Shikonin

Catalog No.S8279 Synonyms: Anchusin, Anchusa acid, Alkanna Red, C.I. 75535, Isoarnebin 4, NSC 252844

Shikonin Chemical Structure

Molecular Weight(MW): 288.30

Shikonin, a potent and specific Pyruvate kinase M2 (PKM2) inhibitor, is a major component of zicao (purple gromwell, the dried root of Lithospermum erythrorhizon), a Chinese herbal medicine with various biological activities. It is also an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay.

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Purity & Quality Control

Choose Selective Anti-infection Inhibitors

Biological Activity

Description Shikonin, a potent and specific Pyruvate kinase M2 (PKM2) inhibitor, is a major component of zicao (purple gromwell, the dried root of Lithospermum erythrorhizon), a Chinese herbal medicine with various biological activities. It is also an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay.
Targets
chemokine receptor [1] PKM2 [3]
(FBP absence)
PKM2 [3]
(FBP presence)
TMEM16A chloride channel [2]
()
0.3 μM 0.8 μM 6.5 μM
In vitro

At nanomolar concentrations, shikonin inhibits monocyte chemotaxis and calcium flux in response to a variety of CC chemokines (CCL2 [monocyte chemoattractant protein 1], CCL3 [macrophage inflammatory protein 1α], and CCL5 [regulated upon activation, normal T-cell expressed and secreted protein]), the CXC chemokine (CXCL12 [stromal cell-derived factor 1α]), and classic chemoattractants (formylmethionyl-leucine-phenylalanine and complement fraction C5a). Shikonin down-regulates surface expression of CCR5, a primary HIV-1 coreceptor, on macrophages. The anti-HIV and anti-inflammatory activities of shikonin may be related to its interference with chemokine receptor expression and function[1]. Shikonin inhibits enterocyte calcium-activated chloride channels, the inhibitory effect is partially through inhibition of basolateral K+ channel activity[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A101D cell NXXXUXZ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWjiXlVzUW6qaXLpeIlwdiCxZjDoeY1idiCDMUCxSEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzF{IH7N MnXYV2FPT0WU
TE-441-T cell NFjudWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHzaNGpKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUS0NU1VKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5{NDDuUS=> MVHTRW5ITVJ?
NALM-6 cell MnHmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3H1UmlvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk45KG6P NE\tVnZUSU6JRWK=
SW1710 cell Mn[2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M175Z2lvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk46OSCwTR?= MXvTRW5ITVJ?
ST486 cell M2\4SGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1vM[2lvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlE5KG6P MVzTRW5ITVJ?
SK-UT-1 cell growth NVTjZ4lGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlfVTY5pcWKrdHnvckBw\iCqdX3hckBUUy2XVD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{41PCCwTR?= NEH6ZlJUSU6JRWK=
A253 cell M{nmUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mnn1TY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz60PUBvVQ>? NI[yeYFUSU6JRWK=
KU812 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml\oTY5pcWKrdHnvckBw\iCqdX3hckBMXThzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuN|Ehdk1? MXrTRW5ITVJ?
A427 cell M3;xZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIPHSVBKdmirYnn0bY9vKG:oIHj1cYFvKEF2MkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xO{46QSCwTR?= MlfSV2FPT0WU
MZ1-PC cell M1nnWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlHBTY5pcWKrdHnvckBw\iCqdX3hckBOYjFvUFOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU4zQCCwTR?= MoHvV2FPT0WU
NCI-H2009 cell M2PR[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NI\vXXdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJyLkW0JI5O NFLVPFdUSU6JRWK=
CAL-51 cell M4i5XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3PFOGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlAvQDVibl2= NWPvXVJPW0GQR1XS
GCIY cell NVzDfoxrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoHLTY5pcWKrdHnvckBw\iCqdX3hckBIS0m\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{WuPVghdk1? MoLiV2FPT0WU
SK-PN-DW cell M1;pPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNVCQLVTXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|YvPTVibl2= NFz1d2NUSU6JRWK=
MES-SA cell NFv1eWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml:1TY5pcWKrdHnvckBw\iCqdX3hckBOTVNvU1GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20NU4{OiCwTR?= MV;TRW5ITVJ?
KS-1 cell NGLFWHVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHzPNJJKdmirYnn0bY9vKG:oIHj1cYFvKEuVLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20NU42KG6P M4rU[XNCVkeHUh?=
IGROV-1 cell NIXLNnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEHjPJZKdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02PS54MTDuUS=> NVHLRYJDW0GQR1XS
HMV-II cell NH7OboZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVXJcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUW2MlQhdk1? MUTTRW5ITVJ?
SW982 cell M1jpbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF:4c|dKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OlAvOjNibl2= Ml7CV2FPT0WU
NCI-H2052 cell M3jObWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1LnTGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFUzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PjFwMUOgcm0> NHPMcIlUSU6JRWK=
RT-112 cell NYLyfW83T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkXRTY5pcWKrdHnvckBw\iCqdX3hckBTXC1zMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23NE4{PyCwTR?= MXTTRW5ITVJ?
AN3-CA cell NFLh[pRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkPDTY5pcWKrdHnvckBw\iCqdX3hckBCVjNvQ1GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23PU43PSCwTR?= M{Hp[3NCVkeHUh?=
A498 cell Ml\GS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIH3TYRKdmirYnn0bY9vKG:oIHj1cYFvKEF2OUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24NU46KG6P MYLTRW5ITVJ?
8-MG-BA cell MoXrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUXJcohq[mm2aX;uJI9nKGi3bXHuJFguVUdvQlGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24OU42PiCwTR?= MlHIV2FPT0WU
HLE cell M1zTU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWDJcohq[mm2aX;uJI9nKGi3bXHuJGhNTSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTh6LkOxJI5O MWfTRW5ITVJ?
AM-38 cell NXv0flUyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml7RTY5pcWKrdHnvckBw\iCqdX3hckBCVS1|ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUm2MlMyKG6P M2jYTnNCVkeHUh?=
NCI-H1963 cell NGjVUG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVzJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUm2N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTByMUGg{txO MmflV2FPT0WU
NKM-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2CyXmlvcGmkaYTpc44hd2ZiaIXtZY4hVkuPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlExOTJizszN MU\TRW5ITVJ?
MRK-nu-1 cell MoPiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHJcohq[mm2aX;uJI9nKGi3bXHuJG1TUy2wdT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yODF7MzFOwG0> NFHpdFZUSU6JRWK=
GR-ST cell M2D0OWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvx[Y5rUW6qaXLpeIlwdiCxZjDoeY1idiCJUj3TWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTB{MUig{txO NIHjco9USU6JRWK=
A431 cell NFHsRZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1jRcWlvcGmkaYTpc44hd2ZiaIXtZY4hSTR|MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVA3QTdizszN MkTHV2FPT0WU
SK-LMS-1 cell MlWxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUX6W4JvUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUC4OFEh|ryP MnLqV2FPT0WU
MZ7-mel cell NH;udJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzvTY5pcWKrdHnvckBw\iCqdX3hckBOYjdvbXXsJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOTN5MjFOwG0> MXvTRW5ITVJ?
KASUMI-1 cell M{H5b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFm2NGVKdmirYnn0bY9vKG:oIHj1cYFvKEuDU2XNTU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zMkO3NUDPxE1? M4PKdXNCVkeHUh?=
A204 cell NG\pVGlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYrBeIJjUW6qaXLpeIlwdiCxZjDoeY1idiCDMkC0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOjZ3NDFOwG0> MoqyV2FPT0WU
M059J cell M1LaU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3HPSGlvcGmkaYTpc44hd2ZiaIXtZY4hVTB3OVqgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE{PDF7IN88US=> NYPQ[4dOW0GQR1XS
NCI-H2342 cell NVXFN5JzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlfYTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKzOFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|NES1JO69VQ>? NXPMS5RQW0GQR1XS
KYSE-450 cell M3LlXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEO2ToVKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtOFUxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{ixOUDPxE1? NILjT2tUSU6JRWK=
SR cell NHTFdHBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEjnXXhKdmirYnn0bY9vKG:oIHj1cYFvKFOUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOFA4OyEQvF2= MVXTRW5ITVJ?
J82 cell MorLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYrIWHVrUW6qaXLpeIlwdiCxZjDoeY1idiCMOEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE1OjV3IN88US=> NGOzbnFUSU6JRWK=
NTERA-S-cl-D1 cell M2jUOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmXsTY5pcWKrdHnvckBw\iCqdX3hckBPXEWUQT3TMYNtNURzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOFM2PyEQvF2= MWLTRW5ITVJ?
ACHN cell MmXyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHvQNZdKdmirYnn0bY9vKG:oIHj1cYFvKEGFSF6gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE1PjN6IN88US=> NIfjeZRUSU6JRWK=
REH cell Mn7yS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1\a[mlvcGmkaYTpc44hd2ZiaIXtZY4hWkWKIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOFY6PiEQvF2= MVHTRW5ITVJ?
MEL-HO cell NU\vWJZJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3P5WmlvcGmkaYTpc44hd2ZiaIXtZY4hVUWOLVjPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPDh6MjFOwG0> M{LTXnNCVkeHUh?=
DEL cell NGDnUWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGRGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUWzPFEh|ryP M{jPcXNCVkeHUh?=
IGR-1 cell NFvPXHZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPyTY5pcWKrdHnvckBw\iCqdX3hckBKT1JvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVU{QThizszN NHW4XIJUSU6JRWK=
RPMI-7951 cell NY\zRXJZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3fLfWlvcGmkaYTpc44hd2ZiaIXtZY4hWlCPST23PVUyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNU[wPe69VQ>? MmS1V2FPT0WU
Daoy cell M4nEbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2HNTWlvcGmkaYTpc44hd2ZiaIXtZY4hTGGxeTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVU5PzdizszN M3HTSnNCVkeHUh?=
CAL-85-1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mnz0TY5pcWKrdHnvckBw\iCqdX3hckBESUxvOEWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTZyN{Og{txO NYH5[JNoW0GQR1XS
SNU-423 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF7Cb|BKdmirYnn0bY9vKG:oIHj1cYFvKFOQVT20NlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF4M{C3JO69VQ>? MUnTRW5ITVJ?
PANC-03-27 cell NIXsU4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUX3VVlQUW6qaXLpeIlwdiCxZjDoeY1idiCSQV7DMVA{NTJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlc1KM7:TR?= MVXTRW5ITVJ?
CHL-1 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NULjSYpzUW6qaXLpeIlwdiCxZjDoeY1idiCFSFytNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTZ5ODFOwG0> MXvTRW5ITVJ?
SIG-M5 cell MlHHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFflSodKdmirYnn0bY9vKG:oIHj1cYFvKFOLRz3NOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTZ7OE[g{txO MnzwV2FPT0WU
DU-4475 cell NEC4XG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmLPTY5pcWKrdHnvckBw\iCqdX3hckBFXS12NEe1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzl6NDFOwG0> MYfTRW5ITVJ?
QIMR-WIL cell M{P0VWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVzJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yQDF6OTFOwG0> MmLmV2FPT0WU
COLO-679 cell M3\zXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;uNHJKdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tOlc6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zOESwNUDPxE1? M{L6VnNCVkeHUh?=
HOS cell M4TVXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYjJcohq[mm2aX;uJI9nKGi3bXHuJGhQWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUi2NFgh|ryP MXrTRW5ITVJ?
CA46 cell M4T0WGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGO0UHRKdmirYnn0bY9vKG:oIHj1cYFvKEODNE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE6OTh3IN88US=> NXTIdYhmW0GQR1XS
DU-145 cell NXT4VnFXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3H6bmlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yQTNyOTFOwG0> MorKV2FPT0WU
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NB69 cell M1jOdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXLJcohq[mm2aX;uJI9nKGi3bXHuJG5DPjliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKxNlc{KM7:TR?= NIjleFBUSU6JRWK=
SBC-5 cell M1zHT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4TkfWlvcGmkaYTpc44hd2ZiaIXtZY4hW0KFLUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyPTR4IN88US=> MnXwV2FPT0WU
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P30-OHK cell NVj3PHlmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3XCfGlvcGmkaYTpc44hd2ZiaIXtZY4hWDNyLV;IT{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjJyNEig{txO MV7TRW5ITVJ?
T47D cell MlTuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUDJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKyN|A3KM7:TR?= NHjiXFZUSU6JRWK=
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COR-L105 cell NWTBdotQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzB|NDFOwG0> NWXlc4E2W0GQR1XS
LB647-SCLC cell NWHucVNQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2C0TGlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ4NEetV2NNSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkOyO|gh|ryP MXHTRW5ITVJ?
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HepG2 cell MVPDfZRwfG:6aXPpeJkh[XO|YYm= Ml7mNlQhcA>? M2rURWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GUkGgVIdxKG:4ZYLlfJBz\XO|aX7nJIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6yOEDPxE1? MVixO|k1QTh3OB?=
HL60 cells M{jwcmN6fG:2b4jpZ4l1gSCjc4PhfS=> MnTvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OC52NDFOwG0> NHHkcmEyQTFzMUS2OC=>
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mouse L929 cells NW\5eXd3S3m2b4TvfIlkcXS7IHHzd4F6 NFXHS2MzPCCq NFy0XWNEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNQTJ7IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9N{4zKM7:TR?= NVSy[HVmOjF4MkC1N|A>
MDA-MB-231 cells MWPGeY5kfGmxbjDhd5NigQ>? MU[yOEBp MXXBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9Ok43KM7:TR?= MXiyNVYzODV|MB?=
HCT15 cells NFL6SlhHfW6ldHnvckBie3OjeR?= MnnJNlQhcA>? NHLGZ4lCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyPSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUeuN{DPxE1? NWDOXHFnOjF4MkC1N|A>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Bcl2 / Bax / CytC / Apaf1 / AIF / Caspase-9 / Caspase-3 / Caspase-7 ; 

PubMed: 25879420     


Shikonin treatment alters expression of mitochondrial proteins in DU-145 and PC-3 cells. Cells were treated with Shikonin for 0-24 h, expression of indicated mitochondrial proteins were performed as described in Methods. Experiments were repeated minimum two-four times.

p-PERK / PERK / p-eIF2α / eIF2α / GRP78 / CHOP ; 

PubMed: 25879420     


Shikonin treatment induces ER stress associated protein expression. Western blot experiments in prostate cancer cells treated with 2.5 μM of Shikonin increased the phosphorylation of PERK, and its substrate eIF2α, increased expression of GRP78/Bip and CHOP/GADD135 in a time-dependent manner.

MMP-2 / MMP-9 ; 

PubMed: 26473829     


Shikonin inhibited the expression and activity of MMP-2 and MMP-9. U87 and U251 cells were treated with shikonin at 2.5, 5, and 7.5 μmol/L for 48 h. Serum free DMEM served as a negative control. Expressions of MMP-2 and MMP-9 were checked with Western Blot. (A) Effects of shikonin on the expression of MMP-2 in U87 cells; (B) Effects of shikonin on the expression of MMP-9 in U87 cells; (C) The changing pattern of MMP-2 in U251 cells; (D) The changing pattern of MMP-9 in U251 cells; (E) Effects of shikonin on the activities of MMP-2 and MMP-9 in U87 cells; (F) Effects of shikonin on the activities of MMP-2 and MMP-9 in U251 cells. Statistical analysis showed that shikonin significantly reduced the expression levels and activity of both MMP-2 and MMP-9 proteins compared with the control group in a dose-dependent manner. * p < 0.05, ** p < 0.01 (compared with control group); # p < 0.05 (compared with 2.5 μmol/L groups) (n = 5).

p-β-catenin / β-catenin ; 

PubMed: 26473829     


(A) Western blot assay results of β-catenin and p-β-catenin Y333 in U87 cells. (D) Western blot assay results of β-catenin and p-β-catenin Ser45 in U87 cells.

25879420 26473829
Growth inhibition assay
Cell viability; 

PubMed: 25879420     


Shikonin treatment inhibits prostate cancer cell viability without affecting normal prostate epithelial cells. DU-145, PC-3 and PrEC cells were treated with various doses (0.5, 1, 2.5, 5 and 10 μM) of Shikonin for 24 h and CCK-8 assay was used for the measurement of cell viability.

25879420
In vivo Shikonin significantly delays intestinal motility in mice and reduces stool water content in a neonatal mice model of rotaviral diarrhea without affecting the viral infection process in vivo. Shikonin has been reported to have antioxidant, antibacterial, antiparasitic, antiviral and wound-healing activities. Shikonin inhibits allergic action as well as tracheal hyperresponsiveness in vivo. Intraperitonal injection of shikonin has been demonstrated to result in some toxicity, with an LD50 of 20 mg/kg. Shikonin absorption is fast if given by oral gavage and muscle injection, as it is barely detected in the plasma after 1 min, with a oral gavage yielding a bioavailability of about 34%[2].

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: Human monocytes
  • Concentrations: 10, 1, 0.1, 0.01 μM
  • Incubation Time: 12 and 24 hours
  • Method:

    Freshly isolated human monocytes are plated in a 96-microwell plate (Costar) at 105 cells per well with shikonin at a final concentration of 10, 1, 0.1, 0.01 μM. The numbers of viable cells, as detected by MTS test and the trypan blue exclusion assay, were measured 12 and 24 h after incubation at 37°C in humidified air with 5% CO2.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Neonatal ICR mice
  • Formulation: PBS
  • Dosages: 0.4 and 1.7 μg in 30 μL PBS
  • Administration: oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (197.71 mM)
Ethanol 23 mg/mL (79.77 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 288.30
Formula

C16H16O5

CAS No. 517-89-5
Storage powder
in solvent
Synonyms Anchusin, Anchusa acid, Alkanna Red, C.I. 75535, Isoarnebin 4, NSC 252844

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID