3137 | Sodium salicylate | 32 mg/mL | 32 mg/mL | <1 mg/mL |
| S7672 | Omaveloxolone (RTA-408) | <1 mg/mL | 100 mg/mL | 20 mg/mL |
| S8484 | GSK2982772 | <1 mg/mL | 75 mg/mL | 30 mg/mL |
| S8261 | GSK583 | <1 mg/mL | 79 mg/mL | 28 mg/mL |
| S2261 | Andrographolide | <1 mg/mL | 70 mg/mL | <1 mg/mL |
| S2382 | Evodiamine | <1 mg/mL | <1 mg/mL | <1 mg/mL |
| S2313 | Indole-3-carbinol | 7 mg/mL | 29 mg/mL | 29 mg/mL |
| S7273 | SC75741 | <1 mg/mL | 35 mg/mL | <1 mg/mL |
| S2321 | Magnolol | <1 mg/mL | 53 mg/mL | 53 mg/mL |
| S2341 | (-)-Parthenolide | <1 mg/mL | 49 mg/mL | 49 mg/mL |
| S3604 | Triptolide (PG490) | <1 mg/mL | 72 mg/mL | <1 mg/mL |
| S8483 | CBL0137 (CBL-0137) | 24 mg/mL | 38 mg/mL | <1 mg/mL |
- NF-κB Inhibitors (22)
- New NF-κB Products
| Catalog No. | Information | Product Use Citations | Product Validations |
|---|---|---|---|
| S4902 |
QNZ (EVP4593)QNZ (EVP4593) shows potent inhibitory activity toward both NF-κB activation and TNF-α production with IC50 of 11 nM and 7 nM in Jurkat T cells, respectively. |
NF-κB partly mediated resveratrol (Res) inhibition on PKD inflammation. (A) Expression of p-p65, p65, p105, p50, TNF-α, MCP-1 and CFB in QNZ-treated OX161 cells. (B) OX161 cells were pretreated with QNZ for 2 h and followed by the treatment of resveratrol for 46 h. The expression of p-p65, p65, p105, p50, TNF-α, MCP-1 and CFB were evaluated by western blot. (C) MCP-1 or CFB of QNZ-and/or resveratroltreated OX161 cell supernatants were measured by ELISA. One representative of three independent experiments is shown.
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| S1848 |
CurcuminCurcumin is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase（IC50~25 μM） and Histone deacetylase; activates Nrf2 pathway and supresses the activation of transcription factor NF-κB. |
Effect of NC1, NC1-CUR, NC2 and NC2-CUR on SH-SY5Y cell viability. The cells were treated for 24 h with different dilutions(1:1, 1:2 and 1:4) of empty nanocapsules (NC1 and NC2) and nanocapsules containing curcumin (NC1-CUR and NC2-CUR) followed by measurement of cell viability by MTT reduction assay (panel A) and cell toxicity by LDH release assay (panel B). Data after normalization to vehicle-treated cells (100%, MTT assay) or to total LDH release (TritonX100-treated cells, 100%) are presented as a mean±SEM from 3-8 independent experiments with five replicates. *p < 0.05, **p < 0.01 and ***p<0.001 versus vehicle-treated cells; &&&p<0.001 NC1 versus NC1-CUR.
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| S4073 |
Sodium 4-AminosalicylateSodium 4-Aminosalicylate is an antibiotic used to treat tuberculosis via NF-κB inhibition and free radical scavenging. |
A. Raw264.7 cells were pretreated with NAC (1 mM), SB202190 (10 μM) and Sodium 4-aminosalicylate (10 μM), respectively, for 30 min prior to 10 μg/ml ConA treatment for 24 h. Cell viability was measured by an MTT assay and ROS production detected by H2DCF-DA.
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| S7351 |
JSH-23JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line. |
CD10+GPR77+ CAFs were transduced without (-) or with GFP-shRNA or p65 shRNA or pretreated with inhibitors of NF-kB nuclear translocation. Quantitation of the percentage of CD10+GPR77+ CAFs (D) and the IL-6 and IL-8 levels (E) were shown.
