Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Cited by 11 Publications

Carbohydr Polym, 2018, 202:134-142

PubMed: 30286986 ( click the link to review the publication )

Int J Mol Med, 2018, 41(1):555-563

PubMed: 29115406 ( click the link to review the publication )

Am J Physiol Cell Physiol, 2017, 313(6):C612-C620

PubMed: 29021196 ( click the link to review the publication )

Mol Med Rep, 2017, 16(4):4922-4926

PubMed: 28849197 ( click the link to review the publication )

Life Sci, 2017, 191:132-140

PubMed: 29080695 ( click the link to review the publication )

Int Immunopharmacol, 2017, 53:17-23

PubMed: 29031143 ( click the link to review the publication )

Mol Carcinog, 2017, 56(11):2434-2445

PubMed: 28618089 ( click the link to review the publication )

Tumour Biol, 2016, 37(9):12731-12742

PubMed: 27448305 ( click the link to review the publication )

Oncotarget, 2016, 7(25):38539-38550

PubMed: 27413117 ( click the link to review the publication )

Sci Rep, 2015, 5:12580

PubMed: 26219418 ( click the link to review the publication )

Oncotarget, 2014, 5(23):12189-202

PubMed: 25361008 ( click the link to review the publication )

5 Customer Reviews

The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.
HH1-1 promoted immune responses by activation of NF-κB signaling.

Carbohydr Polym, 2018, 202:134-142. Caffeic Acid Phenethyl Ester purchased from Selleck.

PC cells were treated with 5 μM CAPE for 24 h, and EMT markers were detected (n=3/group). * P < 0.05, ** P < 0.01, and *** P < 0.001

Oncotarget, 2016, 7(25):38539-38550. Caffeic Acid Phenethyl Ester purchased from Selleck.
Cells were treated with the NFκB inhibitor CAPE, which significantly decreased the CRH-induced IL-6 expression.

Mol Carcinog, 2017, 56(11):2434-2445. Caffeic Acid Phenethyl Ester purchased from Selleck.

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Targets

NF-κB ^[1]

