Home Immunology & Inflammation ROS activator - TAK1 inhibitor - NF-κB inhibitor - IκB/IKK inhibitor - IRAK inhibitor Sarsasapogenin For research use only.

Sarsasapogenin

Synonyms: Parigenin

Sarsasapogenin (SAR, Parigenin) is a steroidal sapogenin. It can provoke the generation of reactive oxygen species and activate unfolded protein response (UPR) signaling pathways. SAR potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages.

Sarsasapogenin Chemical Structure

Sarsasapogenin Chemical Structure

CAS: 126-19-2

Selleck's Sarsasapogenin has been cited by 1 publication

Purity & Quality Control

Batch: S360701 Ethanol] 5 mg/mL] false] DMSO] Insoluble] false] Water] Insoluble] false Purity: 99.94%
99.94

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Biological Activity

Description Sarsasapogenin (SAR, Parigenin) is a steroidal sapogenin. It can provoke the generation of reactive oxygen species and activate unfolded protein response (UPR) signaling pathways. SAR potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages.
Targets
NF-κB [2] IRAK1 [2] TAK1 [2] IκBα [2]
In vitro
In vitro Sarsasapogenin induces an increase in the population of Hela cells in S phase and an obvious accumulation of cells in G2/M phase in a time-dependent manner. It induces apoptosis in HeLa cells via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin induces the activation of ER stress pathway. Sarsasapogenin induces cytotoxic effects in human cervical cancer cells via cell cycle arrest, ROS-mediated mitochondrial pathway and ER stress pathway. Sarsasapogenin induces the activation of UPR, the ER specific stress response at early stage and then activates CHOP, which may contribute to the initiation and augment of mitochondrial membrane permeabilization by dephosphorylation of Akt so as to mediate the apoptotic signals from ER to mitochondria[1]. Sarsasapogenin potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, as well as polarization of M2 to M1 macrophages[2].
Cell Research Cell lines Hela cells
Concentrations 0-60 μM
Incubation Time 48 h
Method

HeLa cells are incubated with sarsasapogenin (0-60 μM) for 48 h. Hoechst 33342-stained nuclei are visualized using confocal laser scanning microscopy.
In Vivo
In vivo Oral administration of sarsasapogenin inhibits 2,3,4-trinitrobenzene sulfonic acid (TNBS)-induced colon shortening and myeloperoxidase activity in mice, along with reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10. It inhibits Th17 cell differentiation in colonic lamina propria, but induces Treg cell differentiation. Sarsasapogenin potently inhibits inflammatory responses in vivo[2].
Animal Research Animal Models C57BL/6 mice
Dosages 5 or 10 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 416.64 Formula

C27H44O3
CAS No. 126-19-2 SDF Download Sarsasapogenin SDF
Smiles CC1CCC2(C(C3C(O2)CC4C3(CCC5C4CCC6C5(CCC(C6)O)C)C)C)OC1
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 5 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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