Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

For research use only.

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate) is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Caffeic Acid Phenethyl Ester Chemical Structure

CAS No. 104594-70-9

Selleck's Caffeic Acid Phenethyl Ester has been cited by 40 publications

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Biological Activity

Description Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate) is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells M{fPUGZ2dmO2aX;uJIF{e2G7 NEL6NZIzPCCq M2PN[mlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNibnn0dol1\SCjY3P1cZVt[XSrb36gZYRucW6rc4TldoVlKDFiaIKgZoVnd3KnIFzQV{BkcGGubHXu[4Uh[W6mIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5MhemWjZ3XueEBie3OjeTygSWM2OD1yLkG5N{DPxE1? MnSyNVg3Pjd|MkC=
mouse 26-L5 cells MUHQdo9tcW[ncnH0bY9vKGG|c3H5 NYnoV5JDPzJiaB?= MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKDJ4LVy1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:OC5|IN88US=> MoTlNVIxOjd5M{m=
human HeLa cells MlvmVJJwdGmoZYLheIlwdiCjc4PhfS=> NHfOdWs4OiCq NVKzfoIxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;Mj6zOkDPxE1? MmnPNVIxOjd5M{m=
human Bewo cells NIjSeY5HfW6ldHnvckBie3OjeR?= NEWwPZJCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[ZdwKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIgh[nliTWTUJIF{e2G7LDDJR|UxRTJwOU[g{txO NHHiWGwzPTF4MEizOy=>
mouse J774.1 cells Mn;tSpVv[3Srb36gZZN{[Xl? MlPBNlQhcA>? MliyTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGmwIH3veZNmKEp5N{SuNUBk\WyuczDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO|YYmsJGlEPTB;ND64JO69VQ>? M3XHVFE2QTd2NkC4
human Bel7402 cells NF7yfndEgXSxdH;4bYPDqGG|c3H5 MlLoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRoVtPzRyMjDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;NT61JO69VQ>? MkS5NVg6PTJ2MkC=
human LNCAP cells NG\TdWNHfW6ldHnvckBie3OjeR?= NGPPcWQzPCCq NH3McXVCdnSrYX7kdo9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gSGhVNXO2aX31cIF1\WRiUGPBJJJmdGWjc3WgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVYvOiCwTR?= MUCyOFA5ODFyNR?=
mouse BV2 cells NIfGe|ZHfW6ldHnvckBie3OjeR?= NWDB[2ZnOjRiaB?= M2GwN2lvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgRnYzKGOnbHzzJJRz\WG2ZXSgN{BpenNiYnXmc5JmKEySUzDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPU[uOEDPxE1? MmjHNlM5PzB5MEC=
mouse B16-BL6 cells MUfQdo9tcW[ncnH0bY9vKGG|c3H5 M13Xd|czKGh? NIDXfVBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFKxOk1DVDZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME22Mlc6KM7:TR?= M3\oR|EzODJ5N{O5
HUVEC cells MmP4R5l1d3SxeHnjxsBie3OjeR?= NIrYeIIyQCCq Mmr1R5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCKMl:yMYlv\HWlZXSgc5hq\GG2aY\lJJN1emW|czDpckBJXV[HQzDj[YxteyCjZoTldkAyQCCqcoOgZpkhS2WubD3UbZRmeiCEbIXlJIF{e2G7 M2D4VlIxPTl6OEm0
human HT1080 cells M1jHPXBzd2yrZnXyZZRqd25iYYPzZZk> MkfBO|IhcA>? MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTlwNTFOwG0> NWDGPZBDOTJyMke3N|k>
human HL60 cells MYjDfZRwfG:6aXRCpIF{e2G7 NVTzS5p{PzJiaB?= Mli5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUmuPEDPxE1? NFPwcnozODZ5M{eyOy=>
human LS 174T cells Mom1R5l1d3SxeHnjxsBie3OjeR?= M{XpSVczKGh? NFnTbY9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNWyBzN{TUJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOlA:QS57IN88US=> M3zQUFIxPjd|N{K3
human SGC7901 cells NGq3N|ZHfW6ldHnvckBie3OjeR?= M4nsVmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPHR|c6ODFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDNWHQh[XO|YYmsJGlEPTB;MUSuNlYh|ryP MlfxNlUyPjB6M{e=
human Bel7404 cells Mn3YR5l1d3SxeHnjxsBie3OjeR?= MojrO|IhcA>? NH\sd3BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NEC0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTRwNTFOwG0> MorNNlA3PzN5Mke=
human BGC823 cells MoHnR5l1d3SxeHnjxsBie3OjeR?= Mn;4O|IhcA>? M{nNV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJISzh{MzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE6NjNizszN MmXFNlA3PzN5Mke=
human HO8910 cells NGnlSnZEgXSxdH;4bYPDqGG|c3H5 NF;XcZc4OiCq M1zEXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhQQDlzMDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI2NjVizszN NXPIVHNjOjB4N{O3Nlc>
human MCF7 cells NXvhT3drS3m2b4TvfIlkyqCjc4PhfS=> NX;lPVNVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;Mk[uO{DPxE1? MmW1NVg6PTJ2MkC=
human A549 cells NE\Yb4xEgXSxdH;4bYPDqGG|c3H5 M{jybVczKGh? NHvVb4REgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlYvQCEQvF2= MYKyNFY4Ozd{Nx?=
human LNCAP cells M3zMe2N6fG:2b4jpZ:Kh[XO|YYm= M1H2WVI1KGh? M{TpV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliV2PUMVEh[XO|YYmsJGlEPTB;M{OuO{DPxE1? NFHqT2YzPDB6MEGwOS=>
human HeLa cells MUfGeY5kfGmxbjDhd5NigQ>? MWTBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVM4Njl6IN88US=> NV6xeWpzOjVzNkC4N|c>
human HepG2 cells M{nWWGZ2dmO2aX;uJIF{e2G7 Mn3wRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwS|Ih[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCPVGSgZZN{[XluIFnDOVA:PDBwOEeg{txO MoTDNlUyPjB6M{e=
human ECA109 cells MoHIR5l1d3SxeHnjxsBie3OjeR?= NWD6WFF2PzJiaB?= NF7lOXREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGS0FzMEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Oi54IN88US=> NVm2OYd2OjB4N{O3Nlc>
human BCG823 cells M1fGZ2N6fG:2b4jpZ:Kh[XO|YYm= NETxO2VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDS0d6MkOgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUS0MlYh|ryP M3HlU|E5QTV{NEKw
human KB cells MnPPR5l1d3SxeHnjxsBie3OjeR?= MW[3NkBp MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTR3LkKg{txO Ml[2NlA3PzN5Mke=
human K562 cells MUPDfZRwfG:6aXRCpIF{e2G7 NXXhO2dYPzJiaB?= M17nSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20OkDPxE1? MV6yNFY4Ozd{Nx?=
rat VSMC M2XCW2Z2dmO2aX;uJIF{e2G7 NHPDUGc2KM7:TR?= NVLy[m93OzBibXnudy=> MVXE[YNz\WG|ZTDpckBqdnS{YXPlcIx2dGG{IGLPV{Bt\X[nbDDpckBRTEeILYP0bY12dGG2ZXSgdoF1KF[VTVOgZZQhPSC3TTDwdoV1emWjdHXkJJdqfGhiY3;tdI92dmRiZn;yJFMxKG2rboOgcYVie3W{ZXSgZYZ1\XJidYCgeI8hOjNiaILzJI9nKHCxc4Sgd5RqdXWuYYTpc44hfXOrbnegTFJFS0ZiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m| MWWyNVI3PTV3NB?=
rat PC12 cells MkHwSpVv[3Srb36gZZN{[Xl? MnqyNk42KM7:TR?= NXLMcGFoOyCq M3zYb25mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFiyU|IucW6mdXPl[EBl[W2jZ3WgbY4hemG2IGDDNVIh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZZQhOi53IIXNJJBz\WmwY4XiZZRm\CCob4KgN{BpenNiZn;scI94\WRiYomgTFJQOiClaHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhPSCqcoOgZpkhVVSWIHHzd4F6 MYqyOFY6PzN|NR?=
Assay
Methods Test Index PMID
Western blot p-p65 / CDH6 / RUNX2 / E-cadherin / N-cadherin / Vimentin / Slug / Snail ; Nrf2 ; AKT / AKT1 / AKT2 / AKT3 / p-AKT / FOXO1 / FOXO3a / p-FOXO1 / p-FOXO3a / Skp2 / p27(Kip) / Cyclin D1 / Rb / p-Rb 29284791 28275300 23615471
Immunofluorescence NF-κB 29284791
Growth inhibition assay Cell number 23615471
In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.

Animal Research:

[5]

  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Dosages: ~10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05157828 Not yet recruiting Other: No intervention Iron Deficiency Anemia of Pregnancy University of Cape Town February 2022 --
NCT04633434 Not yet recruiting Device: The Talk Parenting Skill for Alexa Parenting Practices|Self Efficacy|Stress|Child Behavior|Emotional Adjustment|Sleep Problems|Parent-Child Relations Oregon Research Behavioral Intervention Strategies Inc.|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) September 1 2021 Not Applicable
NCT05110417 Recruiting Drug: Pyridostigmine Bromide 60 Milligrams (mg) Dysphonia Spastic|Dysphonia|Laryngeal Dystonia Eastern Virginia Medical School May 20 2021 Phase 4
NCT04028674 Enrolling by invitation Device: Laryngeal Pacing Device Bilateral Vocal Fold Paralysis (BVFP) Vanderbilt University Medical Center|National Institute on Deafness and Other Communication Disorders (NIDCD) January 14 2021 Not Applicable
NCT04633473 Recruiting Behavioral: SIB-Time Web-Application Tool Family Relations|Sibling Relations Oregon Research Behavioral Intervention Strategies Inc. January 1 2021 Not Applicable
NCT03790956 Recruiting Procedure: Silk Microparticle Filler Injection Vocal Cord Paralysis Unilateral|Dysphonia|Dysphagia Oropharyngeal University of Southern California|Sofregen Medical Inc. July 1 2020 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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