Sulforaphane

Catalog No.S5771

For research use only.

Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.

Sulforaphane Chemical Structure

CAS No. 4478-93-7

Selleck's Sulforaphane has been cited by 6 Publications

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Biological Activity

Description Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.
Targets
Nrf2 [1] HDAC [1] NF-κB [1] ROS [1] HO-1 [1]
In vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that CC90003 induces autophagy by activating ROS/p53.[3]

Assay
Methods Test Index PMID
Western blot β-catenin / Cyclin D1 ; EGFR / p-EGFR / STAT3 / p-STAT3 / AKT / p-AKT 20388854 26036303
Growth inhibition assay Cell viability 30226534
Immunofluorescence HER2 ; Gli1 / Gli2 30226534 23029396
In vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.[4]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: Human CRC cell HCT-116 and SW620
  • Concentrations: 20 μM
  • Incubation Time: 24 h
  • Method:

    Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.

Animal Research:

[4]

  • Animal Models: diet-induced obese mice
  • Dosages: 25 μl
  • Administration: i.p.

Chemical Information

Molecular Weight 177.29
Formula

C6H11NOS2

Density 1.17 g/mL
CAS No. 4478-93-7
Storage 2 years -20°C(in the dark) liquid
Smiles CS(=O)CCCCN=C=S

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03934905 Recruiting Drug: sulforaphane|Drug: Placebo Oral Tablet Anthracycline Related Cardiotoxicity in Breast Cancer Texas Tech University Health Sciences Center June 1 2022 Phase 1|Phase 2
NCT05073523 Recruiting Other: Diet Healthy Chalmers University of Technology September 27 2021 Not Applicable
NCT05367024 Enrolling by invitation Other: Broccoli soup|Other: Courgette soup Pre-diabetes Quadram Institute Bioscience|Norfolk and Norwich University Hospitals NHS Foundation Trust September 27 2021 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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