Acetylcysteine (N-acetylcysteine)

Catalog No.S1623 Synonyms: NAC

For research use only.

Acetylcysteine (N-acetyl-l-cysteine, NAC) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions of Acetylcysteine are best fresh-prepared.

Acetylcysteine (N-acetylcysteine) Chemical Structure

CAS No. 616-91-1

Selleck's Acetylcysteine (N-acetylcysteine) has been cited by 81 publications

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Biological Activity

Description Acetylcysteine (N-acetyl-l-cysteine, NAC) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions of Acetylcysteine are best fresh-prepared.
Targets
NF-κB [8]
()
Ferroptosis [9]
()
ROS [6]
()
TNF-α [7]
()
In vitro

N-acetylcysteine inhibits activation of c-Jun N-terminal kinase, p38 MAP kinase and redox-sensitive activating protein-1 and nuclear factor kappa B transcription factor activities regulating expression of numerous genes. N-acetylcysteine can also prevent apoptosis and promote cell survival by activating extracellular signal-regulated kinase pathway, a concept useful for treating certain degenerative diseases. N-acetylcysteine directly modifies the activity of several proteins by its reducing activity. [1] N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents death of PC12 cells and sympathetic neurons. [2] N-acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells. [3] N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it was suggested that NAC interferes with redox-sensitive steps in the NGF mechanism. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HUVEC cells MmDDSpVv[3Srb36gZZN{[Xl? NGPzXoIyOM7:TR?= MVWybC=> M2nUO2hWXkWFczD3[ZJmKHC{ZYTy[YF1\WRid3n0bEAyOCEQvF2gUmFEKG[xcjCyJIhwfXK|LDDhcoQhfGinbjD0doVifGWmIIfpeIghOC53IN88UUBRS0JiMUG4MipxemW|ZX70Z4Uhd2ZiTlHDJIZweiB2ODDoc5Vzey5? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV7MkG5OEc,Ojh3OUKxPVQ9N2F-
HUVEC cells MoTXSpVv[3Srb36gZZN{[Xl? NGDsbHIz|ryPLEVOwG0tQM7:TR?= MWW5NI1qdg>? NFvUN4xJXV[HQ4OgeIhifCC5ZYLlJIV5eG:|ZXSge4l1cCC|ZYL1cUBw\iBiUD6g[oFt[2myYYL1cUBidmRidILlZZRm\CC5aYToJG5CSyB{INM1Ug+9lDRiwsXN89yNQCEEtV2uJHN2eGW{bnH0ZY51KG[{b32gZ5VtfHW{ZTDhcoQhdHm|ZXSgZ4VtdHNMY4XseJVz\SC5ZYLlJI1m[XO3cnXkJIZweiCKMl:yMEBIW0hiYX7kJG1FSSCuZY\lcJMv NETOc2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[wNlc3Oyd-MkG2NFI4PjN:L3G+
BL21 (DE3) NFy4dZhHfW6ldHnvckBie3OjeR?= NVvXNmdPOzBibXnudy=> M{L4emlvcGmkaYTpc44hd2ZiaHX4ZYhqe3SrZHnu[U11[WepZXSgTW1RNTdiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IIXzbY5oKG[udX;yc4NqdGyrbjDhd{B{fWK|dILheIUhcW6ldXLheIVlKG[xcjCzNEBucW6|IHL5JHRGS0GQIH\seY9z\XOlZX70JJBt[XSnIILlZYRmeiCjbnHsfZNqeyxiSVO1NEA:KDJyLkeg{txONg>? M1\qeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEG1OVMxLz5{NUixOVU{ODxxYU6=
PC12 MXTDfZRwfG:6aXPpeJkh[XO|YYm= MVyyOEBpenN? MlHPTY5pcWKrdHnvckBw\iB4LXj5[JJwgHmmb4DhcYlv\SCrbnT1Z4VlKGO7dH;0c5hq[2m2eTDpckBz[XRiUFOxNkBk\WyuczDwdoV1emWjdHXkJIZweiB{NDDodpMh[XO|ZYPz[YQh[XNiZXzleoF1cW:wIH;mJIlvfHKjY3XscJVt[XJiZ3z1eIF1cGmxbnWgcIV3\Wx? NUTXXY9TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexOVg1PTRpPkG3NVU5PDV2PD;hQi=>
PC12 NF3NfWhEgXSxdH;4bYNqfHliYYPzZZk> NVXy[GNzOjRiaILz NUXJfndjUW6qaXLpeIlwdiCxZjDofYRzd2enbjDw[ZJwgGmmZTDpcoR2[2WmIHP5eI91d3irY3n0fUBqdiC{YYSgVGMyOiClZXzsd{BxemW2cnXheIVlKG[xcjCyOEBpenNiYYPz[ZN{\WRiYYOg[YxmfmG2aX;uJI9nKGmwdILhZ4VtdHWuYYKg[4x2fGG2aHnvcoUhdGW4ZXy= NUHjfYlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexOVg1PTRpPkG3NVU5PDV2PD;hQi=>
NHBE Mn\kR5l1d3SxeHnjbZR6KGG|c3H5 MYexJI1O NYHucFZwOThiaILz NIG2OY9EgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBPUEKHIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhfEKKUD3pcoR2[2WmIFfTTEBl\XCuZYTpc44h[XRiMTDtUUBxemWrbnP1ZoF1\WRiZn;yJFE5KGi{czDmc4xtd3enZDDifUB1SkiSIHHk[Il1cW:wIH\vdkA1KGi{czDifUB1cGmxc4ThdkBlgWViYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6 M4O2T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEOxOlcxLz5{N{CzNVY4ODxxYU6=
HUVEC NHqwNFJCdnSrb4jp[IFvfCCjc4PhfS=> NHy2fIQ2KG2vb3yvUC=> NWH2bos4PCCqcoO= NHPheJdCdnSrb4jp[IFvfCCjY4Tpeol1gSCjZ3HpcpN1KEh{T{KtbY5lfWOnZDDsbZBq\CCjY3P1cZVt[XSrb36gbY4hUFWYRVOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKE2GQTDs[ZZmdCCjdDC1JI1ud2xxTDDpcoN2[mG2ZXSgOEBpenNicILpc5IhfG9iSELPNkBkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVIhcHK| M1OyO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OESxNlgxLz5{Mki0NVI5ODxxYU6=
HUVEC M1jWRWFvfGmxeHnkZY51KGG|c3H5 MWS1JI1ud2xxTB?= MXu0JIhzew>? MlHyRY51cW:6aXThcpQh[WO2aY\peJkhcW5iSGXWSWMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFiyU|IucW6mdXPl[EBIW0hiYXP0bZZqfHliYYSgOUBudW:uL1ygbY5kfWKjdHXkJFQhcHK|IIDybY9zKHSxIFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFEzKGi{cx?= M1Pa[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OESxNlgxLz5{Mki0NVI5ODxxYU6=
HUVEC MnrFR5l1d3SxeHnjbZR6KGG|c3H5 MlTHOUBudW:uL1y= NH\rWIo1KGi{cx?= NUTlT3JrUW6qaXLpeIlwdiCxZjDINm8zNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHnuJGhWXkWFIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGG2IEWgcY1wdC:OIHnuZ5Vj[XSnZDC0JIhzeyCycnnvdkB1dyCKMl:yJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjCxNkBpenNiYomgUXRVKGG|c3H5 NIfRXIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki0NVI5OCd-MkK4OFEzQDB:L3G+
HUVEC M1G4RmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXX4flZMPSCvbX;sM2w> NWKw[|IxPCCqcoO= NFTafZdKdmirYnn0bY9vKG:oIFiyU|IucW6mdXPl[EBkgXSxdH;4bYNqfHliaX6gTHVXTUNiY3XscJMh[XO|ZYPz[YQh[XNiTFTIJJJmdGWjc3WgZZQhPSCvbX;sM2whcW6ldXLheIVlKDRiaILzJJBzcW:{IITvJGgzVzJiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDF{IHjyd{BjgSCORFigZZN{[Xl? NFvoPWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki0NVI5OCd-MkK4OFEzQDB:L3G+
SH-SY5Y M3LoRm5mfXKxcILveIVkfGm4ZTDhd5NigQ>? NUPz[nFiPSCvTR?= NFvmfHM1KGi{cx?= M3vZUW5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBJOk9{LYP0bY12dGG2ZXSgbJVu[W5iU1itV3k2YSClZXzsd{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4YhSmG6IHX4dJJme3Orb36gZZQhPSCvTTDpcoN2[mG2ZXSg[o9zKDRiaILzJJBzcW:{IITvJGgzVzJiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ MlvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEOwPFUoRjJ|NECzNFg2RC:jPh?=
SH-SY5Y M3q1Um5mfXKxcILveIVkfGm4ZTDhd5NigQ>? MVi1JI1O NFvZbZE1KGi{cx?= M3u2XG5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBJOk9{LYP0bY12dGG2ZXSgbJVu[W5iU1itV3k2YSClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCEY3yyJIV5eHKnc4Ppc44h[XRiNTDtUUBqdmO3YnH0[YQh\m:{IESgbJJ{KHC{aX;yJJRwKEh{T{KgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEGyJIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| NITkXnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwN|A5PSd-MkO0NFMxQDV:L3G+
HT22 M1jQSm5mfXKxcILveIVkfGm4ZTDhd5NigQ>? M3f0b|UhdU1? NFrEdFEzPCCqcoO= NYjwTGFiVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgZ4VtdCCmZXH0bEBqdiCvb4Xz[UBJXDJ{IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHH0JFUhdU1iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>? M2PUPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUKyOFgyLz5{OUGyNlQ5OTxxYU6=
HT22 Ml\RUoV2em:ycn;0[YN1cX[nIHHzd4F6 MX:1NEB2VQ>? MkTXNVAhfG9iMUKgbJJ{ NGDtSoRP\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDj[YxtKGSnYYToJIlvKG2xdYPlJGhVOjJiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIFxd3C2b4TpZ{Bk\WyuczDheEA2OCC3TTDh[pRmeiBzMDD0c{AyOiCqcoOgZpkhSW6wZYjpckBXNWGuZYjhJFQ5QC:ycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oKGKjc3XkJIZtd3diY4n0c41mfHK7 NIL1eZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGyNlQ5OSd-MkmxNlI1QDF:L3G+
HL-7702 MX7I[ZBifG:ycn;0[YN1cX[nIHHzd4F6 M1XMUFExKHWP M4jwblI1KGi{cx?= M1XBT2hmeGG2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDBVGFRNWmwZIXj[YQh[2WubDDpcop2enliaX6gbJVu[W5iSFytO|cxOiClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDzeZJ3cX[jbDDyZZRmKGG2IEGwJJVOKHC{ZT3pcoN2[mG2ZXSg[o9zKDJ2IHjyd{Bj\W[xcnWgRXBCWCCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgOkBpenNicH;zeEBCWEGSIHPoZYxt\W6pZTDifUBOXFRiYYPzZZk> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd{OUC1Okc,Ojh5MkmwOVY9N2F-
In vivo

N-acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 33 mg/mL
(202.21 mM)
Water 33 mg/mL
(202.21 mM)
Ethanol '33 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 163.19
Formula

C5H9NO3S

CAS No. 616-91-1
Storage 3 years -20°C powder
Smiles CC(=O)NC(CS)C(=O)O

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05122559 Not yet recruiting Drug: N-acetyl cysteine|Drug: Placebo Multiple Sclerosis|Multiple Sclerosis Primary Progressive|Multiple Sclerosis Secondary Progressive Emmanuelle Waubant|United States Department of Defense|University of California San Francisco January 2022 Phase 2
NCT05123365 Recruiting Drug: N-Acetylcysteine Myeloproliferative Neoplasm|MPN|Essential Thrombocythemia|Polycythemia Vera|Myelofibrosis University of California Irvine January 3 2022 Phase 1|Phase 2
NCT04520139 Not yet recruiting Drug: N-Acetyl-Cysteine|Other: Placebo Ovarian Cancer|Cognitive Impairment University of California Irvine|Jarrow Formulas Inc January 2022 Phase 1|Phase 2
NCT05141279 Not yet recruiting -- Breast Cancer Assiut University December 1 2021 --
NCT05089981 Not yet recruiting Drug: N acetyl cysteine|Drug: Placebo of N acetyl cysteine Acute on Chronic Liver Failure(ACLF) Aga Khan University|National Institute of Liver & GI Diseases Pakistan November 1 2021 Not Applicable
NCT05146804 Recruiting Other: BroccoCress|Other: Affilla Cress Inflammation Maastricht University Medical Center November 11 2021 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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