Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Caffeic Acid Phenethyl Ester Chemical Structure

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

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Cited by 8 Publications

5 Customer Reviews

  • The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

    Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

    Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

    Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • HH1-1 promoted immune responses by activation of NF-κB signaling.

    Carbohydr Polym, 2018, 202:134-142. Caffeic Acid Phenethyl Ester purchased from Selleck.

    PC cells were treated with 5 μM CAPE for 24 h, and EMT markers were detected (n=3/group). * P < 0.05, ** P < 0.01, and *** P < 0.001

    Oncotarget, 2016, 7(25):38539-38550. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • Cells were treated with the NFκB inhibitor CAPE, which significantly decreased the CRH-induced IL-6 expression.

    Mol Carcinog, 2017, 56(11):2434-2445. Caffeic Acid Phenethyl Ester purchased from Selleck.

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Biological Activity

Description Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NXH2cmpmTnWwY4Tpc44h[XO|YYm= M3TzRlI1KGh? NEHNNYNKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IH7peJJqfGViYXPjeY12dGG2aX;uJIFldWmwaYP0[ZJm\CBzIHjyJIJm\m:{ZTDMVHMh[2ijbHzlcodmKGGwZDDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO|YYmsJGVEPTB;MD6xPVMh|ryP NXXCT5g2OTh4NkezNlA>
mouse 26-L5 cells MnnrVJJwdGmoZYLheIlwdiCjc4PhfS=> MnvzO|IhcA>? M3PxeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViMk[tUFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1yLkOg{txO MnK3NVIxOjd5M{m=
human HeLa cells NXrLNXViWHKxbHnm[ZJifGmxbjDhd5NigQ>? NFLmeFg4OiCq MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiRVO1NF0zNjN4IN88US=> NEPCS3UyOjB{N{ezPS=>
human Bewo cells MUfGeY5kfGmxbjDhd5NigQ>? MUjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEZYfvJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQTZizszN Mmf2NlUyPjB6M{e=
mouse J774.1 cells NVfnVW53TnWwY4Tpc44h[XO|YYm= MX2yOEBp NUnhflR5UW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBPVyCycn;keYN1cW:wIHnuJI1wfXOnIFq3O|QvOSClZXzsd{Bi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYdmdnRiYYPzZZktKEmFNUC9OE45KM7:TR?= M2e5ZlE2QTd2NkC4
human Bel7402 cells MnrOR5l1d3SxeHnjxsBie3OjeR?= M1jBZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJmdDd2MEKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUWuOUDPxE1? NHPXXXkyQDl3MkSyNC=>
human LNCAP cells NUnWUW1ITnWwY4Tpc44h[XO|YYm= NEXsOpozPCCq M2TIPWFvfGmjbnTyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDETHQue3SrbYXsZZRm\CCSU1GgdoVt\WG|ZTDh[pRmeiB{NDDodpMh[nliRVzJV2EtKEmFNUC9Ok4zKG6P M13HPVI1ODhyMUC1
mouse BV2 cells MojtSpVv[3Srb36gZZN{[Xl? MoryNlQhcA>? NYnlZYJPUW6qaXLpeIlwdiCxZjDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBDXjJiY3XscJMhfHKnYYTl[EA{KGi{czDi[YZwemViTGDTJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDHdolme3NiYYPzZZktKEmFNUC9Ok41KM7:TR?= NVfNbmRGOjN6N{C3NFA>
mouse B16-BL6 cells MlSwVJJwdGmoZYLheIlwdiCjc4PhfS=> MlG4O|IhcA>? NHP2WmtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFKxOk1DVDZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME22Mlc6KM7:TR?= MmDJNVIxOjd5M{m=
HUVEC cells NVnYOpprS3m2b4TvfIlkyqCjc4PhfS=> M3XuSFE5KGh? M4D4RWN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiSELPNk1qdmS3Y3XkJI95cWSjdHn2[UB{fHKnc4OgbY4hUFWYRVOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KEOnbHytWIl1\XJiQnz1[UBie3OjeR?= M1qz[FIxPTl6OEm0
human HT1080 cells NIrjNZFRem:uaX\ldoF1cW:wIHHzd4F6 MYS3NkBp MlT0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEGwPFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD17LkWg{txO NFe0W4QyOjB{N{ezPS=>
human HL60 cells Mo\aR5l1d3SxeHnjxsBie3OjeR?= MmPyO|IhcA>? NWHvbJJES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVkvQCEQvF2= MkC4NlA3PzN5Mke=
human LS 174T cells NW\wbpZVS3m2b4TvfIlkyqCjc4PhfS=> MoflO|IhcA>? M2\KUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxUKDF5NGSgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO2NF06NjlizszN MoPqNlA3PzN5Mke=
human SGC7901 cells MWnGeY5kfGmxbjDhd5NigQ>? NYTyeIlXSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gV2dEPzlyMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHL5JG1VXCCjc4PhfUwhUUN3ME2xOE4zPiEQvF2= NImxNHMzPTF4MEizOy=>
human Bel7404 cells NGWyXm9EgXSxdH;4bYPDqGG|c3H5 NG\TeI44OiCq MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB2IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVQvPSEQvF2= MXuyNFY4Ozd{Nx?=
human BGC823 cells NEXkXpNEgXSxdH;4bYPDqGG|c3H5 NWrDcXViPzJiaB?= MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPU4{KM7:TR?= NFjQe2kzODZ5M{eyOy=>
human HO8910 cells NXnXO3NIS3m2b4TvfIlkyqCjc4PhfS=> NXvyTIlGPzJiaB?= NW\rUmRkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUE96OUGwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OjVwNTFOwG0> NInrXHAzODZ5M{eyOy=>
human MCF7 cells NWPZWlRPS3m2b4TvfIlkyqCjc4PhfS=> MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0zPi55IN88US=> Ml;wNVg6PTJ2MkC=
human A549 cells NVv5PXpLS3m2b4TvfIlkyqCjc4PhfS=> NEfDT3k4OiCq MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mk[uPEDPxE1? NH7HO2czODZ5M{eyOy=>
human LNCAP cells NYTTSpNNS3m2b4TvfIlkyqCjc4PhfS=> NVi5bnFCOjRiaB?= MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOjRiaILzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxRTN|Lkeg{txO NUG5RXJMOjRyOECxNFU>
human HeLa cells NFSy[4hHfW6ldHnvckBie3OjeR?= MWDBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVM4Njl6IN88US=> NFnUXnozPTF4MEizOy=>
human HepG2 cells MmDaSpVv[3Srb36gZZN{[Xl? NWD5VGpGSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDNWHQh[XO|YYmsJGlEPTB;NECuPFch|ryP NFfJZ2IzPTF4MEizOy=>
human ECA109 cells NUDaR|BGS3m2b4TvfIlkyqCjc4PhfS=> M2r5TlczKGh? MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFR2EyODliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20Nk43KM7:TR?= M4m0c|IxPjd|N{K3
human BCG823 cells M371UWN6fG:2b4jpZ:Kh[XO|YYm= MoT4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmNIQDJ|IHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OD12ND62JO69VQ>? NVjvO3NwOTh7NUK0NlA>
human KB cells M3Txb2N6fG:2b4jpZ:Kh[XO|YYm= M2TPOlczKGh? MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTR3LkKg{txO NYnmfHB6OjB4N{O3Nlc>
human K562 cells NXvUR25yS3m2b4TvfIlkyqCjc4PhfS=> MoDIO|IhcA>? Mnm4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUS2JO69VQ>? MY[yNFY4Ozd{Nx?=
rat VSMC NGHNZYlHfW6ldHnvckBie3OjeR?= NVjzSHB6PSEQvF2= NIHOeoY{OCCvaX7z NV[wbZF1TGWlcnXhd4UhcW5iaX70doFk\WyudXzhdkBTV1NibHX2[YwhcW5iUFTHSk1{fGmvdXzheIVlKHKjdDDWV21EKGG2IEWgeW0heHKndILlZZRm\CC5aYToJINwdXCxdX7kJIZweiB|MDDtbY5{KG2nYYP1doVlKGGodHXyJJVxKHSxIEKzJIhzeyCxZjDwc5N1KHO2aX31cIF1cW:wIIXzbY5oKEh{RFPGJIJ6KG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> M2W1UFIyOjZ3NUW0
rat PC12 cells NGnVZZVHfW6ldHnvckBie3OjeR?= NW\pbYU2Oi53IN88US=> M1P3ZlMhcA>? MUPO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCKMl:yMYlv\HWlZXSg[IFu[WenIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHH0JFIvPSC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFUhcHK|IHL5JG1VXCCjc4PhfS=> NWjuXHhOOjR4OUezN|U>

... Click to View More Cell Line Experimental Data

In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Formulation: PBS
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (200.48 mM)
Ethanol 57 mg/mL (200.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage powder
in solvent
Synonyms CAPE, Phenylethyl Caffeate

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03818685 Not yet recruiting Breast Cancer|Triple Negative Breast Neoplasms Centre Leon Berard April 1 2019 Phase 2
NCT03818685 Not yet recruiting Breast Cancer|Triple Negative Breast Neoplasms Centre Leon Berard April 1 2019 Phase 2
NCT03869892 Not yet recruiting Metastatic Colorectal Cancer Institut de Recherches Internationales Servier|ADIR a Servier Group company|Servier March 20 2019 Phase 3
NCT03873532 Not yet recruiting Biliary Tract Cancer Hutchison Medipharma Limited March 2019 Phase 2|Phase 3
NCT03579758 Not yet recruiting Stage I Gallbladder Cancer AJCC v8|Stage II Gallbladder Cancer AJCC v8|Stage IIA Gallbladder Cancer AJCC v8|Stage IIB Gallbladder Cancer AJCC v8|Stage III Gallbladder Cancer AJCC v8|Stage IIIA Gallbladder Cancer AJCC v8|Stage IIIB Gallbladder Cancer AJCC v8 Emory University March 15 2019 Phase 3
NCT03813784 Not yet recruiting Gastric Cancer|GastroEsophageal Cancer Jiangsu HengRui Medicine Co. Ltd. March 2019 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID