SC75741

Catalog No.S7273

SC75741 Chemical Structure

Molecular Weight(MW): 565.67

SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM.

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Cited by 7 Publications

6 Customer Reviews

  • Immunofluorescence of NF-κB (p65) (200×). SK-OV-3 and A2780S cells were treated with different compounds (vehicle, 5 μM JSH-23, 5 μM SC75741, 5 μM CAPE, 4 μM MIL, 4 μM MIL combined with pre-treated 5 μM JSH-23, 4 μM MIL combined with pre-treated 5 μM SC75741, 4 μM MIL combined with pre-treated 5 μM CAPE) for 48 h. NF-κB(p65) was detected by a fluorescence microscopy. The nuclei were stained with Hoechst 33258.

    Oncotarget, 2016, 7(32):52281-52293. SC75741 purchased from Selleck.

    Antiproliferation and antimigration effects of EGCG in normal and NF-κB-inhibited SW780 cells. (A) NF-κB was inhibited in SW780 cells after treatment with SC75741. Antiproliferation (B) and antimigration (C) effects of EGCG in normal and NF-κB-inhibited SW780 cells. Data were expressed as mean±S.D., n=3.

    J Nutr Biochem, 2016, 41:56-64. . SC75741 purchased from Selleck.

  • NF-κB was inhibited in SW780 cells after treatment with SC75741

    J Nutr Biochem, 2017, 41:56-64. SC75741 purchased from Selleck.

    HMEECs were pre-treated with SC75741 (1 μM) for 2 h, followed by LPS (100 ng/ml) treatment for another 24 h. Then, western blot analysis was used to calculate (E) Nrf-2 and Keap-1 expressions in cells. Data are expressed as the mean ± SEM(n = 8). *p < 0.05, **p < 0.01 and ***p < 0.001 versus the control (Con) group. +p < 0.05,++p < 0.01 and +++p < 0.001versus the LPS group.##p < 0.01 and ###p < 0.001.

    Biomed Pharmacother, 2019, 109:1978-1987. SC75741 purchased from Selleck.

  • (A) To eliminate the interference of tool drugs on apoptosis and to choose suitable drug concentrations, we detected the survival rates of cells using MTT assays. The results showed that the survival rates of the SC75741 (1 µM) and JSH-23 (2 µM) groups displayed no statistically significant differences compared to those of control group (n=3–4). (B) SC75741, an NF-κB inhibitor, reversed the anti-apoptotic functions of Cx32 in response to TNFα (n=3).

    Int J Oncol, 2017, 51(4):1159-1168. SC75741 purchased from Selleck.

    (A) B-CPAP/KLF5 cells were treated with SC75741 or DMSO for 48 h. Western blotting of cell lysates from B-CPAP/NC, B-CPAP/KLF5, B-CPAP/KLF5+DMSO and B-CPAP/KLF5+SC75741 cells revealed that the NF-κB inhibitor SC75741 decreased the nuclear NF-κB p65 level of B-CPAP/KLF5 cells; and p84 was used as a nuclear marker.

    Oncol Rep, 2018, 40(5):2608-2618. SC75741 purchased from Selleck.

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM.
Features NF-κB-selective inhibitor.
Targets
NF-κB [1]
(Cell-based NF-κB reporter gene assay)
200 nM(EC50)
In vitro

SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. [1] SC75741 inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, SC75741 shows a high barrier for development of resistant virus variants. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 cells M3zEcGZ2dmO2aX;uJIF{e2G7 NGXkVWI2KGh? NWjZRWtKUW6qaXLpeIlwdiCxZjDOSk1s[XCyYVKgbY4hSTV2OTDj[YxteyCjZoTldkA2KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvOiEQvF2= NYjLOI5VOTd6Nkm1NVI>
PBMC Mn7CVJJwdGmoZYLheIlwdiCjc4PhfS=> NXPxenpRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRm1ENCCLQ{WwQVIvOiEQvF2= MoPFNVc5Pjl3MUK=

... Click to View More Cell Line Experimental Data

In vivo SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. [2]

Protocol

Kinase Assay:[1]
+ Expand

NF-κB reporter gene assay:

The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.
Cell Research:[1]
+ Expand
  • Cell lines: Human PBMCs
  • Concentrations: ~50 μM
  • Incubation Time: 48 hours
  • Method: Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: C57BL/6 mice infected with H5N1 influenza virus
  • Formulation: 10% DMSO, 30% Cremophor EL and 60% PBS
  • Dosages: ~15 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL warmed (61.87 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 565.67
Formula

C29H23N7O2S2

CAS No. 913822-46-5
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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NF-κB Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID