Indole-3-carbinol

For research use only.

Catalog No.S2313

1 publication

Indole-3-carbinol Chemical Structure

CAS No. 700-06-1

Indole-3-carbinol is produced by the breakdown of the glucosinolate glucobrassicin, which can be found at relatively high levels in cruciferous vegetables. Indole-3-carbinol suppresses NF-κB and IκBα kinase activation.

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10mM (1mL in DMSO) USD 88 In stock
USD 99 In stock
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Selleck's Indole-3-carbinol has been cited by 1 publication

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  • CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Indole-3-carbinol purchased from Selleck.

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Biological Activity

Description Indole-3-carbinol is produced by the breakdown of the glucosinolate glucobrassicin, which can be found at relatively high levels in cruciferous vegetables. Indole-3-carbinol suppresses NF-κB and IκBα kinase activation.
Targets
NF-κB [1]
()
Assay
Methods Test Index PMID
Western blot
Cleaved caspase-3 / PARP ; 

PubMed: 19706645     


Dose-dependent effect of indole-3-carbinol on caspase-3 activation and PARP cleavage in MDA-MB-231 cells after 48 h exposure in 5% FBS-supplemented Dulbecco’s modified Eagle’s medium/F-12 medium. 

p-AKT / AKT / p-GSK3β / Cyclin D1 / p-IKKα / IKKα / IκB / p21 / p27 / RelA / Survivin / Bcl-2 / Bcl-xL / Mcl-1 / Bax ; 

PubMed: 19706645     


Dose-dependent effects of OSU-A9 versus indole-3-carbinol on the phosphorylation of Akt, GSK3β and IKKα and the expression levels of cyclin D1, IκB, p21, p27, RelA, survivin, Bcl-2, Bcl-xL, Mcl-1 and Bax in MCF-7 cells after 48 h exposure in 5% FBS-containing Dulbecco’s modified Eagle’s medium/F-12 medium.

p-JNK / JNK / p-p38 / p38 / GADD153 / BRCA1 / BRCA2 / AhR / Nrf2 / ERα / ERβ ; 

PubMed: 19706645     


Activation of stress response signaling by OSU-A9 and indole-3-carbinol. MCF-7 cells were exposed to the indicated concentrations of OSU-A9 and indole-3-carbinol in 5% FBS-supplemented Dulbecco’s modified Eagle’s medium/F-12 medium for 48 h. Cell lysates were immunoblotted with antibodies against various biomarkers of oxidative stress or endoplasmic reticulum stress, including p-JNK, p-p38, GADD153, BRCA1, BRCA2, AhR, Nrf2, ERα and ERβ.

MDM2; 

PubMed: 25209720     


Cell extracts were biochemically separated into nuclear enriched and cytoplasmic fractions, electrophoretically fractionated, and Western blots probed with antibodies specific for MDM2, the cytoplasmic marker HSP90 and the nuclear marker lamin. 

19706645 25209720
Growth inhibition assay
Cell viability; 

PubMed: 25447674     


(B, C) I3C reduces HCC cell proliferation in culture. SK-Hep-1 cells and SNU-449 cells were seeded in 96-well plates, respectively, and incubated overnight to allow attachment before starting the treatments with DMSO alone or different doses of indole-3-carbinol (I3C) dissolved in DMSO. Cell viability was assessed at indicated time points by MTT.

25447674
Immunofluorescence
MDM2; 

PubMed: 25209720     


The subcellular localization of MDM2 and nucleostemin was determined by indirect immunofluorescence microscopy. DAPI staining was used to visualize DNA stained nuclei. Scale bar represents 4 μm. DAPI, 4',6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; I3C, indole-3-carbinol; MDM2, murine double mutant 2; NS, nucleostemin; siRNA, small interfering RNA.

25209720

Protocol

Solubility (25°C)

In vitro DMSO 29 mg/mL (197.03 mM)
Ethanol 29 mg/mL (197.03 mM)
Water 7 mg/mL (47.56 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 147.18
Formula

C9H9NO

CAS No. 700-06-1
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)C(=CN2)CO

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID