Dihydroartemisinin (DHA)

Catalog No.S2290 Synonyms: Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol

For research use only.

Dihydroartemisinin (DHA, Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.

Dihydroartemisinin (DHA) Chemical Structure

CAS No. 71939-50-9

Selleck's Dihydroartemisinin (DHA) has been cited by 16 publications

Purity & Quality Control

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Biological Activity

Description Dihydroartemisinin (DHA, Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.
Targets
NF-κB [4]
()
In vitro

Dihydroartemisinin (DHA) inhibits the growth of certain cancer cell lines and xenograft tumors such as leukemia, glioma, fibrosarcoma, and breast, cervical, ovarian, lung, oral and pancreatic cancer. DHA inhibits cell and tumor growth by modulating various tumor-suppressive pathways, such as inhibiting cell proliferation and inducing apoptosis through regulation of proliferation- and apoptosis-related proteins.DHA inhibits the proliferation and viability of cells in a dose-dependent manner and induces apoptosis.DHA-mediated cytotoxicity is tumor selective. The endoperoxide bridge of DHA is reportedly essential for its cytotoxicity because it reacts with intracellular ferrous iron to generate reactive oxygen species or carbon-centered radicals, leading to cytotoxicity[1].

Assay
Methods Test Index PMID
Western blot p-STAT3 / p-JAK2 ; MMP-2 / MMP-9 ; E-cadherin / N-cadherin / Vimentin / Twist / Slug / Snail 29434895 29472793 29387451
Immunofluorescence PDGFRα 29387451
Growth inhibition assay Cell viability 29114376
In vivo

DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis[2]. DHA has been shown in the rat whole embryo culture (WEC) to primarily affect primitive red blood cells (RBCs) causing subsequent tissue damage and dysmorphogenesis[3].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: pancreatic cancer cell line BxPc3-RFP
  • Concentrations: 2.5, 10, 40, or 80 μM
  • Incubation Time: 24, 48, and 72 h
  • Method:

    BxPc3-RFP cells (3.5×104cells/well) were seeded in poly D-lysine-coated black, μClear 96-well plates with 0.2 ml medium. After 24 h, the cells were treated with dimethyl sulfoxide (DMSO) (control) or different concentrations (2.5, 10, 40, or 80 μM) of DHA dissolved in DMSO for 24, 48, and 72 h. At each time point, the fluorescence intensity emitted from cells was measured.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 56 mg/mL warmed
(196.94 mM)
Ethanol 11 mg/mL warmed
(38.68 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.35
Formula

C15H24O5

CAS No. 71939-50-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CCC2C(C(OC3C24C1CCC(O3)(OO4)C)O)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03402789 Not yet recruiting Drug: Docosahexaenoic Acid|Drug: Placebo Oral Tablet Amblyopia Johns Hopkins University January 1 2022 Phase 1|Phase 2
NCT05071690 Not yet recruiting -- DHA as Antioxidant Anti-inflammatory Immunomodulation Overcome Cytokine Storm Ministry of Health Saudi Arabia|Faculty of medicine kafr elshiekh university November 2021 --
NCT04965129 Not yet recruiting Drug: Placebo|Drug: Fish oil Lung Cancer Universidade Federal do Rio de Janeiro|Institutos Madrileño de Estudios Avanzados IMDEA|Oncoclínicas September 1 2021 Not Applicable
NCT05128240 Recruiting Dietary Supplement: Placebo|Dietary Supplement: Ceto 10 Skin Health Epax Norway AS|Møreforsking AS August 23 2021 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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