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Ginsenoside Rb1 ATPase inhibitor

Name: Ginsenoside Rb1
Brand: Selleck Chemicals
SKU: S3924
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S3924

Ginsenoside Rb1 (Gypenoside Ⅲ) is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. This compound, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with IC50 of 6.3±1.0 μM. It also inhibits IRAK-1 activation and phosphorylation of NF-κB p65. This chemical reduces the expressions of TLR3, TLR4 and TRAF-6, and down-regulates the levels of TNF-α, IFN-β and iNOS.

Chemical Structure

Molecular Weight: 1109.29

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.07%

99.07

Related Targets	CD markers AChR Calcium Channel Sodium Channel Potassium Channel GABA Receptor TRP Channel GluR NMDAR P2 Receptor Proton Pump P-gp CFTR SGLT Chloride Channel MCT Amino acid transporter GLUT CRM1 VDAC BCRP NKCC OCT NCX OAT VMAT Aquaporin EAAT GlyT GlyR
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Chemical Information, Storage & Stability

Molecular Weight	1109.29	Formula	C₅₄H₉₂O₂₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	41753-43-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Gypenoside Ⅲ			Smiles	CC(=CCCC(C)(C1CCC2(C1C(CC3C2(CCC4C3(CCC(C4(C)C)OC5C(C(C(C(O5)CO)O)O)OC6C(C(C(C(O6)CO)O)O)O)C)C)O)C)OC7C(C(C(C(O7)COC8C(C(C(C(O8)CO)O)O)O)O)O)O)C

Solubility

In vitro
Batch:

DMSO : 100 mg/mL ( (90.14 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.500mg/ml (3.16mM)	Taking the 1 mL working solution as an example, add 50 μL of 70 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.580mg/ml (0.52mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	IRAK-1 ^[4] NF-κB ^[4] TLR3 ^[5] TLR4 ^[5] TNF-α ^[5] IFN-β ^[5] iNOS ^[5] Na+, K+-ATPase ^[3] (Cell-free assay) 6.3 μM
In vitro	Ginsenoside Rb1 promotes the expressions of Nestin, NSE, GFAP and stimulates the differentiation of NSCs^[1]. This compound is found to not only inhibit Aβ-induced ROROS overproduction and lipid peroxidation, but also to increase the Bcl-2/Bax ratio and attenuate caspase-3 activation, thereby improving cell survival. It may therefore act as a ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury^[2].
In vivo	Ginsenoside Rb1 can increase the ability of spatial learning and neural regeneration. It has an anti-neuroinflammatory effect in a rat model of AD. Oral ginsenoside Rb1 can metabolize ginsenoside compound K through intestinal bacteria, and then play a pharmacological role together with esterification of fatty acids to prevent memory loss and improve spatial learning. This compound increases synaptic density and the expression of brain-derived neurotrophic factors Bcl-2 and antioxidant enzyme in hippocampus of mice, and inhibits apoptosis and calcium overload. It can stimulate the release of acetylcholine and enhance the expression of choline acetyltransferase mRNA in rat brain, which contributes to the increase of hippocampal neurogenesis. It significantly increased the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus^[1].
References	https://pubmed.ncbi.nlm.nih.gov/29038007/ https://pubmed.ncbi.nlm.nih.gov/21472290/ https://pubmed.ncbi.nlm.nih.gov/2169691/ https://pubmed.ncbi.nlm.nih.gov/21600888/ https://pubmed.ncbi.nlm.nih.gov/25340298/

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