For research use only.

Catalog No.S7445 Synonyms: APX-3330

1 publication

E3330 Chemical Structure

CAS No. 136164-66-4

E3330 (APX-3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.

Selleck's E3330 has been cited by 1 publication

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Biological Activity

Description E3330 (APX-3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.
NF-κB [3]
APE1(Ref-1) [1]
In vitro

E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. [1] E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. [2] In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hey-C2 cells NXPrTY5MTnWwY4Tpc44h[XO|YYm= Ml3wTY5pcWKrdHnvckBw\iCDcHWxM5Jm\i1zIILl[I95KGGldHn2bZR6KGmwIIDy[ZNmdmOnIH;mJFAvODJibV2gSHRVKGGwZDDoeY1idiCKZYmtR|Ih[2WubIOgcpVkdGWjcjDlfJRz[WO2czDifUBGVVODLDDJR|UxRTFyzszNMi=> NFHCVYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC2O|I6OSd-MkCwOlczQTF:L3G+
Hey-C2 cells NHfWelVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NETjXoE4OiCqcoO= NXXjXJA3T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUGW7LVOyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFfJOVA:OzYQvF2u MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB4N{K5NUc,OjByNkeyPVE9N2F-

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In vivo In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. [4] In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine. [5]


Cell Research:[2]
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  • Cell lines: PANC1 cells
  • Concentrations: ~30 μM
  • Incubation Time: 72 hours
  • Method: PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.
    (Only for Reference)
Animal Research:[4]
- Collapse
  • Animal Models: Mice with endotoxin-mediated hepatitis
  • Dosages: 300 mg/kg
  • Administration: p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 75 mg/mL (198.17 mM)
Water Insoluble
Ethanol '75 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 378.46


CAS No. 136164-66-4
Storage powder
in solvent
Synonyms APX-3330

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID