Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Caffeic Acid Phenethyl Ester Chemical Structure

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Size Price Stock Quantity  
USD 97 In stock
USD 270 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 10 Publications

5 Customer Reviews

  • The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

    Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

    Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

    Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • HH1-1 promoted immune responses by activation of NF-κB signaling.

    Carbohydr Polym, 2018, 202:134-142. Caffeic Acid Phenethyl Ester purchased from Selleck.

    PC cells were treated with 5 μM CAPE for 24 h, and EMT markers were detected (n=3/group). * P < 0.05, ** P < 0.01, and *** P < 0.001

    Oncotarget, 2016, 7(25):38539-38550. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • Cells were treated with the NFκB inhibitor CAPE, which significantly decreased the CRH-induced IL-6 expression.

    Mol Carcinog, 2017, 56(11):2434-2445. Caffeic Acid Phenethyl Ester purchased from Selleck.

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NWL4e|JITnWwY4Tpc44h[XO|YYm= MmjHNlQhcA>? MojyTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCwaYTybZRmKGGlY4XteYxifGmxbjDh[I1qdmm|dHXy[YQhOSCqcjDi[YZwemViTGDTJINp[WyuZX7n[UBidmRibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bz\WGpZX70JIF{e2G7LDDFR|UxRTBwMUmzJO69VQ>? NIHNU24yQDZ4N{OyNC=>
mouse 26-L5 cells MmrzVJJwdGmoZYLheIlwdiCjc4PhfS=> NW\ZNI9PPzJiaB?= NUHwR5hVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTCyOk1NPSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBGSzVyPUCuN{DPxE1? MlvLNVIxOjd5M{m=
human HeLa cells NIP2dGtRem:uaX\ldoF1cW:wIHHzd4F6 MWC3NkBp Mke4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Oi5|NjFOwG0> NX2wcXFJOTJyMke3N|k>
human Bewo cells NYf2T40yTnWwY4Tpc44h[XO|YYm= NGDRfIxCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[ZdwKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIgh[nliTWTUJIF{e2G7LDDJR|UxRTJwOU[g{txO NF21[2gzPTF4MEizOy=>
mouse J774.1 cells MWrGeY5kfGmxbjDhd5NigQ>? NYPHTGFvOjRiaB?= M{m5ZmlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbjDtc5V{\SCMN{e0MlEh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHn[Y51KGG|c3H5MEBKSzVyPUSuPEDPxE1? MXOxOVk4PDZyOB?=
human Bel7402 cells M4W2eGN6fG:2b4jpZ:Kh[XO|YYm= M{W1eWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJmdDd2MEKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUWuOUDPxE1? M33kUFE5QTV{NEKw
human LNCAP cells NU\NVmpuTnWwY4Tpc44h[XO|YYm= NHLjVHQzPCCq M4HlfWFvfGmjbnTyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDETHQue3SrbYXsZZRm\CCSU1GgdoVt\WG|ZTDh[pRmeiB{NDDodpMh[nliRVzJV2EtKEmFNUC9Ok4zKG6P NFrRTWQzPDB6MEGwOS=>
mouse BV2 cells NVO1cWI{TnWwY4Tpc44h[XO|YYm= NHfzd24zPCCq MoHBTY5pcWKrdHnvckBw\iCwaYTybYMhd3irZHWgdJJw\HWldHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDCWlIh[2WubIOgeJJm[XSnZDCzJIhzeyCkZX\vdoUhVFCVIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5Mh[XO|YYmsJGlEPTB;Nj60JO69VQ>? M3K4V|I{QDdyN{Cw
mouse B16-BL6 cells NEW3SGJRem:uaX\ldoF1cW:wIHHzd4F6 MnW0O|IhcA>? MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEJzNj3CUFYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD14Lke5JO69VQ>? NX3pR4JuOTJyMke3N|k>
HUVEC cells MXrDfZRwfG:6aXRCpIF{e2G7 MkXVNVghcA>? MYDDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KEh{T{KtbY5lfWOnZDDvfIll[XSrdnWgd5Rz\XO|IHnuJGhWXkWFIHPlcIx{KGGodHXyJFE5KGi{czDifUBE\WyuLWTpeIVzKEKudXWgZZN{[Xl? MlfINlA2QTh6OUS=
human HT1080 cells NUjLSIRVWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVu3NkBp Mlj5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEGwPFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD17LkWg{txO MVSxNlAzPzd|OR?=
human HL60 cells NInVRWZEgXSxdH;4bYPDqGG|c3H5 MmHlO|IhcA>? NELwPY9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9PU45KM7:TR?= NVXPb4NCOjB4N{O3Nlc>
human LS 174T cells NEPwdYhEgXSxdH;4bYPDqGG|c3H5 MVe3NkBp NHTXS3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNWyBzN{TUJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOlA:QS57IN88US=> Ml\sNlA3PzN5Mke=
human SGC7901 cells MnruSpVv[3Srb36gZZN{[Xl? M3XmXGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPHR|c6ODFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDNWHQh[XO|YYmsJGlEPTB;MUSuNlYh|ryP NWrQbJpSOjVzNkC4N|c>
human Bel7404 cells MXXDfZRwfG:6aXRCpIF{e2G7 M3v4fVczKGh? NFfPOGVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NEC0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTRwNTFOwG0> MXOyNFY4Ozd{Nx?=
human BGC823 cells NFrUbWZEgXSxdH;4bYPDqGG|c3H5 MUS3NkBp NVe5[W1YS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkeFOEKzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTlwMzFOwG0> MV[yNFY4Ozd{Nx?=
human HO8910 cells NGrofVREgXSxdH;4bYPDqGG|c3H5 MXW3NkBp NVXBeG5[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUE96OUGwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OjVwNTFOwG0> NX7qfnBQOjB4N{O3Nlc>
human MCF7 cells MmLwR5l1d3SxeHnjxsBie3OjeR?= MmPNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OjZwNzFOwG0> MnLJNVg6PTJ2MkC=
human A549 cells MYXDfZRwfG:6aXRCpIF{e2G7 M2PRPFczKGh? NHjU[nBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlYvQCEQvF2= NXzsXXA2OjB4N{O3Nlc>
human LNCAP cells Mn7CR5l1d3SxeHnjxsBie3OjeR?= NF\QUYozPCCq MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOjRiaILzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxRTN|Lkeg{txO MVGyOFA5ODFyNR?=
human HeLa cells MlX4SpVv[3Srb36gZZN{[Xl? NVXvfFJOSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IF3UWEBie3OjeTygTWM2OD1|Nz65PEDPxE1? NEjUXmMzPTF4MEizOy=>
human HepG2 cells MlrjSpVv[3Srb36gZZN{[Xl? M2XJUWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIgh[nliTWTUJIF{e2G7LDDJR|UxRTRyLki3JO69VQ>? MlH2NlUyPjB6M{e=
human ECA109 cells NFf1d5VEgXSxdH;4bYPDqGG|c3H5 MorzO|IhcA>? NFTUNmREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGS0FzMEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Oi54IN88US=> MWGyNFY4Ozd{Nx?=
human BCG823 cells NGLQTGJEgXSxdH;4bYPDqGG|c3H5 NYXiTpV1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkOJOEKzJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME20OE43KM7:TR?= M4jB[|E5QTV{NEKw
human KB cells MVLDfZRwfG:6aXRCpIF{e2G7 NWe5dm42PzJiaB?= NY\6dnRES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20OU4zKM7:TR?= NGT2XXkzODZ5M{eyOy=>
human K562 cells MV;DfZRwfG:6aXRCpIF{e2G7 M2DoOlczKGh? M3TvfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20OkDPxE1? MUOyNFY4Ozd{Nx?=
rat VSMC NWrk[ndWTnWwY4Tpc44h[XO|YYm= Ml;VOUDPxE1? Mkf1N|AhdWmwcx?= MlSzSIVkemWjc3WgbY4hcW62cnHj[YxtfWyjcjDSU3MhdGW4ZXygbY4hWESJRj3zeIlufWyjdHXkJJJifCCYU13DJIF1KDVidV2gdJJmfHKnYYTl[EB4cXSqIHPvcZBwfW6mIH\vdkA{OCCvaX7zJI1m[XO3cnXkJIFnfGW{IIXwJJRwKDJ|IHjyd{Bw\iCyb4P0JJN1cW23bHH0bY9vKHW|aX7nJGgzTEOIIHL5JIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= NWHmV4Q2OjF{NkW1OVQ>
rat PC12 cells MUjGeY5kfGmxbjDhd5NigQ>? Mm\ONk42KM7:TR?= MmrXN{Bp MYnO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCKMl:yMYlv\HWlZXSg[IFu[WenIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHH0JFIvPSC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFUhcHK|IHL5JG1VXCCjc4PhfS=> M2K4TFI1Pjl5M{O1

... Click to View More Cell Line Experimental Data

In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Formulation: PBS
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (200.48 mM)
Ethanol 57 mg/mL (200.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage powder
in solvent
Synonyms CAPE, Phenylethyl Caffeate

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04031872 Not yet recruiting Drug: LY3200882 Colorectal Cancer Metastatic The Netherlands Cancer Institute|Vall d''Hebron Institute of Oncology|Agendia|European Organisation for Research and Treatment of Cancer - EORTC|Azienda Ospedaliera Niguarda Cà Granda|Fundación para la Investigación del Hospital Clínico de Valencia|University of Campania Luigi Vanvitelli|University of Turin Italy|Eli Lilly and Company|Catalan Institute of Health|Universitaire Ziekenhuizen Leuven February 2020 Phase 1|Phase 2
NCT03749863 Not yet recruiting Procedure: Serial PRP injections Presbylarynx|Atrophy; Larynx|Dysphonia|Vocal Cord Atrophy|Presbylarynges University of Southern California November 1 2019 Not Applicable
NCT03790956 Not yet recruiting Procedure: Silk Microparticle Filler Injection Vocal Cord Paralysis Unilateral|Dysphonia|Dysphagia Oropharyngeal University of Southern California|Sofregen Medical Inc. October 1 2019 Not Applicable
NCT04088955 Recruiting Other: DigiMeds Colon Cancer|Breast Cancer|Rectal Cancer Proteus Digital Health Inc. September 2019 --
NCT04028674 Not yet recruiting Device: Laryngeal Pacing Device|Drug: Botulinum toxin type A Bilateral Vocal Fold Paralysis (BVFP) Vanderbilt University Medical Center|National Institute on Deafness and Other Communication Disorders (NIDCD) September 2019 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

NF-κB Signaling Pathway Map

Related NF-κB Products

Tags: buy Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester supplier | purchase Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester cost | Caffeic Acid Phenethyl Ester manufacturer | order Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID