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BTYNB

Synonyms: BTYNB IMP1 Inhibitor, MDK6620

BTYNB (BTYNB IMP1 Inhibitor, MDK6620) is a potent and selective inhibitor of IMP1 binding to c-Myc mRNA. BTYNB downregulates β-TrCP1 mRNA and reduces activation of nuclear transcriptional factors-kappa B (NF-κB). BTYNB disrupts this enhancer function by impairing IGF2 mRNA-binding protein 1 (IGF2BP1)-RNA association.

BTYNB Chemical Structure

BTYNB Chemical Structure

CAS: 304456-62-0

Purity & Quality Control

Batch: S987101 DMSO] 62 mg/mL] false] Ethanol] 4 mg/mL] false] Water] Insoluble] false Purity: 99.05%
99.05

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Biological Activity

Description BTYNB (BTYNB IMP1 Inhibitor, MDK6620) is a potent and selective inhibitor of IMP1 binding to c-Myc mRNA. BTYNB downregulates β-TrCP1 mRNA and reduces activation of nuclear transcriptional factors-kappa B (NF-κB). BTYNB disrupts this enhancer function by impairing IGF2 mRNA-binding protein 1 (IGF2BP1)-RNA association.
Targets
IMP1 [1] c-Myc [1] NF-κB [1]
In vitro
In vitro

In cells, BTYNB downregulates several mRNA transcripts regulated by IMP1. BTYNB destabilizes c-Myc mRNA, resulting in downregulation of c-Myc mRNA and protein. BTYNB downregulates β-TrCP1 mRNA and reduces activation of nuclear transcriptional factors-kappa B (NF-κB). The oncogenic translation regulator, eEF2, emerges as a new IMP1 target mRNA, enabling BTYNB to inhibit tumor cell protein synthesis. BTYNB potently inhibits proliferation of IMP1-containing ovarian cancer and melanoma cells with no effect in IMP1-negative cells. Overexpression of IMP1 reverses BTYNB inhibition of cell proliferation. BTYNB completely blocks anchorage-independent growth of melanoma and ovarian cancer cells in colony formation assays.[1]
Cell Research Cell lines SK-MEL2 cells
Concentrations 10 μM
Incubation Time 24 h
Method

For stability experiments, SK-MEL2 cells are treated with either DMSO or 10 μM BTYNB for 24 hours. mRNA synthesis is inhibited by treatment with 5 μM Actinomycin D (mRNA decay), and the cells are harvested at the indicated time points.

Chemical Information & Solubility

Molecular Weight 309.18 Formula

C12H9BrN2OS
CAS No. 304456-62-0 SDF --
Smiles NC(=O)C1=CC=CC=C1N=CC2=CC=C(Br)S2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 62 mg/mL ( (200.53 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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