For research use only. Not for use in humans.

Denosumab (anti-RANK ligand)

Synonyms: AMG-162 Cat.No.A2020 Purity:99.68%

Denosumab (anti-RANK ligand) (AMG-162) is a fully human monoclonal antibody that selectively inhibits primate RANKL. Denosumab inhibits the ability of human RANKL (143–317) to stimulate the formation of osteoclasts derived from murine RAW 264.7 cells with an IC50 value of 1.64 nM.

Click to purchase the isotype control of Denosumab (anti-RANK ligand)

4 Citations

17 Nobel Prize winners have used Selleck products

Shimon Sakaguchi

Nature Communications 2025,16, Article number:1325

David Baker

Dev Cell 2023, 58(20):2163-2180.e9.

David Julius

Cell 2017, 185-198.e16

Michael Houghton

Cell Chem Biol, 2020, 27(7):780-792.e5

Charles M. Rice

Cell 2018, 172(3):423-438.e25

Quality Control

Batch: Purity: 99.68% Protein concentration: 5mg/ml Endotoxin Level: <1EU/mg

99.68

Specificity

Name	Citation	NF-κB	Others
QNZ (EVP4593)	155	++++	TNF-α
JSH-23	164	++
B022	0	++++
UCB-9260	0	+++	TNF
Morusin	0	+	STAT3
IQ 3	1	++	TNF-α,IL-6,JNK3
CBL0137	9	+++	FACT,p53
NIK SMI1	0	++++
Ginsenoside Rb3	1	++	iNOS,COX-2
Ginsenoside Rd	4	+	iNOS,COX-2,Ca2+ influx
CBL0137 HCl	9	+++	FACT,p53
SC75741	53	+++
TPCA-1	72	✔	STAT3,IKK2
Bardoxolone Methyl	35	✔	IKK,Ferroptosis,Nrf2
Curcumin	49	✔	Nrf2,Ferroptosis,HDAC
Sodium 4-Aminosalicylate	2	✔
Caffeic Acid Phenethyl Ester	55	✔
Sodium salicylate	1	✔
HOIPIN-8	0	✔
EN450	0	✔
Chitosan oligosaccharide	0	✔
NF-κB-IN-1	2	✔
SM-7368	0	✔
sappanone A	0	✔
Okanin	0	✔	TLR4
Rubiadin 1-methyl ether	0	✔
Cornuside	0	✔
BTYNB	0	✔
TCEP Hydrochloride	2	✔
IMM-H007	0	✔	ABCA1,JNK/AP1,AMPK
Urolithin B	1	✔	ERK,JNK,IκBα
L-Quebrachitol	0	✔	Wnt/β-catenin,MAPK,Runx2
Licochalcone D	0	✔	EGFR,JAK2,PARP
Tectochrysin	0	✔
Caulophylline (N-Methylcytisine)	0	✔	IκB,MPO,IKK
Myrislignan	1	✔	COX-2,iNOS,TNF-α
NDMC101	0	✔
Gardenoside	0	✔	IL-6,IL-1β,TNF-α
Cucurbitacin IIb	0	✔	IκBα,ERK1/2,JNK
Jaceosidin	0	✔	Mcl-1,Bax,COX-2
Isoliquiritin apioside	0	✔	MAPK,MMP
Withaferin A (WFA)	12	✔	vimentin
Rocaglamide	3	✔	eIF4A,HSF1
Xanthatin	0	✔	STATs
Alobresib (GS-5829)	0	✔	MYC,ERK1/2,Akt
Adjudin	1	✔	ERK,Cl⁻ channel
IAXO-102	0	✔	MAPK,TLR4
C25-140	0	✔	TRAF6-Ubc13
SN50	4	✔
INH14	0	✔	IKKβ,IKKα
TAK-243 (MLN7243)	72	✔	UAE
Sulforaphane	22	✔	Nrf2,HO-1,ROS
Madecassic acid	1	✔	IL-1β,TNF-α,COX-2
Homoplantaginin	1	✔	IL-6,IKKβ,TNF-α
(+)-Praeruptorin A	0	✔
2',5'-Dihydroxyacetophenone	1	✔	IL-6,TNF-α,ERK1/2
Dauricine	1	✔
8-O-acetyl shanzhiside methyl ester	0	✔
Forsythoside B	0	✔	IκB,IL-6,TNF-alpha
Engeletin	2	✔
Berbamine	8	✔	CaMKII,Bcr-Abl
Eleutheroside E	0	✔
Mulberroside A	0	✔	Caspase-1,NALP3,IL-6
Maslinic acid	1	✔
Chelidonic acid	0	✔	glutamate decarboxylase,IL-6,Caspase-1
Hyperoside	5	✔
Vanillic acid	0	✔
4'-Hydroxychalcone	0	✔
(+)-α-Lipoic acid	1	✔	LTR
4-Hydroxychalcone	2	✔
Neferine	5	✔
Benfotiamine	1	✔
Ginsenoside Rb1	4	✔	IFN-β,TNF-α,TLR4
Ginsenoside Rg1	5	✔	Aβ
Guaiacol	0	✔	COX-2
(E)-Cardamonin	0	✔	TRPA1
Hederagenin	0	✔	COX-2,iNOS
Ginsenoside Re	1	✔	JNK,Aβ
Scutellarin	1	✔	Akt,STAT3
Mangiferin	1	✔	Nrf2
Schisantherin A	0	✔
Curcumenol	3	✔	CYP3A4
Muscone	4	✔	TNF-α,IL-1β,NLRP3 inflammasome
Tyrosol	0	✔
Stachydrine	3	✔
Sodium Aescinate	2	✔
Dehydroevodiamine	0	✔	PGE2,iNOS,COX-2
Astragaloside IV	3	✔	mTOR,Akt,ERK
4'-Methoxyresveratrol	0	✔	NLRP3
Berbamine dihydrochloride	2	✔	CaMKII,Bcr-Abl
Sarsasapogenin	1	✔	IκBα,TAK1,IRAK1
(±)-Shikonin	38	✔	Proteasome,chemokine receptor,TNF-α
Diethylmaleate	0	✔	Nrf2
Omaveloxolone (RTA-408)	10	✔	Nrf2
DTP3	2	✔	GADD45β/MKK7
APX-3330	5	✔	APE1(Ref-1)
Triptolide	46	✔	MDM2,HSF1
Methylthiouracil	0	✔	ERK1/2,IL-6,TNF-α
Erdosteine	0	✔
Evodiamine	9	✔
Vanillylacetone	1	✔
(-)-Parthenolide	27	✔	p53,MDM2,HDAC1
Magnolol	7	✔
Indole-3-carbinol	3	✔
DHA (Dihydroartemisinin)	35	✔
Andrographolide	11	✔

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1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description	Denosumab (anti-RANK ligand) (AMG-162) is a fully human monoclonal antibody that selectively inhibits primate RANKL. Denosumab inhibits the ability of human RANKL (143–317) to stimulate the formation of osteoclasts derived from murine RAW 264.7 cells with an IC50 value of 1.64 nM.
References	https://pubmed.ncbi.nlm.nih.gov/19016581/ https://pubmed.ncbi.nlm.nih.gov/29435849/

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	615258-40-7
Molecular Weight	145.16 kDa
Isotype	human IgG1
Source	HEK293
Sterility	0.2 μM filtered
Formulation	PBS Buffer, pH 7.4
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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