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CAS No. 6018-19-5
Sodium 4-Aminosalicylate is an antibiotic used to treat tuberculosis via NF-κB inhibition and free radical scavenging.
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A. Raw264.7 cells were pretreated with NAC (1 mM), SB202190 (10 μM) and Sodium 4-aminosalicylate (10 μM), respectively, for 30 min prior to 10 μg/ml ConA treatment for 24 h. Cell viability was measured by an MTT assay and ROS production detected by H2DCF-DA.
PLoS One, 2016, 11(3):e0149754.. Sodium 4-Aminosalicylate purchased from Selleck.
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|Description||Sodium 4-Aminosalicylate is an antibiotic used to treat tuberculosis via NF-κB inhibition and free radical scavenging.|
|Features||4-Aminosalicylate is considered to be the active moiety of sulfasalazine.|
4-Aminosalicylate reacts promptly with DPPH, suggesting a potent radical scavenger activity. 4-Aminosalicylate exhibits peroxyl radical scavenging activity generated by the water-soluble 2,2'-azobis-(2-amidinopropane hydrochloride) azoinitiator of peroxyl radicals, as evidenced by the inhibition of cis-parinaric acid fluorescence decay or oxygen consumption. 4-Aminosalicylate rapidly scavenges peroxyl radicals in the aqueous phase, producing a concentration-dependent inhibition period similar to Trolox or cysteine, suggesting an antioxidant activity of chain-breaking type.  [14C]4-Aminosalicylate transforms to a number of metabolites, among which we have characterized salicylate and gentisate, in activated mononuclear cells and activated granulocytes. 4-Aminosalicylate (0.65 mM) diminishes the lethal effect on cultured Chinese hamster ovary cells of adding either superoxide radical or hydrogen peroxide.  Aminosalicylate (25 mM) stimulates phospholipase D in a time- and concentration-dependent manner via a pathway involving inositol 1,4,5-trisphosphate generation, calcium fluxes, and Gi/Go in cultured mouse peritoneal macrophages. 4-aminosalicylate (20 mM) increases the levels of inositol 1,4,5-trisphosphate by 260% after treatment of macrophages. 4-aminosalicylate (5 mM) potentiates the activation of PLD by protein kinase C in cultured mouse peritoneal macrophages.  4-aminosalicylate (0.1 mM) decreases the LTB4 synthesis in a dose-related fashion in isolated colonic mucosal cells, thus diminishes the LTB4/PGE2 ratio.  4-aminosalicylate (0.1 mg/mL) is preferentially transported in the basolateral (BL) to apical (AP) direction, and the N-acetyl metabolite appeared only in the AP compartment. 
|In vivo||4-aminosalicylate (7.5 mg/mL, regional perfusions) results in the appearance of N-acetyl-5-aminosalicylic acid in the intestinal lumen in the anesthetized rat. |
-  Dinis TC, et al. Arch Biochem Biophys, 1994, 315(1), 161-169.
-  Dull BJ, et al. Biochem Pharmacol, 1987, 36(15), 2467-2472.
-  Gómez-Muñoz A, et al. Biochim Biophys Acta, 2001, 1533(2), 110-118.
|In vitro||DMSO||42 mg/mL (198.91 mM)|
|Water||42 mg/mL (198.91 mM)|
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