JSH-23

Catalog No.S7351

For research use only.

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.

JSH-23 Chemical Structure

CAS No. 749886-87-1

Selleck's JSH-23 has been cited by 116 publications

Purity & Quality Control

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Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
Targets
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro

JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HK-2 cells Mnz4SpVv[3Srb36gZZN{[Xl? NIjqUnAyODEkgJpOwG0> MV[z5qCKcA>? MUDwdoV1emWjdH3lcpQhf2m2aDDKV2guOjNiZX\m[YN1cX[nbImgZoxw[2unZDDBcochUUlvaX7keYNm\CCwdXPs[YFzKHB4NTDhZ4N2dXWuYYTpc44> M4jQW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOEe0OVQ1Lz5|MEi3OFU1PDxxYU6=
U2OS/DR-GFP cells NIPHT|VHfW6ldHnvckBie3OjeR?= M{fzdVExKGGwZDCyNEDDvU1? MknXTnNJNTJ|IITy[YF1dWWwdDDzbYdvcW[rY3HueIx6KGSnY4LlZZNm\CC2aHWgTHIh\nKncYXlcoN6 MkiwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB5N{C5NlQoRjNyN{ewPVI1RC:jPh?=
MCF-7:5C M3HUNWZ2dmO2aX;uJIF{e2G7 M4LoNVIxKM7:bX;sM2w> Mk\0N{BidmRiNjDkZZl{ Mn;KTnNJNTJ|IHPvcZBt\XSnbImgZoxw[2unZDDFNk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCPQ1[tO|o2SyClZXzsdy=> NI[4WIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKyOFQ{OCd-M{CyNlQ1OzB:L3G+
MCF-7:2A Ml2xSpVv[3Srb36gZZN{[Xl? NIDRSoUzOCEQvH3vcE9N MUKzJIFv\CB4IHThfZM> M32zWmpUUC1{MzDpcoNz\WG|ZXSgSVIucW6mdXPl[EBieG:ydH;zbZMhcW5iTVPGMVc7OkFiY3XscJM> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ{NESzNEc,OzB{MkS0N|A9N2F-
BMDM NFTVVGZHfW6ldHnvckBie3OjeR?= MVq2NEDPxE1? MlHTNVkuOjRiaB?= MnzVTnNJNTJ|IDi2NEDPxE1rIHnu[JVk\WRiYTDk[YNz\WG|ZTDv[kBKVC1zzsKgdoVt\WG|ZR?= NFrPbnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEGzPFMzOSd-M{CxN|g{OjF:L3G+
HL60 cells MXjGeY5kfGmxbjDhd5NigQ>? MmC2NVAh|ryP M{jme|Q5KGh? MYHKV2guOjNiKEGwJO69VSlib3L2bY92e2y7IHTlZ5Jm[XOnZDDOSk3PwkJiRF7BJIJqdmSrbnegZYN1cX[rdIm= NFzYcmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEGyOVU1QCd-M{CxNlU2PDh:L3G+
BV2 cells NI\SVVVHfW6ldHnvckBie3OjeR?= MknFN|Ah|ryP MlL2NUBp MofsdJJmfHKnYYTt[Y51KG:oIFrTTE0zOyCmZXPy[YF{\WRidHjlJIxmfmWuczDv[kBKVC14IHHu[EBPVyCycn;keYN1cW:wIHnuJGxRWy2|dHnteYxifGWmIH3pZ5Jw\2yrYT6= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh7MESxOEc,Ojl6OUC0NVQ9N2F-
A549 cells NES3O2dE\WyuII\pZYJqdGm2eTDhd5NigQ>? M{nad|UtKDFyLDCyNEwhOzBuIESwJIFv\CB3MDFOwG0> M1PxTVI1KGh? MXX3bZRpKHSqZTDpcoNz\WG|ZTDpckBLW0hvMkOgZ49v[2WwdILheIlwdixidHjlJIlvcGmkaYTpc44hemG2ZTDmc5Ih[2WubDD2bYFjcWyrdImge4F{KGe{YXT1ZYxtgSCrbnPy[YF{\WRuIHHu[EB{cWewaX\pZ4FvfCCmaX\m[ZJmdmOnczDlfIl{fGWmIIfo[Y4hfGinIFrTTE0zOyClb37j[Y51emG2aX;uJJdieyCpcnXheIVzKHSqYX6gNlAh|ryPLh?= NWLifIs2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyPFE6PjFpPkK4NlgyQTZzPD;hQi=>
Assay
Methods Test Index PMID
Western blot β1 Integrin / Fibronectin / p-Src / Src / α-SMA / NF-κB 25170871
ELSIA IL-6 / IL-23 28821374
Immunofluorescence Draq5 / p65 31072360
In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]

Protocol (from reference)

Kinase Assay:[1]
  • Measurement of NF-κB transcriptional activity:

    Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.

Cell Research:[1]
  • Cell lines: RAW 264.7 cells
  • Concentrations: ~300 μM
  • Incubation Time: 24 hours
  • Method: Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
Animal Research:[4]
  • Animal Models: STZ-induced diabetic rats
  • Dosages: ~3 mg/kg
  • Administration: Oral administration

Solubility (25°C)

In vitro

DMSO 48 mg/mL
(199.71 mM)
Ethanol 20 mg/mL
(83.21 mM)
Water Insoluble

Chemical Information

Molecular Weight 240.34
Formula

C16H20N2

CAS No. 749886-87-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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