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Hederagenin NOS inhibitor

Name: Hederagenin
Brand: Selleck Chemicals
SKU: S3899
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S3899

Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. This compound inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.

Chemical Structure

Molecular Weight: 472.70

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Quality Control

Batch: S389901 DMSO]94 mg/mL]false]]]false]]]false Purity: 98%

Cited in Nature Medicine for its top-tier quality
COA
SDS
Datasheet

Related Targets	PD-1/PD-L1 CXCR STING AhR Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX
Other NOS Inhibitors	L-NAME HCl 1400W 2HCl TPEN (3R,8S)-Falcarindiol L-NMMA acetate 2',5'-Dihydroxyacetophenone ZLc002 6-Biopterin Prim-o-glucosylcimifugin L-Canavanine sulfate

Solubility

In vitro
Batch:

DMSO : 94 mg/mL (198.85 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.800mg/ml (1.69mM)	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	472.70	Formula	C₃₀H₄₈O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	465-99-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E			Smiles	CC1(CCC2(CCC3(C(=CCC4C3(CCC5C4(CCC(C5(C)CO)O)C)C)C2C1)C)C(=O)O)C

Mechanism of Action

Targets/IC₅₀/Ki	iNOS COX-2 NF-κB
In vitro	Hederagenin could induce apoptosis of LoVo cells through the mitochondrial apoptotic pathway. This compound could induce the up-regulation of Bax and down-regulation of Bcl-2, Bcl-xL and Survivin, the release of cytochrome c, followed by procaspase-9, procaspase-3 and proPARP cleavage. It exhibits antiinflammatory activities via decreasing production of pro-inflammatory mediators (NO, PGE2, TNF-α, IL-1β, and IL-6) through suppression of the pathways of NF-κB in LPS-induced RAW264.7 cells.
References	[1] https://pubmed.ncbi.nlm.nih.gov/25342273/ [2] https://pubmed.ncbi.nlm.nih.gov/26481049/

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