In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. ^[1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. ^[2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. ^[3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
mouse RAW264.7 cells	MYnGeY5kfGmxbjDhd5NigQ>?		NWnmWmpbOjRiaB?=	NEe4eoZKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW\|I3PC55IHPlcIx{KGG\|c3Xzd4VlKGG\|IH7peJJqfGViYXPjeY12dGG2aX;uJIFldWmwaYP0[ZJm\CBzIHjyJIJm\m:{ZTDMVHMh[2ijbHzlcodmKGGwZDDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO\|YYmsJGVEPTB;MD6xPVMh\|ryP	Mn:yNVg3Pjd\|MkC=
mouse 26-L5 cells	Mn;yVJJwdGmoZYLheIlwdiCjc4PhfS=>		NXvCT\|NQPzJiaB?=	NGXIc\|JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIEK2MWw2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGVEPTB;MD6zJO69VQ>?	M2[0[\|EzODJ5N{O5
human HeLa cells	MorwVJJwdGmoZYLheIlwdiCjc4PhfS=>		NIf4bVg4OiCq	M4S0cmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR\|UxRTJwM{[g{txO	M4PhUFEzODJ5N{O5
human Bewo cells	M1G4dWZ2dmO2aX;uJIF{e2G7			NWS4SoVxSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRoV4dyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IF3UWEBie3OjeTygTWM2OD1{Lkm2JO69VQ>?	NID5VVMzPTF4MEizOy=>
mouse J774.1 cells	MljySpVv[3Srb36gZZN{[Xl?		M1vZfFI1KGh?	Mle3TY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGmwIH3veZNmKEp5N{SuNUBk\WyuczDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO\|YYmsJGlEPTB;ND64JO69VQ>?	NF3rWWsyPTl5NE[wPC=>
human Bel7402 cells	M1Hnd2N6fG:2b4jpZ:Kh[XO\|YYm=			NIjicFNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy\|IHL5JG1VXCCjc4PhfUwhUUN3ME21MlUh\|ryP	M1;PSVE5QTV{NEKw
human LNCAP cells	MXfGeY5kfGmxbjDhd5NigQ>?		MnTSNlQhcA>?	NX;kZ5NpSW62aXHu[JJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gUG5ESVBiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGRJXC2\|dHnteYxifGWmIGDTRUBz\WynYYPlJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF03NjJibl2=	MoHpNlQxQDBzMEW=
mouse BV2 cells	MknwSpVv[3Srb36gZZN{[Xl?		M2rqbVI1KGh?	MkXGTY5pcWKrdHnvckBw\iCwaYTybYMhd3irZHWgdJJw\HWldHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W\|ZTDCWlIh[2WubIOgeJJm[XSnZDCzJIhzeyCkZX\vdoUhVFCVIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5Mh[XO\|YYmsJGlEPTB;Nj60JO69VQ>?	M{P2Z\|I{QDdyN{Cw
mouse B16-BL6 cells	MUfQdo9tcW[ncnH0bY9vKGG\|c3H5		NX\QN2VzPzJiaB?=	NXPIZ\|A4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W\|ZTDCNVYuSkx4IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEWFNUC9Ok44QSEQvF2=	MkfGNVIxOjd5M{m=
HUVEC cells	MWPDfZRwfG:6aXRCpIF{e2G7		MmKwNVghcA>?	NHi3[4hEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFiyU\|IucW6mdXPl[EBwgGmmYYTpeoUhe3S{ZYPzJIlvKEiXVlXDJINmdGy\|IHHmeIVzKDF6IHjyd{BjgSCFZXzsMXRqfGW{IFLseYUh[XO\|YYm=	NH;ZeYQzODV7OEi5OC=>
human HT1080 cells	M2nCNHBzd2yrZnXyZZRqd25iYYPzZZk>		M3uzSlczKGh?	NFnhPFlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUNVA5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBGSzVyPUmuOUDPxE1?	NGf6XnAyOjB{N{ezPS=>
human HL60 cells	NWC1XI4zS3m2b4TvfIlkyqCjc4PhfS=>		M3;wR\|czKGh?	MmX4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUmuPEDPxE1?	NHnDVoIzODZ5M{eyOy=>
human LS 174T cells	MWTDfZRwfG:6aXRCpIF{e2G7		MX[3NkBp	MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMV{AyPzSWIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNkC9PU46KM7:TR?=	NEXMd2QzODZ5M{eyOy=>
human SGC7901 cells	MkTCSpVv[3Srb36gZZN{[Xl?			NILUO2FCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTS2M4QTBzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[2WubDDndo94fGhiYomgUXRVKGG\|c3H5MEBKSzVyPUG0MlI3KM7:TR?=	NF\0cWczPTF4MEizOy=>
human Bel7404 cells	NXXQNlNsS3m2b4TvfIlkyqCjc4PhfS=>		Mk[1O\|IhcA>?	M1HTS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJmdDd2MESgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPC53IN88US=>	M2r5XlIxPjd\|N{K3
human BGC823 cells	NI\TOGVEgXSxdH;4bYPDqGG\|c3H5		NEXT[oY4OiCq	NETOPYNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQS5\|IN88US=>	MX[yNFY4Ozd{Nx?=
human HO8910 cells	M1nlWmN6fG:2b4jpZ:Kh[XO\|YYm=		MnnOO\|IhcA>?	MnjZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTG85QTFyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlUvPSEQvF2=	MXyyNFY4Ozd{Nx?=
human MCF7 cells	Mnv3R5l1d3SxeHnjxsBie3OjeR?=			NHnCVmdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OD1{Nj63JO69VQ>?	NH21clEyQDl3MkSyNC=>
human A549 cells	M{S1[mN6fG:2b4jpZ:Kh[XO\|YYm=		MVK3NkBp	Mm\2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUK2Mlgh\|ryP	MX[yNFY4Ozd{Nx?=
human LNCAP cells	NUXEclBMS3m2b4TvfIlkyqCjc4PhfS=>		NFm3PWkzPCCq	M4HjUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliV2PUMVEh[XO\|YYmsJGlEPTB;M{OuO{DPxE1?	MXSyOFA5ODFyNR?=
human HeLa cells	MmHzSpVv[3Srb36gZZN{[Xl?			M13jO2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCPVGSgZZN{[XluIFnDOVA:OzdwOUig{txO	Mki0NlUyPjB6M{e=
human HepG2 cells	Mo\wSpVv[3Srb36gZZN{[Xl?			MmntRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwS\|Ih[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCPVGSgZZN{[XluIFnDOVA:PDBwOEeg{txO	MVWyOVE3ODh\|Nx?=
human ECA109 cells	MkOzR5l1d3SxeHnjxsBie3OjeR?=		NEKybmU4OiCq	NGTnU2NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGS0FzMEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Oi54IN88US=>	MXGyNFY4Ozd{Nx?=
human BCG823 cells	M2\hdWN6fG:2b4jpZ:Kh[XO\|YYm=			NH7sSHFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDS0d6MkOgZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyPUS0MlYh\|ryP	MXWxPFk2OjR{MB?=
human KB cells	M1fJR2N6fG:2b4jpZ:Kh[XO\|YYm=		MVq3NkBp	MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTR3LkKg{txO	MnLNNlA3PzN5Mke=
human K562 cells	MVvDfZRwfG:6aXRCpIF{e2G7		NXvtbWJrPzJiaB?=	NFK1OWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFYh\|ryP	NYO4cVVZOjB4N{O3Nlc>
rat VSMC	NGHVVZNHfW6ldHnvckBie3OjeR?=	M2O0dlUh\|ryP	NYnl[IdpOzBibXnudy=>	NYHJcmhnTGWlcnXhd4UhcW5iaX70doFk\WyudXzhdkBTV1NibHX2[YwhcW5iUFTHSk1{fGmvdXzheIVlKHKjdDDWV21EKGG2IEWgeW0heHKndILlZZRm\CC5aYToJINwdXCxdX7kJIZweiB\|MDDtbY5{KG2nYYP1doVlKGGodHXyJJVxKHSxIEKzJIhzeyCxZjDwc5N1KHO2aX31cIF1cW:wIIXzbY5oKEh{RFPGJIJ6KG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=>	NUm5fmlxOjF{NkW1OVQ>
rat PC12 cells	NGnNfHJHfW6ldHnvckBie3OjeR?=	NXjVVVZvOi53IN88US=>	NFj1Rpk{KGh?	M2TMOm5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFiyU\|IucW6mdXPl[EBl[W2jZ3WgbY4hemG2IGDDNVIh[2WubIOgZZN{\XO\|ZXSgZZMh[2WubDD2bYFjcWyrdImgZZQhOi53IIXNJJBz\WmwY4XiZZRm\CCob4KgN{BpenNiZn;scI94\WRiYomgTFJQOiClaHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhPSCqcoOgZpkhVVSWIHHzd4F6	NVPNTZFXOjR4OUezN\|U>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-p65 / CDH6 / RUNX2 / E-cadherin / N-cadherin / Vimentin / Slug / Snail ; PubMed: 29284791 Oncogenesis c/ Effect of Caffeic acid phenethyl ester on the expression of EMT markers, CDH6 and RUNX2 detected by western blotting analysis. e/ Effect of Caffeic acid phenethyl ester on the expression of EMT markers, CDH6 and RUNX2. Nrf2; PubMed: 28275300 World J Gastroenterol A: HSC-T6 cells were treated with 5 μmol/L, 10 μmol/L and 15 μmol/L CAPE for 24 h. Nrf2 mRNA and cytosol and nuclear protein expression levels were investigated using real-time PCR and Western blot, respectively AKT / AKT1 / AKT2 / AKT3 / p-AKT / FOXO1 / FOXO3a / p-FOXO1 / p-FOXO3a / Skp2 / p27(Kip) / Cyclin D1 / Rb / p-Rb ; PubMed: 23615471 Int J Mol Sci Effects of CAPE treatment on the abundance and phosphorylation status of signaling proteins. Protein expression of total Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr308, GSK3β, Rb, phospho-Rb Ser807/811, cyclin D1, and Skp2, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FOXO3a Thr32, NF-κB, phospho-NF-κB Ser536, p27(Kip), and β-actin in TW2.6 cells treated with 0, 50, or 100 μM CAPE for 48 h were assayed by Western blotting. Experiments were repeated three times.	29284791 28275300 23615471
Immunofluorescence	NF-κB; PubMed: 29284791 Oncogenesis NF-κB localization detected by IF assay in C666-1 cells when treated with Caffeic acid phenethyl ester. DMSO was the negative control. Green, p65; blue, H33342 staining (nuclei). In-NF-κB represents NF-κB inhibitor (the Caffeic acid phenethyl ester)	29284791
Growth inhibition assay	Cell number; PubMed: 23615471 Int J Mol Sci Effect of caffeic acid phenethyl ester (CAPE) on viability and proliferation of TW2.6 oral cancer cells. TW2.6 oral cancer cells were treated with increasing concentrations of CAPE for 24, 48, or 96 h and determined by Hoechst dye 33258-based 96-well proliferation assay (A) or by MTT (3,4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) 96-well assay (B), respectively, as described in Material and Methods. Cell numbers were normalized to control (DMSO treatment) of each treatment period. The mean and standard deviation represented the average and standard deviation respectively of the results from all 36 wells in the three experiments. Asterisk *** represents statistically significant difference p < 0.001 between the treated group and the control group.	23615471

