Scutellarin

Catalog No.S3810 Synonyms: Breviscapine, Breviscapin, Scutellarein-7-glucuronide

For research use only.

Scutellarin (Breviscapine, Breviscapin, Scutellarein-7-glucuronide), the major active principal flavonoids extracted from the Chinese herbal medicines Scutellaria baicalensis and Erigeron breviscapus (Vant.) Hand-Mazz, has many pharmacological effects, such as antioxidant, antitumor, antiviral, and antiinflammatory activities. Scutellarin can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts.

Scutellarin Chemical Structure

CAS No. 27740-01-8

Purity & Quality Control

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Biological Activity

Description Scutellarin (Breviscapine, Breviscapin, Scutellarein-7-glucuronide), the major active principal flavonoids extracted from the Chinese herbal medicines Scutellaria baicalensis and Erigeron breviscapus (Vant.) Hand-Mazz, has many pharmacological effects, such as antioxidant, antitumor, antiviral, and antiinflammatory activities. Scutellarin can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts.
Targets
STAT3 [6]
()
Akt [6]
()
NF-κB [7]
()
In vitro

Scutellarin protects PC12 cells against H2O2-induced cytotoxicity. It attenuates H2O2-induced intracellular accumulation of ROS and lipid peroxidation[3]. Scutellarin shows negligible inhibitory effects on the six major CYP isoenzymes in human/rat liver microsomes with almost all of the IC50 values exceeding 100 μM, whereas it shows values of 63.8 μM for CYP2C19 in human liver microsomes, and 63.1 and 85.6 μM for CYP2C7 and CYP2C79 in rat liver microsomes, respectively. Scutellarin also has weak inhibitory effect on P-gp[4]. Scutellarin strongly induces MMP-2 activation and mRNA expression in cultured HUVECs in a concentration-dependent manner. It promotes angiogenesis and may form a basis for angiogenic therapy[5].

In vivo

The pharmacokinetic results indicate that scutellarin undergoes rapid and extensive biotransformation in vivo. After intraperitoneal injection. scutellarin is absorbed rapidly. The relative bioavailability of oral administration is very low (10.67 %±4.78 %)[1]. Scutellarin has a series of protective effects including dilate blood vessels, improving microcirculation, decreasing the viscosity of blood, reducing the blood platelet count, inhibiting platelet aggregation activity etc[2].

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: PC12 cells
  • Concentrations: 1 and 10 μmol/L
  • Incubation Time: 30 min
  • Method:

    Experiments are carried out 24-48 h after the cells are seeded into plates or dishes. To produce an oxidative stress, H2O2 is freshly prepared from a 30% stock solution prior to each experiment. Preincubation of cells with scutellarin is conducted for 30 min before H2O2 is added. Assays for cell viability, ROS, lipid peroxide, and apoptosis are performed at 6 or 12 h after addition of H2O2.

  • (Only for Reference)
Animal Research:

[2]

  • Animal Models: Male Wistar rats
  • Dosages: 0.09, 0.17, 0.35, 0.70, 1.40 mmol/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 50 mg/mL
(108.14 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 462.36
Formula

C21H18O12

CAS No. 27740-01-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=CC(=O)C3=C(C(=C(C=C3O2)OC4C(C(C(C(O4)C(=O)O)O)O)O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02559960 Suspended Drug: Breviscapine Powder-Injection Adverse Drug Event|Adverse Drug Reaction|Chinese Medicine|Anaphylactic Reaction Zhong Wang|China Academy of Chinese Medical Sciences September 2015 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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