L-Mimosine

Catalog No.S7446 Synonyms: Leucenol

For research use only.

L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.

L-Mimosine Chemical Structure

CAS No. 500-44-7

Selleck's L-Mimosine has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.
In vitro

L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. [1] L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. [2]

In vivo L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. [3]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: PC-3 and LNCaP cells
  • Concentrations: ~800 μM
  • Incubation Time: 48 hours
  • Method: Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.
Animal Research:[3]
  • Animal Models: Sprague-Dawley rats
  • Dosages: ~50 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 198.18
Formula

C8H10N2O4

CAS No. 500-44-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CN(C=C(C1=O)O)CC(C(=O)O)N

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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