Catalog No.S7273

For research use only.

SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.

SC75741 Chemical Structure

CAS No. 913822-46-5

Selleck's SC75741 has been cited by 37 publications

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Other NF-κB Products

Biological Activity

Description SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.
Features NF-κB-selective inhibitor.
NF-κB [1]
(Cell-based NF-κB reporter gene assay)
200 nM(EC50)
In vitro

SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. [1] SC75741 inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, SC75741 shows a high barrier for development of resistant virus variants. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 cells MV3GeY5kfGmxbjDhd5NigQ>? MX[1JIg> NVLDcJpOUW6qaXLpeIlwdiCxZjDOSk1s[XCyYVKgbY4hSTV2OTDj[YxteyCjZoTldkA2KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvOiEQvF2= M37YclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OE[5OVEzLz5zN{i2PVUyOjxxYU6=
PBMC NVvudFZWWHKxbHnm[ZJifGmxbjDhd5NigQ>? M1rEc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFLNR{whUUN3ME2yMlIh|ryP MoPKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6Nkm1NVIoRjF5OE[5OVEzRC:jPh?=
Methods Test Index PMID
Western blot TRIM44 / Vimentin / E-cadherin / Slug / p-p65 / p-AKT 30922374
Immunofluorescence NF-κB(p65) 27447570
In vivo SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. [2]

Protocol (from reference)

Kinase Assay:[1]
  • NF-κB reporter gene assay:

    The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.

Cell Research:[1]
  • Cell lines: Human PBMCs
  • Concentrations: ~50 μM
  • Incubation Time: 48 hours
  • Method: Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.
Animal Research:[2]
  • Animal Models: C57BL/6 mice infected with H5N1 influenza virus
  • Dosages: ~15 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 35 mg/mL warmed
(61.87 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 565.67


CAS No. 913822-46-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CN(CCC1C2=NC(=CS2)C(=O)NC3=NC4=C(N3)C=C(C=C4)C(=O)C5=CC=CC=C5)C6=NC=NC7=C6SC=C7

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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