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| S7414 |
Caffeic Acid Phenethyl EsterCaffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities. |
The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.
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| S8642New |
GSK'963GSK'963 is a chiral small-molecule inhibitor of RIP1 kinase with an IC50 of 29 nM in FP binding assays. It is >10 000-fold selective for RIP1 over 339 other kinases. |
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| S3867New |
(E)-CardamoniCardamonin is a naturally occurring chalcone with strong anti-inflammatory activity. It is a novel TRPA1 antagonist with IC50 of 454 nM and also a NF-kB inhibitor. |
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| S3874New |
CurcumenolCurcumenol, a sesquiterpene isolated from Curcuma zedoaria, is known to possess a variety of health and medicinal values which includes neuroprotection, anti-inflammatory, anti-tumor and hepatoprotective activities. It inhibits NF-κB activation by suppressing the nuclear translocation of the NF-κB p65 subunit and blocking IκBα phosphorylation and degradation. |
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| S3633New |
Pyrrolidinedithiocarbamate ammoniumAmmonium pyrrolidine dithiocarbamate (PDTC) is a potent nuclear factor-κB (NF-κB) inhibitor that inhibits IκB phosphorylation, blocks NF-κB translocation to the nucleus and reduces the expression of downstream cytokines. |
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| S3137 |
Sodium salicylateSodium salicylate is used in medicine as an analgesic and antipyretic. |
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| S7672 |
Omaveloxolone (RTA-408)Omaveloxolone (RTA-408) is a synthetic triterpenoid that activates the cytoprotective transcription factor Nrf2 and inhibits NF-κB signaling. Phase 2. |
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| S8484 |
GSK2982772GSK2982772 is an ATP competitive receptor-interacting protein-1 (RIP1) kinase inhibitor with the IC50 value of 1 nM. It has exquisite kinase specificity and excellent activity in blocking many TNF-dependent cellular responses. |
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| S8261 |
GSK583GSK583 is a highly potent and selective inhibitor of RIP2 kinase with an IC50 of 5 nM. |
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| S2261 |
AndrographolideAndrographolide is a labdane diterpenoid that is the main bioactive component of the medicinal plant Andrographis paniculata. |
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| S2382 |
EvodiamineEvodiamine (Isoevodiamine), an alkaloid extract from the fruit of Evodiae Fructus exhibits antitumor activities against the human tumor cells. It is shown to inhibit NF-kB activation through suppression of IkB kinase activity. |
Evodiamine ameliorates PDGF‑BB‑induced oxidative stress in VSMCs. VSMCs were pretreated with 0.1 or 0.5 μM evodiamine for 24 h, and then stimulated with 10 ng/ml PDGF‑BB for 1 h. (A) ROS detection using 2' 7' dichlorofluorescin diacetate staining. Magnification, x200. (B) Quantitative data of five independent experiments, expressed as the fold increase, compared with the control. (C‑F) mRNA expression levels of antioxidant genes were assessed using reverse transcription‑quantitative polymerase chain reaction analysis. Data are presented as the mean+ standard deviation of three independent experiments. *P<0.05 and **P<0.01, compared with the control group; ##P<0.01, compared with the PDGF‑BB‑stimulated group. Evo, evodiamine; PDGF‑BB, platelet‑derived growth factor‑BB; VSMCs, vascular smooth muscle cells; ROS, reactive oxygen species; HO‑1, heme oxygenase‑1; GPx‑1, glutathione peroxidase 1; SOD, superoxide dismutase.
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| S2313 |
Indole-3-carbinolIndole-3-carbinol is produced by the breakdown of the glucosinolate glucobrassicin, which can be found at relatively high levels in cruciferous vegetables. Indole-3-carbinol suppresses NF-κB and IκBα kinase activation. |
CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.
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| S7273 |
SC75741SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. |
Immunofluorescence of NF-κB (p65) (200×). SK-OV-3 and A2780S cells were treated with different compounds (vehicle, 5 μM JSH-23, 5 μM SC75741, 5 μM CAPE, 4 μM MIL, 4 μM MIL combined with pre-treated 5 μM JSH-23, 4 μM MIL combined with pre-treated 5 μM SC75741, 4 μM MIL combined with pre-treated 5 μM CAPE) for 48 h. NF-κB(p65) was detected by a fluorescence microscopy. The nuclei were stained with Hoechst 33258.
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| S2321 |
MagnololMagnolol is a bioactive lignin found in the bark of the Houpu magnolia (Magnolia officinalis) which shows antifungal properties. It can block TNF-α-induced NF-KB activation. |
Magnolol reduces RNV areas and avascular areas in OIR mice. Mice retinas from OIR group (a, b) and OIR + Magnolol (25 mg/kg) group (c, d) were harvested at P17 and subjected to wholemount immunostaining with IB4 (red), showing neovascular areas (green) and avascular areas (yellow).
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| S2341 |
(-)-Parthenolide(-)-Parthenolide, an inhibitor of the Nuclear Factor-κB Pathway, specifically depletes HDAC1 protein without affecting other class I/II HDACs; Also promotes the ubiquitination of MDM2 and activates p53 cellular functions. |
-Parthenolide-S234101W0220161110.gif)
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G. To evaluate effects of IKBKE/TBK1 inhibition on NF-κB signaling in Ewing, TC32 cells were incubated with CYT387 for six hours prior to stimulation with TNF-α (30 ng/mL). IκBα degradation was measured by harvesting TC32 cells thirty minutes after stimulation with TNF-α. TNF-α stimulation resulted in degradation of IκBα, and this effect was attenuated with CYT387 treatment. Parthenolide, an inhibitor of IκBα phosphorylation was used as a positive control. Similar effects of CYT387 activity were seen in HEK-293T cells which also express IKBKΕ. Nuclear extracts were prepared from TC32 cells harvested following forty-five minutes of TNF-α stimulation. Treatment with CYT387 resulted in decreased nuclear localization of NF-κB family proteins RelA/p65 and c-Rel. There was a modest impairment of p50 nuclear localization as compared to parthenolide and DMSO controls and no change in p52 nuclear localization. RelB (not shown) is not expressed in TC32 cells |
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| S3604 |
Triptolide (PG490)Triptolide is a diterpene triepoxide, immunosuppresive agent extracted from the Chinese herb Tripterygium wilfordii. It functions as a NF-κB inhibitor with dual actions by disruption of p65/CBP interaction and by reduction of p65 protein. |
Treatment with triptolide selectively induces apoptosis of cultured and primary CD19+ CLL cells. A. Percent apoptosis (Annexin V-FITC and PI staining) in Mec-1, Mec-2 and WAC3-CD5+ cells exposed to the indicated doses of triptolide for 48 hrs; bars indicate standard deviation. B. Percent apoptosis of CD19+ normal and primary CLL (both high and low risk) cells exposed to the indicated doses of triptolide for 48 hrs; bars indicate standard deviation. C. Percent of WaC3-CD5+ and Mec-1 cells in different phases of the cell cycle (G0/G1, S and G2/M) exposed to the indicated doses of triptolide for 24 hrs. D. Immunoblot analyses of HSF1, HSP70, cleaved caspase-3 and β-actin obtained from the cell lysates of CD19+ primary CLL cell samples treated with the indicated doses of triptolide for 24 hrs.
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| S4712 |
DiethylmaleateDiethylmaleate is the diethyl ester of maleic acid and a glutathione-depleting compound that inhibits NF-kB. |
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| S8483New |
CBL0137 (CBL-0137)CBL0137(CBL-0137) activates p53 and inhibits NF-kB with EC50s of 0.37 μM and 0.47 μM in the cell-based p53 and NF-kB reporter assays, respectively. It also inhibits histone chaperone FACT (facilitates chromatin transcription complex). |
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