APX-3330

Catalog No.S7445 Synonyms: E3330

For research use only.

APX-3330 (E3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.

APX-3330 Chemical Structure

CAS No. 136164-66-4

Selleck's APX-3330 has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description APX-3330 (E3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.
Targets
NF-κB [3] APE1(Ref-1) [1]
In vitro

E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. [1] E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. [2] In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hey-C2 cells MnPjSpVv[3Srb36gZZN{[Xl? MofyTY5pcWKrdHnvckBw\iCDcHWxM5Jm\i1zIILl[I95KGGldHn2bZR6KGmwIIDy[ZNmdmOnIH;mJFAvODJibV2gSHRVKGGwZDDoeY1idiCKZYmtR|Ih[2WubIOgcpVkdGWjcjDlfJRz[WO2czDifUBGVVODLDDJR|UxRTFyzszNMi=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB4N{K5NUc,OjByNkeyPVE9N2F-
Hey-C2 cells M4f0eWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkTjO|IhcHK| M4TvXGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmgS2FMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCJSUWwQVM2|ryPLh?= MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNkeyPVEoRjJyME[3NlkyRC:jPh?=
In vivo In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. [4] In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine. [5]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: PANC1 cells
  • Concentrations: ~30 μM
  • Incubation Time: 72 hours
  • Method: PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.
Animal Research:[4]
  • Animal Models: Mice with endotoxin-mediated hepatitis
  • Dosages: 300 mg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 378.46
Formula

C21H30O6

CAS No. 136164-66-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCCCCCC(=CC1=C(C(=O)C(=C(C1=O)OC)OC)C)C(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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