Catalog No.S7445 Synonyms: E3330

For research use only.

APX-3330 (E3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.

APX-3330 Chemical Structure

CAS No. 136164-66-4

Selleck's APX-3330 has been cited by 2 Publications

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Biological Activity

Description APX-3330 (E3330) is a potent and selective APE1(Ref-1) inhibitor, which suppressed NF-kappa B DNA-binding activity.
NF-κB [3] APE1(Ref-1) [1]
In vitro

E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. [1] E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. [2] In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hey-C2 cells MnPjSpVv[3Srb36gZZN{[Xl? MofyTY5pcWKrdHnvckBw\iCDcHWxM5Jm\i1zIILl[I95KGGldHn2bZR6KGmwIIDy[ZNmdmOnIH;mJFAvODJibV2gSHRVKGGwZDDoeY1idiCKZYmtR|Ih[2WubIOgcpVkdGWjcjDlfJRz[WO2czDifUBGVVODLDDJR|UxRTFyzszNMi=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB4N{K5NUc,OjByNkeyPVE9N2F-
Hey-C2 cells M4f0eWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkTjO|IhcHK| M4TvXGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmgS2FMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCJSUWwQVM2|ryPLh?= MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNkeyPVEoRjJyME[3NlkyRC:jPh?=
In vivo In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. [4] In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine. [5]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: PANC1 cells
  • Concentrations: ~30 μM
  • Incubation Time: 72 hours
  • Method: PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.
Animal Research:[4]
  • Animal Models: Mice with endotoxin-mediated hepatitis
  • Dosages: 300 mg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 378.46


CAS No. 136164-66-4
Storage 3 years -20°C powder
2 years -80°C in solvent

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