SC75741
Catalog No.S7273
Molecular Weight(MW): 565.67
SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM.
6 Customer Reviews
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Immunofluorescence of NF-κB (p65) (200×). SK-OV-3 and A2780S cells were treated with different compounds (vehicle, 5 μM JSH-23, 5 μM SC75741, 5 μM CAPE, 4 μM MIL, 4 μM MIL combined with pre-treated 5 μM JSH-23, 4 μM MIL combined with pre-treated 5 μM SC75741, 4 μM MIL combined with pre-treated 5 μM CAPE) for 48 h. NF-κB(p65) was detected by a fluorescence microscopy. The nuclei were stained with Hoechst 33258.
Oncotarget, 2016, 7(32):52281-52293. SC75741 purchased from Selleck.
Antiproliferation and antimigration effects of EGCG in normal and NF-κB-inhibited SW780 cells. (A) NF-κB was inhibited in SW780 cells after treatment with SC75741. Antiproliferation (B) and antimigration (C) effects of EGCG in normal and NF-κB-inhibited SW780 cells. Data were expressed as mean±S.D., n=3.
J Nutr Biochem, 2016, 41:56-64. . SC75741 purchased from Selleck.
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NF-κB was inhibited in SW780 cells after treatment with SC75741
J Nutr Biochem, 2017, 41:56-64. SC75741 purchased from Selleck.
HMEECs were pre-treated with SC75741 (1 μM) for 2 h, followed by LPS (100 ng/ml) treatment for another 24 h. Then, western blot analysis was used to calculate (E) Nrf-2 and Keap-1 expressions in cells. Data are expressed as the mean ± SEM(n = 8). *p < 0.05, **p < 0.01 and ***p < 0.001 versus the control (Con) group. +p < 0.05,++p < 0.01 and +++p < 0.001versus the LPS group.##p < 0.01 and ###p < 0.001.
Biomed Pharmacother, 2019, 109:1978-1987. SC75741 purchased from Selleck.
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(A) To eliminate the interference of tool drugs on apoptosis and to choose suitable drug concentrations, we detected the survival rates of cells using MTT assays. The results showed that the survival rates of the SC75741 (1 µM) and JSH-23 (2 µM) groups displayed no statistically significant differences compared to those of control group (n=3–4). (B) SC75741, an NF-κB inhibitor, reversed the anti-apoptotic functions of Cx32 in response to TNFα (n=3).
Int J Oncol, 2017, 51(4):1159-1168. SC75741 purchased from Selleck.
(A) B-CPAP/KLF5 cells were treated with SC75741 or DMSO for 48 h. Western blotting of cell lysates from B-CPAP/NC, B-CPAP/KLF5, B-CPAP/KLF5+DMSO and B-CPAP/KLF5+SC75741 cells revealed that the NF-κB inhibitor SC75741 decreased the nuclear NF-κB p65 level of B-CPAP/KLF5 cells; and p84 was used as a nuclear marker.
Oncol Rep, 2018, 40(5):2608-2618. SC75741 purchased from Selleck.
Purity & Quality Control
Choose Selective NF-κB Inhibitors
Biological Activity
| Description | SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. | |||||||||||||||||||||||
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| Features | NF-κB-selective inhibitor. | |||||||||||||||||||||||
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| In vitro |
SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. [1] SC75741 inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, SC75741 shows a high barrier for development of resistant virus variants. [2] |
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| Cell Data |
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| Assay |
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| In vivo | SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. [2] |
Protocol
| Kinase Assay:[1] |
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NF-κB reporter gene assay: The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay. |
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| Cell Research:[1] |
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| Animal Research:[2] |
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Solubility (25°C)
| In vitro | DMSO | 35 mg/mL warmed (61.87 mM) |
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| Water | Insoluble | |
| Ethanol | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 565.67 |
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| Formula | C29H23N7O2S2 |
| CAS No. | 913822-46-5 |
| Storage | powder |
| in solvent | |
| Synonyms | N/A |
Bio Calculators
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Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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