Catalog No.S7351

JSH-23 Chemical Structure

Molecular Weight(MW): 240.34

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.

Size Price Stock Quantity  
USD 147 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 13 Publications

6 Customer Reviews

  • CD10+GPR77+ CAFs were transduced without (-) or with GFP-shRNA or p65 shRNA or pretreated with inhibitors of NF-kB nuclear translocation. Quantitation of the percentage of CD10+GPR77+ CAFs (D) and the IL-6 and IL-8 levels (E) were shown.

    Cell, 2018, 172(4):841-856. JSH-23 purchased from Selleck.

    The population of cmyb1kdrl1 cells in Tg(cmyb:GFP/kdrl:mCherry) embryos was decreased in JSH-23–treated embryos. White arrowheads mark cmyb1kdrl1 cells in the AGM region at 36 hpf. Right panel, Quantification of cmyb1kdrl1 cells.

    Blood, 2015, 125(7): 1098-106 . JSH-23 purchased from Selleck.

  • J Mol Med (Berl), 2018, 96(3-4):301-313. JSH-23 purchased from Selleck.

    Pancreatic acini exposed for 1 h to 300 μM of oleic acid (OA) or linoleic acid (LA) were pre-treated for 45 min with the vehicle (DMSO), JSH-23 (30 μM) or SH-4-54 (10 μM). CCL2 mRNA expression was analyzed by qRT-PCR with 18S as internal standard. *p<0.05, **p<0.01 as compared with untreated acini, ♦♦p<0.01 as compared with OA- or LA-treated acini.

    Biochim Biophys Acta, 2015, 1852(12):2671-7. JSH-23 purchased from Selleck.

  • Effects of JSH-23 and pyrrolidinedithiocarbamic acid (PDTC) on LPS-induced p38 MAPK phosphorylation. After pretreatment with JSH-23 (10 lM) or PDTC (10 lM) for 30 min, cells were treated by LPS for 30 min before protein detection using western blot.

    J Neurochem, 2017, 140(4):589-604. JSH-23 purchased from Selleck.

    Suppression of NF-κB activity by JSH-23 attenuated HMGB1 induced active and latent HPSE expression. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.

    Am J Transl Res, 2016, 8(5):1971-84.. JSH-23 purchased from Selleck.

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro

JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]

In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]


Kinase Assay:[1]
+ Expand

Measurement of NF-κB transcriptional activity:

Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
Cell Research:[1]
+ Expand
  • Cell lines: RAW 264.7 cells
  • Concentrations: ~300 μM
  • Incubation Time: 24 hours
  • Method: Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: STZ-induced diabetic rats
  • Formulation: 0.5% sodium carboxymethyl cellulose
  • Dosages: ~3 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 240.34


CAS No. 749886-87-1
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

NF-κB Signaling Pathway Map

Related NF-κB Products0

Tags: buy JSH-23 | JSH-23 supplier | purchase JSH-23 | JSH-23 cost | JSH-23 manufacturer | order JSH-23 | JSH-23 distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID