For research use only.
Molecular Weight(MW): 211.15
Sodium 4-Aminosalicylate is an antibiotic used to treat tuberculosis via NF-κB inhibition and free radical scavenging.
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A. Raw264.7 cells were pretreated with NAC (1 mM), SB202190 (10 μM) and Sodium 4-aminosalicylate (10 μM), respectively, for 30 min prior to 10 μg/ml ConA treatment for 24 h. Cell viability was measured by an MTT assay and ROS production detected by H2DCF-DA.
PLoS One, 2016, 11(3):e0149754.. Sodium 4-Aminosalicylate purchased from Selleck.
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|Description||Sodium 4-Aminosalicylate is an antibiotic used to treat tuberculosis via NF-κB inhibition and free radical scavenging.|
|Features||4-Aminosalicylate is considered to be the active moiety of sulfasalazine.|
4-Aminosalicylate reacts promptly with DPPH, suggesting a potent radical scavenger activity. 4-Aminosalicylate exhibits peroxyl radical scavenging activity generated by the water-soluble 2,2'-azobis-(2-amidinopropane hydrochloride) azoinitiator of peroxyl radicals, as evidenced by the inhibition of cis-parinaric acid fluorescence decay or oxygen consumption. 4-Aminosalicylate rapidly scavenges peroxyl radicals in the aqueous phase, producing a concentration-dependent inhibition period similar to Trolox or cysteine, suggesting an antioxidant activity of chain-breaking type.  [14C]4-Aminosalicylate transforms to a number of metabolites, among which we have characterized salicylate and gentisate, in activated mononuclear cells and activated granulocytes. 4-Aminosalicylate (0.65 mM) diminishes the lethal effect on cultured Chinese hamster ovary cells of adding either superoxide radical or hydrogen peroxide.  Aminosalicylate (25 mM) stimulates phospholipase D in a time- and concentration-dependent manner via a pathway involving inositol 1,4,5-trisphosphate generation, calcium fluxes, and Gi/Go in cultured mouse peritoneal macrophages. 4-aminosalicylate (20 mM) increases the levels of inositol 1,4,5-trisphosphate by 260% after treatment of macrophages. 4-aminosalicylate (5 mM) potentiates the activation of PLD by protein kinase C in cultured mouse peritoneal macrophages.  4-aminosalicylate (0.1 mM) decreases the LTB4 synthesis in a dose-related fashion in isolated colonic mucosal cells, thus diminishes the LTB4/PGE2 ratio.  4-aminosalicylate (0.1 mg/mL) is preferentially transported in the basolateral (BL) to apical (AP) direction, and the N-acetyl metabolite appeared only in the AP compartment. 
|In vivo||4-aminosalicylate (7.5 mg/mL, regional perfusions) results in the appearance of N-acetyl-5-aminosalicylic acid in the intestinal lumen in the anesthetized rat. |
-  Dinis TC, et al. Arch Biochem Biophys, 1994, 315(1), 161-169.
-  Dull BJ, et al. Biochem Pharmacol, 1987, 36(15), 2467-2472.
-  Gómez-Muñoz A, et al. Biochim Biophys Acta, 2001, 1533(2), 110-118.
|In vitro||DMSO||42 mg/mL (198.91 mM)|
|Water||42 mg/mL (198.91 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
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