Shikonin

Catalog No.S8279 Synonyms: Anchusin, Anchusa acid, Alkanna Red, C.I. 75535, Isoarnebin 4, NSC 252844

Shikonin Chemical Structure

Molecular Weight(MW): 288.30

Shikonin, a potent and specific Pyruvate kinase M2 (PKM2) inhibitor, is a major component of zicao (purple gromwell, the dried root of Lithospermum erythrorhizon), a Chinese herbal medicine with various biological activities. It is also an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay.

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Purity & Quality Control

Choose Selective Anti-infection Inhibitors

Biological Activity

Description Shikonin, a potent and specific Pyruvate kinase M2 (PKM2) inhibitor, is a major component of zicao (purple gromwell, the dried root of Lithospermum erythrorhizon), a Chinese herbal medicine with various biological activities. It is also an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay.
Targets
chemokine receptor [1] PKM2 [3]
(FBP absence)
PKM2 [3]
(FBP presence)
TMEM16A chloride channel [2]
()
0.3 μM 0.8 μM 6.5 μM
In vitro

At nanomolar concentrations, shikonin inhibits monocyte chemotaxis and calcium flux in response to a variety of CC chemokines (CCL2 [monocyte chemoattractant protein 1], CCL3 [macrophage inflammatory protein 1α], and CCL5 [regulated upon activation, normal T-cell expressed and secreted protein]), the CXC chemokine (CXCL12 [stromal cell-derived factor 1α]), and classic chemoattractants (formylmethionyl-leucine-phenylalanine and complement fraction C5a). Shikonin down-regulates surface expression of CCR5, a primary HIV-1 coreceptor, on macrophages. The anti-HIV and anti-inflammatory activities of shikonin may be related to its interference with chemokine receptor expression and function[1]. Shikonin inhibits enterocyte calcium-activated chloride channels, the inhibitory effect is partially through inhibition of basolateral K+ channel activity[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A101D cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYjJcohq[mm2aX;uJI9nKGi3bXHuJGEyODGGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD63NVIhdk1? M2\NZXNCVkeHUh?=
TE-441-T cell NEnHWFhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUfJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTR2MT3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4zPCCwTR?= MY\TRW5ITVJ?
NALM-6 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{\WVGlvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk45KG6P MUnTRW5ITVJ?
SW1710 cell M1jSOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{TFW2lvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk46OSCwTR?= NFn2PFBUSU6JRWK=
ST486 cell NFTjVJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mlu4TY5pcWKrdHnvckBw\iCqdX3hckBUXDR6NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uNVghdk1? MV\TRW5ITVJ?
SK-UT-1 cell growth MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKGi3bXHuJHNMNVWWLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ1KG6P M1TTS3NCVkeHUh?=
A253 cell NWDCem9VT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkLuTY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz60PUBvVQ>? NWfvbVk5W0GQR1XS
KU812 cell Mnq5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1LiWGlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlMyKG6P MYrTRW5ITVJ?
A427 cell MmLES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXvJcohq[mm2aX;uJI9nKGi3bXHuJGE1OjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zNz65PUBvVQ>? NHLyb4hUSU6JRWK=
MZ1-PC cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGPkUFZKdmirYnn0bY9vKG:oIHj1cYFvKE2cMT3QR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE6NjJ6IH7N NFLmcVRUSU6JRWK=
NCI-H2009 cell M360Omdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmrsTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOC53NDDuUS=> Mo[2V2FPT0WU
CAL-51 cell NWHFVlNPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYTv[4JQUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytOVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOC56NTDuUS=> M4HpOXNCVkeHUh?=
GCIY cell M2XIUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEnsdGZKdmirYnn0bY9vKG:oIHj1cYFvKEeFSWmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOU46QCCwTR?= MV\TRW5ITVJ?
SK-PN-DW cell NYLBOFJpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M13QW2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvUF6tSHch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Pi53NTDuUS=> M1nVfXNCVkeHUh?=
MES-SA cell NW[zVYp6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1HPXGlvcGmkaYTpc44hd2ZiaIXtZY4hVUWVLWPBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFEvOzJibl2= MoSwV2FPT0WU
KS-1 cell MmjnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3;4WmlvcGmkaYTpc44hd2ZiaIXtZY4hU1NvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSxMlUhdk1? M1PnWXNCVkeHUh?=
IGROV-1 cell NUDVR5NDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21OU43OSCwTR?= NXHQRZlIW0GQR1XS
HMV-II cell NGSydZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnryTY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Ok41KG6P M3nHNHNCVkeHUh?=
SW982 cell NES1ZoVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEO4VGpKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OlAvOjNibl2= NEHYZ4NUSU6JRWK=
NCI-H2052 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWnJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkC1NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYyNjF|IH7N MWrTRW5ITVJ?
RT-112 cell M13DXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHz6XZpKdmirYnn0bY9vKG:oIHj1cYFvKFKWLUGxNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcxNjN5IH7N Mle1V2FPT0WU
