Curcumin

Catalog No.S1848 Synonyms: Diferuloylmethane

Curcumin Chemical Structure

Molecular Weight(MW): 368.38

Curcumin is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase(IC50~25 μM) and Histone deacetylase; activates Nrf2 pathway and supresses the activation of transcription factor NF-κB.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 8 Publications

7 Customer Reviews

  • EC9706 and TE13 cells were treated with different concentrations of curcumin for 24 h. Then, cells were prepared for immunoblotting against p-JAK2, JAK2 and GAPDH.

    J Exp Clin Cancer Res, 2018, 37(1):303. Curcumin purchased from Selleck.

    Curcumin increased the expression of FOXA2 in NCI-H292 cells. Cultured NCI-H292 cells were treated with curcumin (40 μM) or vehicle (DMSO, control) at different time intervals. The mRNA and protein expression level of FOXA2 were determined via qRT-PCR (A) and western blotting (B), respectively. Data are presented as mean ± SEM from three independent, ∗p < 0.05, ∗∗p < 0.01.

    Front Pharmacol, 2018, 9:60. Curcumin purchased from Selleck.

  • Effect of NC1, NC1-CUR, NC2 and NC2-CUR on SH-SY5Y cell viability. The cells were treated for 24 h with different dilutions(1:1, 1:2 and 1:4) of empty nanocapsules (NC1 and NC2) and nanocapsules containing curcumin (NC1-CUR and NC2-CUR) followed by measurement of cell viability by MTT reduction assay (panel A) and cell toxicity by LDH release assay (panel B). Data after normalization to vehicle-treated cells (100%, MTT assay) or to total LDH release (TritonX100-treated cells, 100%) are presented as a mean±SEM from 3-8 independent experiments with five replicates. *p < 0.05, **p < 0.01 and ***p<0.001 versus vehicle-treated cells; &&&p<0.001 NC1 versus NC1-CUR.

    Nanotechnology, 2016, 27(35):355101. Curcumin purchased from Selleck.

    Translocation of AIF from the cytosol to the nucleus after RN5 cells were treated with curcumin for 24 h. Following incubation with an AIF primary antibody, cells were stained with an Alexa Fluor-555-conjugated goat anti-rabbit immunoglobulin G secondary antibody. Nuclei were stained with DAPI. Scale bar, 25 µm. AIF, apoptosis-inducing factor; DMSO, dimethyl sulfoxide.

    Int J Oncol, 2018, 53(6):2531-2541. Curcumin purchased from Selleck.

  • Oncol Res 2014 21(3), 137-44. Curcumin purchased from Selleck.

    Oncol Res 2014 21(3), 137-44. Curcumin purchased from Selleck.

  • Inhibition of [3H]uridine uptake in MIA PaCa-2 (A & B) and PANC-1 (C) cells by curcumin (CUR) and A13. Results are normalised to the disintegrations/min (dpm) of DMSO-only control and are means ±S.D. of n =3 independent experiments done in triplicates. ***P<0.001 significantly different from DMSO-control, calculated using one-way ANOVA and Dunnett's post-hoc test. NBMPR-Nitrobenzylthioinosine. Conc-concentration.

    Eur J Pharmacol, 2017, 803:167-173. Curcumin purchased from Selleck.

Purity & Quality Control

Choose Selective Histone Acetyltransferase Inhibitors

Biological Activity

Description Curcumin is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase(IC50~25 μM) and Histone deacetylase; activates Nrf2 pathway and supresses the activation of transcription factor NF-κB.
Targets
Nrf2 [1]
(Cell-free assay)
HDAC [7]
(Cell-free assay)
NF-κB [8]
(Cell-free assay)
p300 histone acetylatransferase [6]
(Cell-free assay)
~25 μM
In vitro

