Astragaloside IV

Catalog No.S3901 Synonyms: AST-IV, AS-IV

For research use only.

Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.

Astragaloside IV Chemical Structure

CAS No. 84687-43-4

Selleck's Astragaloside IV has been cited by 1 Publication

Purity & Quality Control

Choose Selective ERK Inhibitors

Biological Activity

Description Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.
Targets
JNK [2] ERK [2] Akt [2] mTOR [2] NF-κB [2]
In vitro

Astragaloside IV probably alleviates leptin resistance by comprehensive modulation of ObR/STAT3 signaling transduction in neuronal cells[2].

In vivo Administration of astragaloside IV (AS-IV) reduces albuminuria, ameliorates changes in the glomerular and tubular pathology, and decreases urinary NAG, NGAL, and TGF-β1 in db/db mice. AS-IV also attenuates the diabetes-related activation of Akt/mTOR, NFκB, and Erk1/2 signaling pathways without causing any detectable hepatotoxicity. AS-IV lowers blood glucose levels in streptozotocin (STZ)-induced diabetic models. It prevents body weight gain and reduced accumulation of fat tissues in HFD fed mice. Astragaloside IV actively participates in the modulation of energy homeostasis[1][2].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: SH-SY5Y cells
  • Concentrations: 0, 25, 50 and 100 μM
  • Incubation Time: 24 h
  • Method:

    Neuronal cell line SH-SY5Y cells are maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator supplied with 5% CO2. After seeded in 6-well plate at 5 × 105 cells/ml and cultured without FBS overnight, the cells are treated with ASI (0, 25, 50 and 100 μM) for 24 h. Thereafter, they are harvested with CelLyticTM MT mammalian tissue lysis reagent supplemented with protease and phosphatase inhibitors followed by sonication, and stored at −80℃ for further western blotting analysis.

Animal Research:

[2]

  • Animal Models: Six-weeks old male C57BL/6 mice
  • Dosages: 25 mg/kg/d
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 784.97
Formula

C41H68O14

CAS No. 84687-43-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1(C(CCC23C1C(CC4C2(C3)CCC5(C4(CC(C5C6(CCC(O6)C(C)(C)O)C)O)C)C)OC7C(C(C(C(O7)CO)O)O)O)OC8C(C(C(CO8)O)O)O)C

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