QNZ (EVP4593)

Catalog No.S4902

For research use only.

QNZ (EVP4593) shows potent inhibitory activity toward both NF-κB activation and TNF-α production with IC50 of 11 nM and 7 nM in Jurkat T cells, respectively.

QNZ (EVP4593) Chemical Structure

CAS No. 545380-34-5

Selleck's QNZ (EVP4593) has been cited by 98 publications

Purity & Quality Control

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Biological Activity

Description QNZ (EVP4593) shows potent inhibitory activity toward both NF-κB activation and TNF-α production with IC50 of 11 nM and 7 nM in Jurkat T cells, respectively.
Targets
TNF-α [1]
(Jurkat T cells)
NF-κB [1]
(Jurkat T cells)
7 nM 11 nM
In vitro

EVP4593 inhibits TNF-a production from murine splenocytes stimulated with LPS with IC50 of 7 nM. [1] EVP4593 (300 nM) entails a significant decrease of amplitude of store-operated currents (approximately by 60%), induced by application of 1 μM thapsigargin in Htt138Q cells, thus prevents abnormal store-operated calcium entry. EVP4593 is able to inhibit the activity of channels containing TRPC1 as one of the subunits, but has no effect on homooligomer channels composed exclusively of TRPC1. [2] QNZ (40 nM) completely abolishes the enhancement of neurite number and length evoked by laminin treatment of the schwann cells. QNZ reduces the neurite length by 61.36% of the schwann cells. QNZ significantly inhibits laminin-induced neurite outgrowth. QNZ also greatly diminishes the neurite elongation after 72 hours culture implying that both initial sprouting and longer term growth and extension seen in response to schwann cells seeded on laminin is mediated by NF-κB. [3] QNZ (10 nM) abolishes LPS-induced up-regulation of CSE expression in rat neutrophil. [4] QNZ (100 nM) blocks the induction effects of GRO/KC on K currents in IB4-negative neurons. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jurkat MVjLbY5ie2ViYYPzZZk> MlnlglExKM7:TR?= MnrvZ4F2e2W|IF7GMe67SiCLbnjpZol1cW:wIIfpeIghTUN3MDDv[kA6KG6P M1HTflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{CwNlE{Lz5{MUewNFIyOzxxYU6=
Jurkat M3PsO2Z2dmO2aX;uJIF{e2G7 NX\hXHNTOyEQvF2= MoLrZ4F2e2W|IGPPR{BKdmirYnn0bY9v MoX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MECyNVMoRjJzN{CwNlE{RC:jPh?=
neurons M1qwXmZ2dmO2aX;uJIF{e2G7 M3z6UVMxOCCwTR?= NY\JdXZmcW6qaXLpeEB{fG:{ZT3vdIVz[XSnZDDDZVIsKGWwdIL5 M3nIUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{CwNlE{Lz5{MUewNFIyOzxxYU6=
SK-N-SH MYnGeY5kfGmxbjDhd5NigQ>? M{faUVMxOCCwTR?= NEnHeJZqdmirYnn0d{BVWlCFMT3TeZBxd3K2ZXSgV29EKEOjMjugZ5VzemWwdIO= MmPvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MECyNVMoRjJzN{CwNlE{RC:jPh?=
neurons MVjGeY5kfGmxbjDhd5NigQ>? NIrNPIo{ODBibl2= M1HuOJBzd3SnY4SgXWFEOTJ6IF3TUkBnem:vIHfseZRidWG2ZTD0c5hq[2m2eR?= MljFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MECyNVMoRjJzN{CwNlE{RC:jPh?=
GABA MS-like neurons NFTXUnFHfW6ldHnvckBie3OjeR?= NWLZN29lOTByIH7N NFv4[INz\XOldXXzJIFjdm:{bXHsJHNQSy2vZXTpZZRm\CClYXzjbZVuKGWwdIL5 MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB6MEGyPUc,OjdyOECxNlk9N2F-
GABA MS-like neurons MnzGSpVv[3Srb36gZZN{[Xl? NH;kOZgyODByIH7N M2f3[Y5wem2jbHn6[ZMhfGinIH71cYJmeiCxZjDsfZNwe2:vZYOvZZV1d3CqYXfvd49u\XN? NIDPXmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4NFEzQSd-MkewPFAyOjl:L3G+
GABA MS-like neurons NELFcXVHfW6ldHnvckBie3OjeR?= M3\McVExOCCwTR?= MXPy[ZNkfWW|IHHnbY5oKG6ndYLvcpMh\nKxbTDj[YxtKGSnYYTo Mn7GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOECxNlkoRjJ5MEiwNVI6RC:jPh?=
HepG2 NY\YPXZZTnWwY4Tpc44h[XO|YYm= NHfCenBJSVKYQWLEPkBKdmirYnn0bY9vKG:oIHzpeoVzKHO2YXflJHBt[XOvb3TpeY0h[mW{Z3jlbUBqdm[nY4Tpc44hcW5iSHXwS|Ih[2WubIOsJGlEPTBiPTCwMlAyOiEQvF2u MkK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OE[xNlQoRjJ{NUi2NVI1RC:jPh?=
HepG2 NX7qWJNoTnWwY4Tpc44h[XO|YYm= M37wb|AvOSC2bzCxNEB2VQ>? MXWxJIhz NV3qN3hpUW6qaXLpeIlwdiCxZjDUUmZidHCqYT3pcoR2[2WmIF7Gb4FxeGGEIDj1cotvd3ewIH;ybYdqdilvZHXw[Y5l\W62IITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZZQhOC5zIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOSCqcjDwdolweiC2bzDUUmZidHCqYTDpcoR2[3Srb36gcYVie3W{ZXSgZYZ1\XJiNjDodpMh[nliZIXhcE1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? NHvaTWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KxPVg2PCd-MkOyNVk5PTR:L3G+
Assay
Methods Test Index PMID
Western blot NF-κB p65 / OPN / Tissue factor / Thrombin ; VEGF / TNF-α / IL-1β / IL-6 / MMP-2 / MMP-9 / NF-κB p65(Ser536) 29061995 28693192
Growth inhibition assay Cell viability 28693192
In vivo EVP4593 (1 mg/kg, i.p.) dose-dependently inhibits carrageenin-induced paw edema in rats. [1]

Protocol (from reference)

Animal Research:[1]
  • Animal Models: male SD rats with carrageenin induced paw edema
  • Dosages: 1 mg/kg
  • Administration: intraperitoneal injection

Solubility (25°C)

In vitro

DMSO 5 mg/mL warmed
(14.02 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
0.5% hydroxyethyl cellulose
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 356.42
Formula

C22H20N4O

CAS No. 545380-34-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)OC2=CC=C(C=C2)CCNC3=NC=NC4=C3C=C(C=C4)N

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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