Curcumin induces the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. Curcumin inhibits cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1[2]. Curcumin induces endoplasmic reticulum (ER) stress and mitochondrial dysfunction as evidenced by up-regulation of CCAAT/enhancer binding protein homologous protein (CHOP), phosphorylation of JNK and down-regulation of SERCA2ATPase, release of cytochrome c, decrease of Bcl-2 and reduction of mitochondrial membrane potential in both AGS and HT-29 cells[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U87 NHG3U4pE\WyuII\pZYJqdGm2eTDhd5NigQ>? MV:wMEAyOCxiMkCsJFMxNCB2MDygZY5lKDVyINM1UU9N NFLzS3IzPCCqb4Xydy=> Mk\vRZQh[2:wY3XueJJifGmxboOgbIlocGW{IIToZY4hOjBiwsXNM2wtKGOnbHygeoli[mmuaYTp[ZMhe3SnZYDsfUBl\WOuaX7l[C=> NWO1SG0{OzFyNkK1Nlc>
HEL cells MVvD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NXr3PHFSOC1|MDFCuY1wdC:O MUGyOE81QMLiaH;1dpM> Mnq3WIhmKH[rYXLpcIl1gSCxZjD0bIUhUEWOIHPlcIx{KGW6cH;z[YQhfG9iY4XyZ5VucW5id3HzJJNq\26rZnnjZY51dHlibH;3[ZIh[2:vcHHy[YQhfG9iY3;ueJJwdCClZXzsd{BmfmWwIHH0JIxwfyClb37j[Y51emG2aX;ud{4> M{ToOVMyODN|MkC5
Raw246.7 M4jlR2NmdGxidnnhZoltcXS7IHHzd4F6 MnzUNUwhPSxiMUCsJFUxNCBzMECsJFIxOCxiYX7kJFUxOCCpL33M NXXoOpp6OzFyMEe3NFE>
RT112 NHLCUWdE\WyuII\pZYJqdGm2eTDhd5NigQ>? NHPsSG8xNjFib4KgNE41KM7:Zz;tcC=> NGHmZY0zPCxiNEisJIFv\CB5MjDo MYDDeZJkfW2rbjDhcI9v\SxiYXTk[YQh[XRiYTDjc45k\W62cnH0bY9vKG[{b32gNE4yKHSxIECuOEDPxGdxbXysJIRq\CCwb4SgZYx1\XJiY3XscEBoem:5dHigc4YhWlRzMUKgZ4VtdHNuIIPsbYdpfGy7IILl[JVk\WRiVV3VR|Mh[2WubDDndo94fGhiYYSgNE41KM7:Zz;tcEwh[W6mIH;ucJkhdW:mZYLheIVtgSC|dYDwdoV{e2WmIHfyc5d1cCCxZjDUR2NUXVBiYYSgNE4zKGGwZDCwMlQh|rypL33sMEBm[WOqIHPvcZBiemWmIITvJJJme3CnY4TpeoUh[2:wdILvcJM> M3;uSFMxQTh2Mke4
UMUC3 MYfD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NX;NOZBmOC5zIH;yJFAvPMLizsznM41t MUWyOEwhPDhuIHHu[EA4OyCq MYTDeZJkfW2rbjDhcI9v\SxiYXTk[YQh[XRiYTDjc45k\W62cnH0bY9vKG[{b32gNE4yKHSxIECuOEDPxGdxbXysJIRq\CCwb4SgZYx1\XJiY3XscEBoem:5dHigc4YhWlRzMUKgZ4VtdHNuIIPsbYdpfGy7IILl[JVk\WRiVV3VR|Mh[2WubDDndo94fGhiYYSgNE41KM7:Zz;tcEwh[W6mIH;ucJkhdW:mZYLheIVtgSC|dYDwdoV{e2WmIHfyc5d1cCCxZjDUR2NUXVBiYYSgNE4zKGGwZDCwMlUh|rypL33sMEBm[WOqIHPvcZBiemWmIITvJJJme3CnY4TpeoUh[2:wdILvcJM> MYWzNFk5PDJ5OB?=
TCCSUP MlixR4VtdCC4aXHibYxqfHliYYPzZZk> NG\Ue4wxNjFib4KgNE41yqEQvHevcYw> MWmyOEwhPDhuIHHu[EA4PCCq NH7od5lEfXKldX3pckBidG:wZTygZYRl\WRiYYSgZUBkd26lZX70doF1cW:wIH\yc40hOC5zIITvJFAvPCEQvHevcYwtKGSrZDDuc5Qh[Wy2ZYKgZ4VtdCCpcn;3eIghd2ZiUmSxNVIh[2WubIOsJJNtcWeqdHz5JJJm\HWlZXSgWW1WSzNiY3XscEBoem:5dHigZZQhOC52IN88[{9udCxiYX7kJI9vdHlibX;k[ZJifGWueTDzeZBxemW|c3XkJIdzd3e2aDDv[kBVS0OVVWCgZZQhOC5{IHHu[EAxNjZizsznM41tNCCnYXPoJINwdXCjcnXkJJRwKHKnc4DlZ5RqfmViY3;ueJJwdHN? MonXN|A6QDR{N{i=

... Click to View More Cell Line Experimental Data

In vivo Chronic treatment with curcumin significantly reverses the CMS-induced behavioral abnormalities in stressed rats. Additionally, curcumin effectively inhibits cytokine gene expression at both the mRNA and the protein level and reduces the activation of NF-κB[4].

Protocol

Cell Research:

[5]

+ Expand
  • Cell lines: murine melanoma cell subline(B16-R)
  • Concentrations: 0-100 μM
  • Incubation Time: 24-48 h
  • Method:

    1×104 B16-R cells are cultivated as monolayer culture for 12 hr. They were then incubated in 200 μL of RPMI, 10% FBS containing curcumin at final concentrations from 1–100 μM in 96-multiwell plates for 24-48 hr. After these incubations, cells are washed twice in PBS and 500 μl of fresh culture medium containing MTT (0.3 mg/mL) are added for colorimetric assay.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: Female B6D2F1 mice (6-8 weeks old)
  • Formulation: Dissolved in DMSO and diluted with 0.85% NaCl
  • Dosages: 25 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 73 mg/mL (198.16 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
10% DMSO+50% PEG 300+ddH2O
For best results, use promptly after mixing.
40mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 368.38
Formula

C21H20O6

CAS No. 458-37-7
Storage powder
in solvent
Synonyms Diferuloylmethane

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02277223 Not yet recruiting Ulcerative Colitis Schneider Children''s Medical Center Israel June 1 2019 Phase 3
NCT02277223 Not yet recruiting Ulcerative Colitis Schneider Children''s Medical Center Israel June 1 2019 Phase 3
NCT03790605 Not yet recruiting Periodontitis KLE Society''s Institute of Dental Sciences April 1 2019 Phase 3
NCT03790605 Not yet recruiting Periodontitis KLE Society''s Institute of Dental Sciences April 1 2019 Phase 3
NCT03864783 Not yet recruiting Non-Alcoholic Fatty Liver Disease|Insulin Resistance|Glucose Tolerance Impaired|Obesity Abdominal Steno Diabetes Center|University of Copenhagen|University of Aarhus|Herlev and Gentofte Hospital March 2019 Not Applicable
NCT03865992 Recruiting Breast Cancer|Joint Pain City of Hope Medical Center|National Cancer Institute (NCI) March 28 2019 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Histone Acetyltransferase Signaling Pathway Map

Tags: buy Curcumin | Curcumin ic50 | Curcumin price | Curcumin cost | Curcumin solubility dmso | Curcumin purchase | Curcumin manufacturer | Curcumin research buy | Curcumin order | Curcumin mouse | Curcumin chemical structure | Curcumin mw | Curcumin molecular weight | Curcumin datasheet | Curcumin supplier | Curcumin in vitro | Curcumin cell line | Curcumin concentration | Curcumin nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID