CU-T12-9

Catalog No.S2979

For research use only.

CU-T12-9 is a specific agonist of Toll-like receptor (TLR) 1/2 with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS.

CU-T12-9 Chemical Structure

CAS No. 1821387-73-8

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Biological Activity

Description CU-T12-9 is a specific agonist of Toll-like receptor (TLR) 1/2 with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS.
Targets
TLR 1/2 [1]
(HEK-Blue hTLR2 SEAP assay)
52.9 nM(EC50)
In vitro

CU-T12-9 specifically induces TLR1/2 activation, and facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream NF-κB signaling.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HEK-Blue hTLR2 cells
  • Concentrations: 60 nM
  • Incubation Time: 24 h
  • Method:

    Cells were cultured in 200 μl of DMEM supplemented with 10% FBS, 10× penicillin/streptomycin, and 10× l-glutamine.  Cells were implanted in 96-well plates (4 × 10<sup>4</sup> cells per well) for 24 hours at 37°C before drug treatment.  In the next 24 hours of treatment, medium was removed from the 96-well plate and replaced with 200 μl of supplemented Opti-MEM [0.5% FBS, penicillin (50 U/ml), streptomycin (50 μg/ml), 1× nonessential amino acids] containing 60 nM CU-T12-9, 0.66 nM (1 ng/ml) Pam3CSK4, or 0.77 nM (1 ng/ml) Pam2CSK4 according to the manufacturer’s protocol (InvivoGen), as well as different antibodies (0 to 10 μg/ml), including anti-hTLR1–IgG, anti-hTLR2–IgA, or anti-hTLR6–IgA. A sample buffer (20 μl) from each well of the cell culture supernatants was collected and transferred to a transparent 96-well plate.  Each well was treated with 200 μl of QUANTI-Blue buffer and incubated at 37°C for 1 h.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 362.31
Formula

C17H13F3N4O2

CAS No. 1821387-73-8
Storage 3 years -20°C powder
2 years -80°C in solvent

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