Home MAPK ERK inhibitor - NF-κB inhibitor Hypaphorine For research use only.

Hypaphorine

Hypaphorine which is abundantly found in vaccaria semen, counteracts inflammation via inhibition of ERK or/and NFκB signaling pathways.

Hypaphorine Chemical Structure

Hypaphorine Chemical Structure

CAS: 487-58-1

Purity & Quality Control

Batch: S091101 Water] 16 mg/mL] false] DMSO] Insoluble] false] Ethanol] Insoluble] false Purity: 99.99%
99.99

Hypaphorine Related Products

Signaling Pathway

ERK Signaling Pathways

ERK Signaling Pathway

Choose Selective ERK Inhibitors

Biological Activity

Description Hypaphorine which is abundantly found in vaccaria semen, counteracts inflammation via inhibition of ERK or/and NFκB signaling pathways.
In vitro
In vitro

Hypaphorine down-regulates LPS-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), retards LPS-induced phosphorylation of ERK, nuclear factor kappa beta (NFκB), NFκB inhibitor IκBα, and IKKβ, also eliminates the nuclear translocation of NFκB in LPS-treated RAW 264.7 cells.[1]
Cell Research Cell lines RAW 264.7 cells
Concentrations 6.25, 12.5, 25, 50, 100, 200 μM
Incubation Time 24 h
Method

The cell growth was arrested by incubation of the cells in 2% serum medium for 24 h before treatment. The indicated cells were then seeded in 96-well culture plates at a density of 5×10<sup>3</sup> cells/well, and stimulated with different concentrations of vaccaria hypaphorine (6.25, 12.5, 25, 50, 100, 200 μM) at 37℃ in 5% CO<sub>2</sub> saturated humidity condition for 24 h. Finally, the optical density (OD) was measured at 540 nm.
In Vivo
In vivo

Hypaphorine impairs RANKL-induced osteoclastogenesis through reduction of extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinase (JNK) and NF-κB p65 phosphorylation, which is considered as potential therapeutic agent for treating osteoclast-based bone loss.[2]
Animal Research Animal Models C57BL/6J mice induced by LPS
Dosages 10 and 30 mg/kg
Administration p.o.

Chemical Information & Solubility

Molecular Weight 246.3 Formula

C14H18N2O2
CAS No. 487-58-1 SDF --
Smiles C[N+](C)(C)C(CC1=CNC2=CC=CC=C21)C(=O)[O-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 16 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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