AN3-CA cell NEDpe4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGFPOy2FQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUe5MlY2KG6P NIP3dI1USU6JRWK=
A498 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16MT65JI5O NXvtN45nW0GQR1XS
8-MG-BA cell NHjYUVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVPXS3hbUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QDVwNU[gcm0> NGrKXG9USU6JRWK=
HLE cell Moi0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIjMcZhKdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUi4MlMyKG6P M163XnNCVkeHUh?=
AM-38 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4nQ[GlvcGmkaYTpc44hd2ZiaIXtZY4hSU1vM{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25Ok4{OSCwTR?= M{m0cHNCVkeHUh?=
NCI-H1963 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIDxNZdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk3OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUCwNVEh|ryP M2HkRXNCVkeHUh?=
NKM-1 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIj4WFdKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yODF{IN88US=> MVvTRW5ITVJ?
MRK-nu-1 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmroTY5pcWKrdHnvckBw\iCqdX3hckBOWktvboWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTBzOUOg{txO NGqwVIxUSU6JRWK=
GR-ST cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWfJcohq[mm2aX;uJI9nKGi3bXHuJGdTNVOWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xNFIyQCEQvF2= M{GzN3NCVkeHUh?=
A431 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXTITlRJUW6qaXLpeIlwdiCxZjDoeY1idiCDNEOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yODZ7NzFOwG0> NX\sVIh7W0GQR1XS
SK-LMS-1 cell NGDkO2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFLWS25KdmirYnn0bY9vKG:oIHj1cYFvKFONLVzNV{0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zMEi0NUDPxE1? MnnIV2FPT0WU
MZ7-mel cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoDTTY5pcWKrdHnvckBw\iCqdX3hckBOYjdvbXXsJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOTN5MjFOwG0> Mn2yV2FPT0WU
KASUMI-1 cell M2TPbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVjpZ5U3UW6qaXLpeIlwdiCxZjDoeY1idiCNQWPVUWkuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUKzO|Eh|ryP M1fPfHNCVkeHUh?=
A204 cell NXPPW4d5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnPd3RpUW6qaXLpeIlwdiCxZjDoeY1idiCDMkC0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOjZ3NDFOwG0> NVjGSoxPW0GQR1XS
M059J cell NETKUZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnvXZJKdmirYnn0bY9vKG:oIHj1cYFvKE1yNUnKJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOzRzOTFOwG0> M{XkRXNCVkeHUh?=
NCI-H2342 cell MmruS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmjuTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKzOFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|NES1JO69VQ>? NI\V[mRUSU6JRWK=
KYSE-450 cell MkjXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4\3dmlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|OEG1JO69VQ>? NUjIO4MxW0GQR1XS
SR cell MojES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUD5VVJVUW6qaXLpeIlwdiCxZjDoeY1idiCVUjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVQxPzNizszN MVfTRW5ITVJ?
J82 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWjBPJkxUW6qaXLpeIlwdiCxZjDoeY1idiCMOEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE1OjV3IN88US=> M4TlVnNCVkeHUh?=
NTERA-S-cl-D1 cell M1fPXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWnJcohq[mm2aX;uJI9nKGi3bXHuJG5VTVKDLWOtZ4wuTDFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG0N|U4KM7:TR?= MoryV2FPT0WU
ACHN cell M33Db2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NI\iR2xKdmirYnn0bY9vKG:oIHj1cYFvKEGFSF6gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE1PjN6IN88US=> MXTTRW5ITVJ?
REH cell NUPDOIxRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHXPeoNKdmirYnn0bY9vKG:oIHj1cYFvKFKHSDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVQ3QTZizszN NV3FOWd4W0GQR1XS
MEL-HO cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJG1GVC2KTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVQ5QDJizszN MnSxV2FPT0WU
DEL cell NIrRO5FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF3TcYxKdmirYnn0bY9vKG:oIHj1cYFvKESHTDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVU{QDFizszN M{jIbnNCVkeHUh?=
IGR-1 cell M3rEN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlXmTY5pcWKrdHnvckBw\iCqdX3hckBKT1JvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVU{QThizszN NX\GO41UW0GQR1XS
RPMI-7951 cell NVLrdI1ST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1O5VWlvcGmkaYTpc44hd2ZiaIXtZY4hWlCPST23PVUyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNU[wPe69VQ>? M4nnbHNCVkeHUh?=
Daoy cell NVfHO3M5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mni5TY5pcWKrdHnvckBw\iCqdX3hckBF[W:7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOVg4PyEQvF2= MUXTRW5ITVJ?
CAL-85-1 cell M2fvZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmHITY5pcWKrdHnvckBw\iCqdX3hckBESUxvOEWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTZyN{Og{txO M2W2PXNCVkeHUh?=
SNU-423 cell M{nmSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEDPZmxKdmirYnn0bY9vKG:oIHj1cYFvKFOQVT20NlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF4M{C3JO69VQ>? NVzKNYJvW0GQR1XS
PANC-03-27 cell Mne3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXfKU2NYUW6qaXLpeIlwdiCxZjDoeY1idiCSQV7DMVA{NTJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlc1KM7:TR?= MVHTRW5ITVJ?
CHL-1 cell M1zvZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGHkR|BKdmirYnn0bY9vKG:oIHj1cYFvKEOKTD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjd6IN88US=> NXjL[FBlW0GQR1XS
SIG-M5 cell NV21SGtHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYjQfHAzUW6qaXLpeIlwdiCxZjDoeY1idiCVSVetUVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF4OUi2JO69VQ>? MVTTRW5ITVJ?
DU-4475 cell NXfSTXdHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHPK[lhKdmirYnn0bY9vKG:oIHj1cYFvKESXLUS0O|Uh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF5OUi0JO69VQ>? NHTOZldUSU6JRWK=
QIMR-WIL cell NFHKdXNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWfJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yQDF6OTFOwG0> M{fmeHNCVkeHUh?=
COLO-679 cell NWPMPHZ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXzJcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vNke5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yQDRyMTFOwG0> NIOyVW1USU6JRWK=
HOS cell NHKzU|hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkfnTY5pcWKrdHnvckBw\iCqdX3hckBJV1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG4OlA5KM7:TR?= NX7HcZpFW0GQR1XS
CA46 cell NE\2W5lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M12zTGlvcGmkaYTpc44hd2ZiaIXtZY4hS0F2NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVkyQDVizszN NVfRZVNnW0GQR1XS
DU-145 cell MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MULJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVk{ODlizszN NVPZdmlHW0GQR1XS
HAL-01 cell Ml:5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3m3fGlvcGmkaYTpc44hd2ZiaIXtZY4hUEGOLUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yQTV5MzFOwG0> NYq1ZoFUW0GQR1XS
SCC-9 cell NEfiem1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYLJcohq[mm2aX;uJI9nKGi3bXHuJHNESy17IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xPVY4PSEQvF2= NYnsbnlJW0GQR1XS
IST-SL2 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHrEUo5KdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJyMEG0JO69VQ>? M4n5fnNCVkeHUh?=
PA-1 cell MkLCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKwNVI5KM7:TR?= NULDbHlUW0GQR1XS
BT-474 cell NFTseGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4LxcGlvcGmkaYTpc44hd2ZiaIXtZY4hSlRvNEe0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zODh|NjFOwG0> MWrTRW5ITVJ?
EFM-19 cell NGPMSmVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1rkZWlvcGmkaYTpc44hd2ZiaIXtZY4hTU[PLUG5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOTJzIN88US=> NF22eY5USU6JRWK=
NB69 cell Mke1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFTUW5ZKdmirYnn0bY9vKG:oIHj1cYFvKE6ENkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyOjd|IN88US=> NEj6SZhUSU6JRWK=
SBC-5 cell MlnOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHe1To1KdmirYnn0bY9vKG:oIHj1cYFvKFOEQz21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOTV2NjFOwG0> M2O0THNCVkeHUh?=
KYSE-270 cell NXXxPHpZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{HsNWlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT2yO|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJzNkmyJO69VQ>? NXT0PJdSW0GQR1XS
P30-OHK cell NF;ocGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\6eGlvcGmkaYTpc44hd2ZiaIXtZY4hWDNyLV;IT{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjJyNEig{txO NIP5RphUSU6JRWK=
T47D cell NXjqOoxnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKyN|A3KM7:TR?= NWXac4R[W0GQR1XS
HSC-2 cell NELSXoJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlMyQCEQvF2= MkTxV2FPT0WU
HGC-27 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX3qVWlwUW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ{OEO0JO69VQ>? MnHyV2FPT0WU
YKG-1 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHJcohq[mm2aX;uJI9nKGi3bXHuJHlMTy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlg6OSEQvF2= NGXPO3VUSU6JRWK=
HCT116 cells MlTjR5l1d3SxeHnjbZR6KGG|c3H5 MX[yOEBp MkXvR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSUMTDQ[5AhfW6mZYKt[ZhxemW|c3nu[{BpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlI{KM7:TR?= MV[xO|k1QTh3OB?=
COR-L105 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUXJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzB|NDFOwG0> M4rUXnNCVkeHUh?=
LB647-SCLC cell MlTiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoLtTY5pcWKrdHnvckBw\iCqdX3hckBNSjZ2Nz3TR2xEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{M{K3PEDPxE1? MYHTRW5ITVJ?
G-401 cell NH24VnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXXFN2JnUW6qaXLpeIlwdiCxZjDoeY1idiCJLUSwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjN2OUKg{txO M2G0NnNCVkeHUh?=
MKN1 cell MkTES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVrhdYt[UW6qaXLpeIlwdiCxZjDoeY1idiCPS16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzV5NjFOwG0> NV\nXFlkW0GQR1XS
HepG2 cell NHrHbopEgXSxdH;4bYNqfHliYYPzZZk> M4jrb|I1KGh? M{P4[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GUkGgVIdxKG:4ZYLlfJBz\XO|aX7nJIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6yOEDPxE1? M3zoU|E4QTR7OEW4
HL60 cells MlXIR5l1d3SxeHnjbZR6KGG|c3H5 MnvIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OC52NDFOwG0> NHTjN5oyQTFzMUS2OC=>
MCF7 cells M4DrcWN6fG:2b4jpZ4l1gSCjc4PhfS=> Mni4O|IhcA>? NVLHPGduS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvPDVizszN MWOyNFQxOzZ2Nh?=
mouse L929 cells NEPXbYREgXSxdH;4bYNqfHliYYPzZZk> NIW1T4kzPCCq M4iwR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw6OjliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zMlIh|ryP NWDBSVJwOjF4MkC1N|A>
MDA-MB-231 cells MmOzSpVv[3Srb36gZZN{[Xl? M1nPS|I1KGh? M{XxcmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF03NjZizszN MVyyNVYzODV|MB?=
HCT15 cells NHG1TWRHfW6ldHnvckBie3OjeR?= NV;zZ3pKOjRiaB?= MkfaRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVUh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15LkOg{txO M1j6XlIyPjJyNUOw

... Click to View More Cell Line Experimental Data

In vivo Shikonin significantly delays intestinal motility in mice and reduces stool water content in a neonatal mice model of rotaviral diarrhea without affecting the viral infection process in vivo. Shikonin has been reported to have antioxidant, antibacterial, antiparasitic, antiviral and wound-healing activities. Shikonin inhibits allergic action as well as tracheal hyperresponsiveness in vivo. Intraperitonal injection of shikonin has been demonstrated to result in some toxicity, with an LD50 of 20 mg/kg. Shikonin absorption is fast if given by oral gavage and muscle injection, as it is barely detected in the plasma after 1 min, with a oral gavage yielding a bioavailability of about 34%[2].

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: Human monocytes
  • Concentrations: 10, 1, 0.1, 0.01 μM
  • Incubation Time: 12 and 24 hours
  • Method:

    Freshly isolated human monocytes are plated in a 96-microwell plate (Costar) at 105 cells per well with shikonin at a final concentration of 10, 1, 0.1, 0.01 μM. The numbers of viable cells, as detected by MTS test and the trypan blue exclusion assay, were measured 12 and 24 h after incubation at 37°C in humidified air with 5% CO2.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Neonatal ICR mice
  • Formulation: PBS
  • Dosages: 0.4 and 1.7 μg in 30 μL PBS
  • Administration: oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (197.71 mM)
Ethanol 23 mg/mL (79.77 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 288.30
Formula

C16H16O5

CAS No. 517-89-5
Storage powder
in solvent
Synonyms Anchusin, Anchusa acid, Alkanna Red, C.I. 75535, Isoarnebin 4, NSC 252844

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID