research use only

Sulforaphane Nrf2 activator

Name: Sulforaphane
Brand: Selleck Chemicals
SKU: S5771
Availability: InStock
Rating: 5 (23 reviews)

Cat.No.S5771

Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. This compound is also an inhibitor of histone deacetylase (HDAC) and NF-κB. It increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). It induces cell cycle arrest and apoptosis.

Chemical Structure

Molecular Weight: 177.29

Jump to

Quality Control

Batch: Purity: 99.97%

99.97

Related Targets	PD-1/PD-L1 CXCR STING AhR Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX
Other Nrf2 Inhibitors	Bardoxolone Methyl (RTA 402) ML385 Brusatol TBHQ Oltipraz Bardoxolone (CDDO) 4-Octyl Itaconate (4-OI) KI696 CDDO-Im (RTA-403) Mangiferin

Solubility

In vitro
Batch:

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (28.20mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.250mg/ml (7.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	177.29	Formula	C₆H₁₁NOS₂	Storage (From the date of receipt)	2 years -20°C(in the dark) liquid
CAS No.	4478-93-7	--		Storage (From the date of receipt)	2 years -20°C(in the dark) liquid

Read more about storage stability CAS number

Mechanism of Action

Targets/IC₅₀/Ki	Nrf2 HDAC NF-κB ROS HO-1
In vitro	Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that this compound induces autophagy by activating ROS/p53.
In vivo	Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.
References	[1] https://pubmed.ncbi.nlm.nih.gov/30355942/ [2] https://pubmed.ncbi.nlm.nih.gov/25936432/ [3] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643355/ [4] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947770/

Applications

Methods	Biomarkers	PMID
Western blot	EGFR / p-EGFR / STAT3 / p-STAT3 / AKT / p-AKT β-catenin / Cyclin D1	26036303
Growth inhibition assay	Cell viability	30226534
Immunofluorescence	Gli1 / Gli2 HER2	23029396

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-06-27)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07040280	NOT_YET_RECRUITING	Melanoma	Eastern Cooperative Oncology Group	2025-10-31	PHASE2
NCT07652866	NOT_YET_RECRUITING	Schizophrenia Disorder	Second Xiangya Hospital of Central South University	2026-06-02
NCT03934905	RECRUITING	Anthracycline Related Cardiotoxicity in Breast Cancer	Texas Tech University Health Sciences Center	2022-06-01	PHASE1; PHASE2
NCT07668596	RECRUITING	Immunometabolism; Mitochondrial Function, Bioenergetics; Inflammation	University of British Columbia	2026-03-01
NCT07297576	RECRUITING	ALL (Acute B-Lymphoblastic Leukemia); AML (Acute Myelogenous Leukemia; MDS (Myelodysplastic Syndrome)	Institute of Hematology & Blood Diseases Hospital, China	2025-10-22
NCT03932136	ACTIVE_NOT_RECRUITING	Clinical High Risk Syndrome of Psychosis	Shanghai Jiao Tong University School of Medicine	2019-07-14	PHASE3

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):