In vivo

In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. ^[5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. ^[6]

Protocol

Cell Research: ^[2]	- Collapse Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines Concentrations: ~200 μM Incubation Time: 3 days Method: Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO₂. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells. (Only for Reference)
Animal Research: ^[5]	- Collapse Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells Formulation: PBS Dosages: ~10 mg/kg Administration: i.p. (Only for Reference)

Cell Research:

^[2]

- Collapse

Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
Concentrations: ~200 μM
Incubation Time: 3 days
Method:
Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO₂. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.

(Only for Reference)

Animal Research:

^[5]

- Collapse

Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
Formulation: PBS
Dosages: ~10 mg/kg
Administration: i.p.
(Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	57 mg/mL (200.48 mM)
	Ethanol	57 mg/mL (200.48 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Caffeic Acid Phenethyl Ester SDF

Molecular Weight	284.31
Formula	C₁₇H₁₆O₄
CAS No.	104594-70-9
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	CAPE, Phenylethyl Caffeate

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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-10-14)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04031872	Not yet recruiting	Drug: LY3200882	Colorectal Cancer Metastatic	The Netherlands Cancer Institute\|Vall d''Hebron Institute of Oncology\|Agendia\|European Organisation for Research and Treatment of Cancer - EORTC\|Azienda Ospedaliera Niguarda Cà Granda\|Fundación para la Investigación del Hospital Clínico de Valencia\|University of Campania Luigi Vanvitelli\|University of Turin Italy\|Eli Lilly and Company\|Catalan Institute of Health\|Universitaire Ziekenhuizen Leuven	February 2020	Phase 1\|Phase 2
NCT03749863	Not yet recruiting	Procedure: Serial PRP injections	Presbylarynx\|Atrophy; Larynx\|Dysphonia\|Vocal Cord Atrophy\|Presbylarynges	University of Southern California	November 1 2019	Not Applicable
NCT03790956	Not yet recruiting	Procedure: Silk Microparticle Filler Injection	Vocal Cord Paralysis Unilateral\|Dysphonia\|Dysphagia Oropharyngeal	University of Southern California\|Sofregen Medical Inc.	October 1 2019	Not Applicable
NCT04088955	Recruiting	Other: DigiMeds	Colon Cancer\|Breast Cancer\|Rectal Cancer	Proteus Digital Health Inc.	September 2019	--
NCT04028674	Not yet recruiting	Device: Laryngeal Pacing Device\|Drug: Botulinum toxin type A	Bilateral Vocal Fold Paralysis (BVFP)	Vanderbilt University Medical Center\|National Institute on Deafness and Other Communication Disorders (NIDCD)	September 2019	Early Phase 1

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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NF-κB Signaling Pathway Map

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Caffeic Acid Phenethyl Ester

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Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

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Molecular Weight Calculator

Total Molecular Weight: g/mol

Instructions to calculate molar mass (molecular weight) of a chemical compound:

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-10-14)

Tech Support

NF-κB Signaling Pathway Map

Related NF-κB Products

Choose Your Country or Region

By Signaling Pathways

By product type

Caffeic Acid Phenethyl Ester

Cited by 11 Publications

5 Customer Reviews

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Protocol

References

Solubility (25°C)

Chemical Information Download Caffeic Acid Phenethyl Ester SDF

Bio Calculators

Molarity Calculator

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

Dilution Calculator

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

The Serial Dilution Calculator Equation

Serial Dilutions

Computed Result

Molecular Weight Calculator

Total Molecular Weight: g/mol

Instructions to calculate molar mass (molecular weight) of a chemical compound:

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-10-14)

Tech Support

NF-κB Signaling Pathway Map

Related NF-κB Products

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)