Home Immunology & Inflammation TLR agonist - NF-κB activator - TNF-alpha activator - Interleukins activator - NOS activator CU-T12-9 For research use only.

CU-T12-9

CU-T12-9 is a specific agonist of Toll-like receptor (TLR) 1/2 with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS.

CU-T12-9 Chemical Structure

CU-T12-9 Chemical Structure

CAS: 1821387-73-8

Selleck's CU-T12-9 has been cited by 2 publications

Purity & Quality Control

Batch: S297901 DMSO] 72 mg/mL] false] Ethanol] 5 mg/mL] false] Water] Insoluble] false Purity: 99.86%
99.86

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Biological Activity

Description CU-T12-9 is a specific agonist of Toll-like receptor (TLR) 1/2 with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS.
Targets
TLR 1/2 [1]
(HEK-Blue hTLR2 SEAP assay)
52.9 nM(EC50)
In vitro
In vitro

CU-T12-9 specifically induces TLR1/2 activation, and facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream NF-κB signaling.[1]
Cell Research Cell lines HEK-Blue hTLR2 cells
Concentrations 60 nM
Incubation Time 24 h
Method

Cells were cultured in 200 μl of DMEM supplemented with 10% FBS, 10× penicillin/streptomycin, and 10× l-glutamine.  Cells were implanted in 96-well plates (4 × 10<sup>4</sup> cells per well) for 24 hours at 37°C before drug treatment.  In the next 24 hours of treatment, medium was removed from the 96-well plate and replaced with 200 μl of supplemented Opti-MEM [0.5% FBS, penicillin (50 U/ml), streptomycin (50 μg/ml), 1× nonessential amino acids] containing 60 nM CU-T12-9, 0.66 nM (1 ng/ml) Pam3CSK4, or 0.77 nM (1 ng/ml) Pam2CSK4 according to the manufacturer’s protocol (InvivoGen), as well as different antibodies (0 to 10 μg/ml), including anti-hTLR1–IgG, anti-hTLR2–IgA, or anti-hTLR6–IgA. A sample buffer (20 μl) from each well of the cell culture supernatants was collected and transferred to a transparent 96-well plate.  Each well was treated with 200 μl of QUANTI-Blue buffer and incubated at 37°C for 1 h.

Chemical Information & Solubility

Molecular Weight 362.31 Formula

C17H13F3N4O2
CAS No. 1821387-73-8 SDF --
Smiles CNC1=C(C=C(C=C1)[N+]([O-])=O)[N]2C=NC(=C2)C3=CC=C(C=C3)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 72 mg/mL ( (198.72 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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