Dihydroartemisinin (DHA)

Catalog No.S2290

Dihydroartemisinin (DHA) Chemical Structure

Molecular Weight(MW): 284.35

Dihydroartemisinin (DHA) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua.

Size Price Stock Quantity  
In DMSO USD 160 In stock
USD 120 In stock
USD 180 In stock
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Cited by 6 Publications

5 Customer Reviews

  • (D) Western Blot analysis of TCTP in cell lysates of MDA cells after 24, 48 and 72 h of exposition to DHA. β-actin was used as loading control.

    Oncotarget, 2015, 6(7):5275-91.. Dihydroartemisinin (DHA) purchased from Selleck.

    In the migration assays, the TCTP-positive cell lines NOZ, GBC-SD, and OCUG-1, and the TCTP-negative cell lines EH-GB-2 and SGC-996 were pre-treated with either vehicle or DHA (40 μM) for 2 days and then seeded in transwell plates for 24 h.

    J Exp Clin Cancer Res, 2017, 36(1):68. Dihydroartemisinin (DHA) purchased from Selleck.

  • Overexpression of GLUT1 inhibits cell death triggered by DHA. A549 and PC-9 cells were transfected with control (Ctr) and GLUT1 vector for 8 h respectively, and then treated with the indicated concentration of DHA for 48 h. (A) Cell lysates were subjected to western blot analysis with the indicated antibodies. (B) Cell growth inhibition activity was assessed by MTT. This experiment was repeated thrice. Columns, mean; bars, SD. ***, P < 0.001.

    PLoS One, 2015, 10(3):e0120426.. Dihydroartemisinin (DHA) purchased from Selleck.

    H1975 and A549 cells were treated with increasing concentration of DHA for 1 day (left) or with 15 μM DHA for up to 24 h (right). Mcl-1 expression was examined by Western blotting.

    Biochem Pharmacol, 2018, 150:72-85. Dihydroartemisinin (DHA) purchased from Selleck.

  • Effects of various concentrations of DHA (5, 10, 20, 50 and 100 µM) on NCI-H1975 cell viability.

    Mol Med Rep, 2017, 16(3):3475-3481. Dihydroartemisinin (DHA) purchased from Selleck.

Purity & Quality Control

Biological Activity

Description Dihydroartemisinin (DHA) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua.
In vitro

Dihydroartemisinin (DHA) inhibits the growth of certain cancer cell lines and xenograft tumors such as leukemia, glioma, fibrosarcoma, and breast, cervical, ovarian, lung, oral and pancreatic cancer. DHA inhibits cell and tumor growth by modulating various tumor-suppressive pathways, such as inhibiting cell proliferation and inducing apoptosis through regulation of proliferation- and apoptosis-related proteins.DHA inhibits the proliferation and viability of cells in a dose-dependent manner and induces apoptosis.DHA-mediated cytotoxicity is tumor selective. The endoperoxide bridge of DHA is reportedly essential for its cytotoxicity because it reacts with intracellular ferrous iron to generate reactive oxygen species or carbon-centered radicals, leading to cytotoxicity[1].

In vivo DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis[2]. DHA has been shown in the rat whole embryo culture (WEC) to primarily affect primitive red blood cells (RBCs) causing subsequent tissue damage and dysmorphogenesis[3].

Protocol

Cell Research:[1]
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  • Cell lines: pancreatic cancer cell line BxPc3-RFP
  • Concentrations: 2.5, 10, 40, or 80 μM
  • Incubation Time: 24, 48, and 72 h
  • Method: BxPc3-RFP cells (3.5×104cells/well) were seeded in poly D-lysine-coated black, μClear 96-well plates with 0.2 ml medium. After 24 h, the cells were treated with dimethyl sulfoxide (DMSO) (control) or different concentrations (2.5, 10, 40, or 80 μM) of DHA dissolved in DMSO for 24, 48, and 72 h. At each time point, the fluorescence intensity emitted from cells was measured.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 56 mg/mL warmed (196.94 mM)
Ethanol 11 mg/mL warmed (38.68 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.35
Formula

C15H24O5

CAS No. 71939-50-9
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03402789 Not yet recruiting Drug: Docosahexaenoic Acid|Drug: Placebo Oral Tablet Amblyopia Johns Hopkins University January 1 2020 Phase 1|Phase 2
NCT03937206 Active not recruiting Dietary Supplement: NutriterraTM|Dietary Supplement: Placebo Healthy Nuseed Americas Inc.|Nutrasource Diagnostics Inc. May 1 2019 Phase 1|Phase 2
NCT03576989 Recruiting Dietary Supplement: EPA+DHA|Other: placebo Chronic Venous Leg Ulcers Ohio State University|National Institute on Aging (NIA) April 15 2019 Not Applicable

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID