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Catalog No.S7351

84 publications

JSH-23 Chemical Structure

CAS No. 749886-87-1

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.

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10mM (1mL in DMSO) USD 147 In stock
USD 147 In stock
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Selleck's JSH-23 has been cited by 84 publications

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Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro

JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HK-2 cells Mk[zSpVv[3Srb36gZZN{[Xl? Ml7zNVAx6oDLzszN MWmz5qCKcA>? MXrwdoV1emWjdH3lcpQhf2m2aDDKV2guOjNiZX\m[YN1cX[nbImgZoxw[2unZDDBcochUUlvaX7keYNm\CCwdXPs[YFzKHB4NTDhZ4N2dXWuYYTpc44> NX7idVE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C4O|Q2PDRpPkOwPFc1PTR2PD;hQi=>
U2OS/DR-GFP cells MWXGeY5kfGmxbjDhd5NigQ>? M3LIbVExKGGwZDCyNEDDvU1? M{nwVWpUUC1{MzD0doVifG2nboSgd4lodmmoaXPhcpRtgSCmZXPy[YF{\WRidHjlJGhTKG[{ZYH1[Y5kgQ>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODd5MEmyOEc,OzB5N{C5NlQ9N2F-
MCF-7:5C NX2xZmtHTnWwY4Tpc44h[XO|YYm= NWHifVVbOjBizsztc4wwVA>? NWjrZ2JMOyCjbnSgOkBl[Xm| MmK5TnNJNTJ|IHPvcZBt\XSnbImgZoxw[2unZDDFNk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCPQ1[tO|o2SyClZXzsdy=> M3LnVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkK0OFMxLz5|MEKyOFQ{ODxxYU6=
MCF-7:2A MUnGeY5kfGmxbjDhd5NigQ>? NWTkPW9XOjBizsztc4wwVA>? MUGzJIFv\CB4IHThfZM> MlfzTnNJNTJ|IHnuZ5Jm[XOnZDDFNk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCPQ1[tO|ozSSClZXzsdy=> NFLjbVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKyOFQ{OCd-M{CyNlQ1OzB:L3G+
BMDM MkTLSpVv[3Srb36gZZN{[Xl? MoPqOlAh|ryP NUL2cVZNOTlvMkSgbC=> MVjKV2guOjNiKE[wJO69VSliaX7keYNm\CCjIHTlZ5Jm[XOnIH;mJGlNNTIQsjDy[Yxm[XOn M1TBblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUO4N|IyLz5|MEGzPFMzOTxxYU6=
HL60 cells M{L2fGZ2dmO2aX;uJIF{e2G7 NVrsbVBROTBizszN MoroOFghcA>? NVrSNlJkUlOKLUKzJEgyOCEQvF2pJI9jfmmxdYPsfUBl\WO{ZXHz[YQhVkZvzsrCJGRPSSCkaX7kbY5oKGGldHn2bZR6 NXG5[WE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxNlU2PDhpPkOwNVI2PTR6PD;hQi=>
BV2 cells NIWwSXdHfW6ldHnvckBie3OjeR?= NEnidYE{OCEQvF2= NWPxS|hKOSCq Ml;UdJJmfHKnYYTt[Y51KG:oIFrTTE0zOyCmZXPy[YF{\WRidHjlJIxmfmWuczDv[kBKVC14IHHu[EBPVyCycn;keYN1cW:wIHnuJGxRWy2|dHnteYxifGWmIH3pZ5Jw\2yrYT6= MmXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6OUC0NVQoRjJ7OEmwOFE1RC:jPh?=
A549 cells M2XDUmNmdGxidnnhZoltcXS7IHHzd4F6 MlPGOUwhOTBuIEKwMEA{OCxiNECgZY5lKDVyIN88US=> MUGyOEBp NGTsc4N4cXSqIITo[UBqdmO{ZXHz[UBqdiCMU1itNlMh[2:wY3XueJJifGmxbjygeIhmKGmwaHnibZRqd25icnH0[UBnd3JiY3XscEB3cWGkaXzpeJkhf2G|IHfyZYR2[WyueTDpcoNz\WG|ZXSsJIFv\CC|aXfubYZq[2GwdDDkbYZn\XKnbnPld{BmgGm|dHXkJJdp\W5idHjlJGpUUC1{MzDjc45k\W62cnH0bY9vKHejczDndoVifGW{IIToZY4hOjBizszNMi=> Mk\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{OEG5OlEoRjJ6MkixPVYyRC:jPh?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
β1 Integrin / Fibronectin / p-Src / Src / α-SMA / NF-κB ; 

PubMed: 25170871     

Inhibition of constitutive activation of NF-κB inhibits the key signaling promoting proliferation/invasion and decreases the mesenchymal markers, Fibronectin and α-SMA. Cells were treated with indicated concentration of NF-κB activation Inhibitor II, JSH-䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞

IL-6 / IL-23; 

PubMed: 28821374     

Neutrophils were infected with MTB H37Rv alone or in the presence of the NF-κB-inhibitor JSH-23 (20 μM), PI3K-inhibitor LY294002 (30 μM), MEK1/2-inhibitor U0126 (40 μM), p38 inhibitor-SB203580 (10 μM) or JNK-inhibitor SP600125 (20 μM). The DMSO-treated ne䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ෆĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿

Draq5 / p65 ; 

PubMed: 31072360     

Immunofluorescence staining of p65 in CT26 cells after 24 h treatment with 10 μM JSH-23, 1 μg/ml LPS or JSH-23 + LPS together. Magnification: × 60.

In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]


Kinase Assay:[1]
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Measurement of NF-κB transcriptional activity:

Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
Cell Research:[1]
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  • Cell lines: RAW 264.7 cells
  • Concentrations: ~300 μM
  • Incubation Time: 24 hours
  • Method: Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: STZ-induced diabetic rats
  • Dosages: ~3 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 240.34


CAS No. 749886-87-1
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID