Acetylcysteine (N-acetylcysteine)

Catalog No.S1623 Synonyms: NAC

For research use only.

Acetylcysteine (N-acetyl-l-cysteine, NAC) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions of Acetylcysteine are best fresh-prepared.

Acetylcysteine (N-acetylcysteine) Chemical Structure

CAS No. 616-91-1

Selleck's Acetylcysteine (N-acetylcysteine) has been cited by 106 publications

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Biological Activity

Description Acetylcysteine (N-acetyl-l-cysteine, NAC) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions of Acetylcysteine are best fresh-prepared.
Targets
NF-κB [8] Ferroptosis [9] ROS [6] TNF-α [7]
In vitro

N-acetylcysteine inhibits activation of c-Jun N-terminal kinase, p38 MAP kinase and redox-sensitive activating protein-1 and nuclear factor kappa B transcription factor activities regulating expression of numerous genes. N-acetylcysteine can also prevent apoptosis and promote cell survival by activating extracellular signal-regulated kinase pathway, a concept useful for treating certain degenerative diseases. N-acetylcysteine directly modifies the activity of several proteins by its reducing activity. [1] N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents death of PC12 cells and sympathetic neurons. [2] N-acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells. [3] N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it was suggested that NAC interferes with redox-sensitive steps in the NGF mechanism. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HUVEC cells NVfmfJBwTnWwY4Tpc44h[XO|YYm= M{HMT|Ex|ryP MoThNog> NF\iSmJJXV[HQ4Oge4Vz\SCycnX0doVifGWmIIfpeIghOTBizszNJG5CSyCob4KgNkBpd3W{czygZY5lKHSqZX6geJJm[XSnZDD3bZRpKDBwNTFOwG0hWEOEIEGxPE4LeHKnc3XueINmKG:oIF7BR{Bnd3JiNEigbI92enNw MoLTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUKxPVQoRjJ6NUmyNVk1RC:jPh?=
HUVEC cells M{DjSmZ2dmO2aX;uJIF{e2G7 NHLYN3kz|ryPLEVOwG0tQM7:TR?= NF24Zm06OG2rbh?= NEHFd5NJXV[HQ4OgeIhifCC5ZYLlJIV5eG:|ZXSge4l1cCC|ZYL1cUBw\iBiUD6g[oFt[2myYYL1cUBidmRidILlZZRm\CC5aYToJG5CSyB{INM1Ug+9lDRiwsXN89yNQCEEtV2uJHN2eGW{bnH0ZY51KG[{b32gZ5VtfHW{ZTDhcoQhdHm|ZXSgZ4VtdHNMY4XseJVz\SC5ZYLlJI1m[XO3cnXkJIZweiCKMl:yMEBIW0hiYX7kJG1FSSCuZY\lcJMv MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZyMke2N{c,OjF4MEK3OlM9N2F-
BL21 (DE3) MoHZSpVv[3Srb36gZZN{[Xl? M1XaT|MxKG2rboO= M3PE[mlvcGmkaYTpc44hd2ZiaHX4ZYhqe3SrZHnu[U11[WepZXSgTW1RNTdiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IIXzbY5oKG[udX;yc4NqdGyrbjDhd{B{fWK|dILheIUhcW6ldXLheIVlKG[xcjCzNEBucW6|IHL5JHRGS0GQIH\seY9z\XOlZX70JJBt[XSnIILlZYRmeiCjbnHsfZNqeyxiSVO1NEA:KDJyLkeg{txONg>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzNUWzNEc,OjV6MUW1N|A9N2F-
PC12 M4TndGN6fG:2b4jpZ4l1gSCjc4PhfS=> M{fibVI1KGi{cx?= M4DPV2lvcGmkaYTpc44hd2ZiNj3ofYRzd3i7ZH;wZY1qdmViaX7keYNm\CCleYTveI95cWOrdImgbY4hemG2IGDDNVIh[2WubIOgdJJmfHKnYYTl[EBnd3JiMkSgbJJ{KGG|c3Xzd4VlKGG|IHXs[ZZifGmxbjDv[kBqdnS{YXPlcIx2dGG{IHfseZRifGirb37lJIxmfmWu M1zD[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW4OFU1Lz5zN{G1PFQ2PDxxYU6=
PC12 MkXIR5l1d3SxeHnjbZR6KGG|c3H5 M3OzRVI1KGi{cx?= Mn3qTY5pcWKrdHnvckBw\iCqeXTyc4dmdiCyZYLvfIll\SCrbnT1Z4VlKGO7dH;0c5hq[2m2eTDpckBz[XRiUFOxNkBk\WyuczDwdoV1emWjdHXkJIZweiB{NDDodpMh[XO|ZYPz[YQh[XNiZXzleoF1cW:wIH;mJIlvfHKjY3XscJVt[XJiZ3z1eIF1cGmxbnWgcIV3\Wx? MnLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUi0OVQoRjF5MUW4OFU1RC:jPh?=
NHBE NX;2OXNnS3m2b4TvfIlkcXS7IHHzd4F6 NUjPUnVwOSCvTR?= NVL6[HJwOThiaILz MWjDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCrbjDOTGJGKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geGJJWC2rbnT1Z4VlKEeVSDDk[ZBt\XSrb36gZZQhOSCvTTDwdoVqdmO3YnH0[YQh\m:{IEG4JIhzeyCob3zsc5dm\CCkeTD0RmhRKGGmZHn0bY9vKG[xcjC0JIhzeyCkeTD0bIlwe3SjcjDkfYUh[mG|ZXSg[ox2d3Knc3PlcoNmKGG|c3H5 NGThcVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CzNVY4OCd-MkewN|E3PzB:L3G+
HUVEC M1HsdmFvfGmxeHnkZY51KGG|c3H5 MkK0OUBudW:uL1y= MV:0JIhzew>? MnO2RY51cW:6aXThcpQh[WO2aY\peJkh[WejaX7zeEBJOk9{LXnu[JVk\WRibHnwbYQh[WOldX31cIF1cW:wIHnuJGhWXkWFIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBOTEFibHX2[Ywh[XRiNTDtcY9tN0xiaX7jeYJifGWmIESgbJJ{KHC{aX;yJJRwKEh{T{KgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEGyJIhzew>? MmPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NEGyPFAoRjJ{OESxNlgxRC:jPh?=
HUVEC MX3BcpRqd3irZHHueEBie3OjeR?= NXTrXGFrPSCvbX;sM2w> M1T5VFQhcHK| MknBRY51cW:6aXThcpQh[WO2aY\peJkhcW5iSGXWSWMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFiyU|IucW6mdXPl[EBIW0hiYXP0bZZqfHliYYSgOUBudW:uL1ygbY5kfWKjdHXkJFQhcHK|IIDybY9zKHSxIFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFEzKGi{cx?= M1LBe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OESxNlgxLz5{Mki0NVI5ODxxYU6=
HUVEC NXjNZ4pQS3m2b4TvfIlkcXS7IHHzd4F6 Mn:wOUBudW:uL1y= NGLzdWo1KGi{cx?= NGPDU2JKdmirYnn0bY9vKG:oIFiyU|IucW6mdXPl[EBkgXSxdH;4bYNqfHliaX6gTHVXTUNiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[XRiNTDtcY9tN0xiaX7jeYJifGWmIESgbJJ{KHC{aX;yJJRwKEh{T{KgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEGyJIhzeyCkeTDNWHQh[XO|YYm= NGDr[ZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki0NVI5OCd-MkK4OFEzQDB:L3G+
HUVEC Mnn1R5l1d3SxeHnjbZR6KGG|c3H5 NXi2NJVZPSCvbX;sM2w> NYLlSlZZPCCqcoO= NIrrTFlKdmirYnn0bY9vKG:oIFiyU|IucW6mdXPl[EBkgXSxdH;4bYNqfHliaX6gTHVXTUNiY3XscJMh[XO|ZYPz[YQh[XNiTFTIJJJmdGWjc3WgZZQhPSCvbX;sM2whcW6ldXLheIVlKDRiaILzJJBzcW:{IITvJGgzVzJiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDF{IHjyd{BjgSCORFigZZN{[Xl? M3W0SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OESxNlgxLz5{Mki0NVI5ODxxYU6=
SH-SY5Y MVrO[ZVzd3C{b4TlZ5RqfmViYYPzZZk> M4nPUVUhdU1? M3\VclQhcHK| MVLO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliaX6gTFJQOi2|dHnteYxifGWmIHj1cYFvKFOKLWPZOXkh[2WubIOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJGJigCCneIDy[ZN{cW:wIHH0JFUhdU1iaX7jeYJifGWmIH\vdkA1KGi{czDwdolweiC2bzDINm8zKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAyOiCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[{BidmGueYPpdy=> Ml3vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEOwPFUoRjJ|NECzNFg2RC:jPh?=
SH-SY5Y MW\O[ZVzd3C{b4TlZ5RqfmViYYPzZZk> M3W3blUhdU1? M4faOlQhcHK| M{LwTG5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBJOk9{LYP0bY12dGG2ZXSgbJVu[W5iU1itV3k2YSClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCEY3yyJIV5eHKnc4Ppc44h[XRiNTDtUUBqdmO3YnH0[YQh\m:{IESgbJJ{KHC{aX;yJJRwKEh{T{KgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEGyJIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| Mn3nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEOwPFUoRjJ|NECzNFg2RC:jPh?=
HT22 NX3SNXlZVmW3cn;wdo91\WO2aY\lJIF{e2G7 NYey[pdYPSCvTR?= NIT6SXozPCCqcoO= NF7VTo9P\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDj[YxtKGSnYYToJIlvKG2xdYPlJGhVOjJiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYYSgOUBuVSCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6 NXLwUXVDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNlI1QDFpPkK5NVIzPDhzPD;hQi=>
HT22 NWfHNHZuVmW3cn;wdo91\WO2aY\lJIF{e2G7 NYO2ZVVnPTBidV2= NEC5U28yOCC2bzCxNkBpenN? NWHlOVU4VmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgZ4VtdCCmZXH0bEBqdiCvb4Xz[UBJXDJ{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBieG:ydH;0bYMh[2WubIOgZZQhPTBidV2gZYZ1\XJiMUCgeI8hOTJiaILzJIJ6KEGwbnX4bY4hXi2jbHX4ZUA1QDhxcILvdIllcXWvIHnv[Ill\SC|dHHpcolv\yCkYYPl[EBndG:5IHP5eI9u\XS{eR?= NW\1UnBzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNlI1QDFpPkK5NVIzPDhzPD;hQi=>
HL-7702 NUTNWJhsUGWyYYTvdJJwfGWldHn2[UBie3OjeR?= NGnTcogyOCC3TR?= MWGyOEBpenN? NYLJb|IyUGWyYYTvdJJwfGWldHn2[UBi[3Srdnn0fUBi\2GrboP0JGFRSVBvaX7keYNm\CClZXzsJIlvcnW{eTDpckBpfW2jbjDIUE04PzB{IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJJN2en[rdnHsJJJifGViYYSgNVAhfU1icILlMYlv[3WkYYTl[EBnd3JiMkSgbJJ{KGKnZn;y[UBCWEGSIHHk[Il1cW:wIHHu[EBu\WG|dYLl[EA3KGi{czDwc5N1KEGSQWCgZ4hidGynbnflJIJ6KE2WVDDhd5NigQ>? MmPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5MkmwOVYoRjJ6N{K5NFU3RC:jPh?=
In vivo

N-acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 163.19
Formula

C5H9NO3S

CAS No. 616-91-1
Storage 3 years -20°C powder
Smiles CC(=O)NC(CS)C(=O)O

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05287724 Recruiting Drug: N-acetyl cysteine|Drug: Placebo Pruritus|Skin Disorder Wright State University August 1 2022 Early Phase 1
NCT05241262 Recruiting Drug: N-Acetylcysteine Mitochondrial Disease Darryl C. De Vivo MD|United States Department of Defense|Columbia University June 2022 Phase 1
NCT05348993 Not yet recruiting Drug: G03-52-01|Drug: Placebo Healthy|Botulinum Toxin Ology Bioservices|United States Department of Defense June 15 2022 Phase 2
NCT05273619 Recruiting Drug: XC8 film-coated tablets 10 mg|Drug: Placebo Common Cold|Acute Respiratory Infection|Influenza Human Valenta Pharm JSC April 5 2022 Phase 3
NCT05122559 Recruiting Drug: N-acetyl cysteine|Drug: Placebo Multiple Sclerosis|Multiple Sclerosis Primary Progressive|Multiple Sclerosis Secondary Progressive Emmanuelle Waubant MD PhD|United States Department of Defense|University of California San Francisco February 16 2022 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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