Sulforaphane

For research use only.

Catalog No.S5771

3 publications

Sulforaphane Chemical Structure

CAS No. 4478-93-7

Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.

Selleck's Sulforaphane has been cited by 3 publications

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Biological Activity

Description Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.
Targets
Nrf2 [1]
()
HDAC [1]
()
NF-κB [1]
()
ROS [1]
()
HO-1 [1]
()
Assay
Methods Test Index PMID
Western blot
β-catenin / Cyclin D1 ; 

PubMed: 20388854     


Sulforaphane decreased protein levels of β-catenin and cyclin D1 in both SUM159 and MCF7 cell lines.

EGFR / p-EGFR / STAT3 / p-STAT3 / AKT / p-AKT ; 

PubMed: 26036303     


Effects of sulforaphane on EGFR-related signaling in NSCLC cells. H1975, PC9/gef (a), H3255 (b) and A549 (c) cells were treated with various concentrations of sulforaphane (SFN) for 24 h, and the levels of total (t) and phosphoylated (p) proteins in the cell lysates were determined by Western blotting. β-actin was used as the loading control. The data shown are representative of three experiments with similar results

20388854 26036303
Growth inhibition assay
Cell viability; 

PubMed: 30226534     


OVCAR cells were seeded in plates and treated with sulforaphane at the concentrations shown. After 48 and 72 h, the viable cells were collected, stained and counted under a bright field microscope. WST-1 cell proliferation assay. 

30226534
Immunofluorescence
HER2; 

PubMed: 30226534     


Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100).

Gli1 / Gli2 ; 

PubMed: 23029396     


The nuclear translocation of Gli1 and Gli2, was measured by immunocytochemistry. Pancreatic CSCs were treated with sulforaphane (0–20 µM) for 24 h. Cells were then stained with anti-Gli and Gli2 antibody (green fluorescence), and DAPI (red fluorescence). Merged images are shown, which indicate yellow-orange staining of Gli 1 and Gli2 located in the nucleus due to co-localization of green and red fluorescence. 

30226534 23029396

Protocol

Solubility (25°C)

Chemical Information

Molecular Weight 177.29
Formula

C6H11NOS2

Density 1.17 g/mL
CAS No. 4478-93-7
Storage 2 years -20°C(in the dark) liquid
Synonyms N/A
Smiles CS(=O)CCCCN=C=S

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03934905 Recruiting Drug: sulforaphane|Drug: Placebo Oral Tablet Anthracycline Related Cardiotoxicity in Breast Cancer Texas Tech University Health Sciences Center December 1 2019 Phase 1|Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID