Ginsenoside Rb1

For research use only.

Catalog No.S3924 Synonyms: Gypenoside Ⅲ

Ginsenoside Rb1 Chemical Structure

Molecular Weight(MW): 1109.29

Ginsenoside Rb1 is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. Ginsenoside Rb1, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with an IC50 of 6.3±1.0 μM. Ginsenoside also inhibits IRAK-1 activation and phosphorylation of NF-κB p65.

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Description Ginsenoside Rb1 is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. Ginsenoside Rb1, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with an IC50 of 6.3±1.0 μM. Ginsenoside also inhibits IRAK-1 activation and phosphorylation of NF-κB p65.
In vitro

Ginsenoside Rb1 promotes the expressions of Nestin, NSE, GFAP and stimulates the differentiation of NSCs[1]. GRb1 is found to not only inhibit Aβ-induced ROROS overproduction and lipid peroxidation, but also to increase the Bcl-2/Bax ratio and attenuate caspase-3 activation, thereby improving cell survival. GRb1 may therefore act as a ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury[2].

In vivo Ginsenoside Rb1 can increase the ability of spatial learning and neural regeneration. It has an anti-neuroinflammatory effect in a rat model of AD. Oral ginsenoside Rb1 can metabolize ginsenoside compound K through intestinal bacteria, and then play a pharmacological role together with esterification of fatty acids to prevent memory loss and improve spatial learning. Ginsenoside Rb1 increases synaptic density and the expression of brain-derived neurotrophic factors Bcl-2 and antioxidant enzyme in hippocampus of mice, and inhibits apoptosis and calcium overload. Ginsenoside Rb1 can stimulate the release of acetylcholine and enhance the expression of choline acetyltransferase mRNA in rat brain, which contributes to the increase of hippocampal neurogenesis. It significantly increased the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus[1].

Protocol

Cell Research:[2]
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  • Cell lines: PC12 cells
  • Concentrations: 0.01 mM, 0.1 mM, 1 mM
  • Incubation Time: 24 h
  • Method: PC12 cells are grown in RPMI-1640 containing 10% heat-inactivated horse serum and 5% fetal bovine serum at 37˚C in a 5% CO2 atmosphere. The medium is changed every other day and cells are plated at the appropriate density according to each experimental scale. To study the effects of GRb1 on Aβ25-35-induced cell injury, cells are pre-incubated with GRb1 for 24 h, and then Aβ25-35 is added to the medium for an additional 24 h. The experimental groups are: A, control; B, 25 μM Aβ25-35; C, 0.01 mM GRb1 + 25 μM Aβ25-35; D, 0.1 mM GRb1 + 25 μM Aβ25-35; E, 1 mM GRb1 + 25 μM Aβ25-35. Following pre-treatment with GRb1 and treatment with Aβ, the cells are subjected to the MTT assay and flow cytometry analysis, the measurement of ROROS and MDADA, Western blotting and immunocytochemistry.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Sprague Dawley (SD) male rats
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (90.14 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1109.29
Formula

C54H92O23

CAS No. 41753-43-9
Storage powder
in solvent
Synonyms Gypenoside Ⅲ
Smiles CC(C)=CCCC(C)(OC1OC(COC2OC(CO)C(O)C(O)C2O)C(O)C(O)C1O)C3CCC4(C)C3C(O)CC5C6(C)CCC(OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C(C)(C)C6CCC45C

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID