For research use only.

Catalog No.S7273

29 publications

SC75741 Chemical Structure

CAS No. 913822-46-5

SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.

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Selleck's SC75741 has been cited by 29 publications

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Choose Selective NF-κB Inhibitors

Biological Activity

Description SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.
Features NF-κB-selective inhibitor.
NF-κB [1]
(Cell-based NF-κB reporter gene assay)
200 nM(EC50)
In vitro

SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. [1] SC75741 inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, SC75741 shows a high barrier for development of resistant virus variants. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 cells M4XzWmZ2dmO2aX;uJIF{e2G7 Mnq0OUBp NWj1VVVTUW6qaXLpeIlwdiCxZjDOSk1s[XCyYVKgbY4hSTV2OTDj[YxteyCjZoTldkA2KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvOiEQvF2= NILxOIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i2PVUyOid-MUe4Olk2OTJ:L3G+
PBMC MknHVJJwdGmoZYLheIlwdiCjc4PhfS=> NGTUc2VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDCUWMtKEmFNUC9Nk4zKM7:TR?= NXq2XIJwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4Olk2OTJpPkG3PFY6PTF{PD;hQi=>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
TRIM44 / Vimentin / E-cadherin / Slug / p-p65 / p-AKT; 

PubMed: 30922374     

Western blot was used to detect levels of TRIM44, EMT markers, p-P65, P65, p-AKT, and AKT in A375-Vector and A375-TRIM44 cells after incubation with LY-294002, SC75741, or both.

NF-κB(p65) ; 

PubMed: 27447570     

Immunofluorescence of NF-κB (p65) (200×). SK-OV-3 and A2780S cells were treated with different compounds (vehicle, 5 μM JSH-23, 5 μM SC75741, 5 μM CAPE, 4 μM MIL, 4 μM MIL combined with pre-treated 5 μM JSH-23, 4 μM MIL combined with pre-treated 5 μM SC75741, 4 μM MIL combined with pre-treated 5 μM CAPE) for 48 h. NF-κB(p65) was detected by a fluorescence microscopy. The nuclei were stained with Hoechst 33258.

In vivo SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. [2]


Kinase Assay:[1]
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NF-κB reporter gene assay:

The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.
Cell Research:[1]
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  • Cell lines: Human PBMCs
  • Concentrations: ~50 μM
  • Incubation Time: 48 hours
  • Method: Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: C57BL/6 mice infected with H5N1 influenza virus
  • Dosages: ~15 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL warmed (61.87 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 565.67


CAS No. 913822-46-5
Storage powder
in solvent
Synonyms N/A
Smiles C1CN(CCC1C2=NC(=CS2)C(=O)NC3=NC4=C(N3)C=C(C=C4)C(=O)C5=CC=CC=C5)C6=NC=NC7=C6SC=C7

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID