For research use only.

Catalog No.S7351

105 publications

JSH-23 Chemical Structure

CAS No. 749886-87-1

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.

Selleck's JSH-23 has been cited by 105 publications

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro

JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HK-2 cells NFXmOoRHfW6ldHnvckBie3OjeR?= NXe2c|BvOTBy4pEJ{txO NYrzPINvO+LCiXi= NUXhNFdDeHKndILlZZRu\W62IIfpeIghUlOKLUKzJIVn\mWldHn2[Yx6KGKub3Pr[YQhSW6pIFnJMYlv\HWlZXSgcpVkdGWjcjDwOlUh[WOldX31cIF1cW:w M{GzflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOEe0OVQ1Lz5|MEi3OFU1PDxxYU6=
U2OS/DR-GFP cells M1HnTWZ2dmO2aX;uJIF{e2G7 M1q3VVExKGGwZDCyNEDDvU1? NWTMUWRLUlOKLUKzJJRz\WG2bXXueEB{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnZDD0bIUhUFJiZoLldZVmdmO7 M1jPUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyN{ewPVI1Lz5|MEe3NFkzPDxxYU6=
MCF-7:5C Ml\xSpVv[3Srb36gZZN{[Xl? MoD0NlAh|ryvb3yvUC=> NGfIepA{KGGwZDC2JIRigXN? Ml;CTnNJNTJ|IHPvcZBt\XSnbImgZoxw[2unZDDFNk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCPQ1[tO|o2SyClZXzsdy=> MlLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MkS0N|AoRjNyMkK0OFMxRC:jPh?=
MCF-7:2A NUXJc4R6TnWwY4Tpc44h[XO|YYm= M1PwSFIxKM7:bX;sM2w> NWD4SHhqOyCjbnSgOkBl[Xm| MmPLTnNJNTJ|IHnuZ5Jm[XOnZDDFNk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCPQ1[tO|ozSSClZXzsdy=> NF3Tfpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKyOFQ{OCd-M{CyNlQ1OzB:L3G+
BMDM MVjGeY5kfGmxbjDhd5NigQ>? NEPS[ow3OCEQvF2= NXT3c4lVOTlvMkSgbC=> M2TzVGpUUC1{MzCoOlAh|ryPKTDpcoR2[2WmIHGg[IVkemWjc3Wgc4YhUUxvMd8yJJJmdGWjc3W= M1PM[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUO4N|IyLz5|MEGzPFMzOTxxYU6=
HL60 cells NIrVdGZHfW6ldHnvckBie3OjeR?= Mn3INVAh|ryP NXPqNlVvPDhiaB?= M1T6Z2pUUC1{MzCoNVAh|ryPKTDvZpZqd3W|bImg[IVkemWjc3XkJG5HNc78QjDEUmEh[mmwZHnu[{Bi[3Srdnn0fS=> NXy5dXpIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxNlU2PDhpPkOwNVI2PTR6PD;hQi=>
BV2 cells NUTGUmdCTnWwY4Tpc44h[XO|YYm= NIL1cHc{OCEQvF2= MVyxJIg> NVvSfXFKeHKndILlZZRu\W62IH;mJGpUUC1{MzDk[YNz\WG|ZXSgeIhmKGyndnXsd{Bw\iCLTD22JIFv\CCQTzDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1q[3KxZ3zpZU4> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh7MESxOEc,Ojl6OUC0NVQ9N2F-
A549 cells NIrZco5E\WyuII\pZYJqdGm2eTDhd5NigQ>? NFrU[og2NCBzMDygNlAtKDNyLDC0NEBidmRiNUCg{txO M2\q[VI1KGh? NIK3eVl4cXSqIITo[UBqdmO{ZXHz[UBqdiCMU1itNlMh[2:wY3XueJJifGmxbjygeIhmKGmwaHnibZRqd25icnH0[UBnd3JiY3XscEB3cWGkaXzpeJkhf2G|IHfyZYR2[WyueTDpcoNz\WG|ZXSsJIFv\CC|aXfubYZq[2GwdDDkbYZn\XKnbnPld{BmgGm|dHXkJJdp\W5idHjlJGpUUC1{MzDjc45k\W62cnH0bY9vKHejczDndoVifGW{IIToZY4hOjBizszNMi=> MknZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{OEG5OlEoRjJ6MkixPVYyRC:jPh?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
β1 Integrin / Fibronectin / p-Src / Src / α-SMA / NF-κB ; 

PubMed: 25170871     

Inhibition of constitutive activation of NF-κB inhibits the key signaling promoting proliferation/invasion and decreases the mesenchymal markers, Fibronectin and α-SMA. Cells were treated with indicated concentration of NF-κB activation Inhibitor II, JSH-䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞

IL-6 / IL-23; 

PubMed: 28821374     

Neutrophils were infected with MTB H37Rv alone or in the presence of the NF-κB-inhibitor JSH-23 (20 μM), PI3K-inhibitor LY294002 (30 μM), MEK1/2-inhibitor U0126 (40 μM), p38 inhibitor-SB203580 (10 μM) or JNK-inhibitor SP600125 (20 μM). The DMSO-treated ne䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ෆĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿

Draq5 / p65 ; 

PubMed: 31072360     

Immunofluorescence staining of p65 in CT26 cells after 24 h treatment with 10 μM JSH-23, 1 μg/ml LPS or JSH-23 + LPS together. Magnification: × 60.

In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]


Kinase Assay:[1]
- Collapse

Measurement of NF-κB transcriptional activity:

Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
Cell Research:[1]
- Collapse
  • Cell lines: RAW 264.7 cells
  • Concentrations: ~300 μM
  • Incubation Time: 24 hours
  • Method: Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
    (Only for Reference)
Animal Research:[4]
- Collapse
  • Animal Models: STZ-induced diabetic rats
  • Dosages: ~3 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 240.34


CAS No. 749886-87-1
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

NF-κB Signaling Pathway Map

Related NF-κB Products

Tags: buy JSH-23|JSH-23 ic50|JSH-23 price|JSH-23 cost|JSH-23 solubility dmso|JSH-23 purchase|JSH-23 manufacturer|JSH-23 research buy|JSH-23 order|JSH-23 mouse|JSH-23 chemical structure|JSH-23 mw|JSH-23 molecular weight|JSH-23 datasheet|JSH-23 supplier|JSH-23 in vitro|JSH-23 cell line|JSH-23 concentration|JSH-23 nmr|JSH-23 in vivo|JSH-23 clinical trial|JSH-23 inhibitor|JSH-23 NF-κB inhibitor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID