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Spermine

Name: Spermine
Brand: Selleck Chemicals
SKU: S3948
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S3948

Spermine (4,9-Diaza-1,12-dodecanediamine) is a polyamine involved in cellular metabolism found in all eukaryotic cells. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria.

Chemical Structure

Molecular Weight: 202.34

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Quality Control

Batch: Purity: 99.07%

99.07

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Solubility

In vitro
Batch:

DMSO : 40 mg/mL (197.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 40 mg/mL

Ethanol : 40 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.700mg/ml (8.40mM)	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.700mg/ml (8.40mM)	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	202.34	Formula	C₁₀H₂₆N₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	71-44-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	4,9-Diaza-1,12-dodecanediamine			Smiles	C(CCNCCCN)CNCCCN

Mechanism of Action

In vitro	Spermidine and spermine significantly increases the viability and proliferation of SKOV-3 cells in a time-dependent manner, and decreases the apoptotic effect of Difluoromethylornithine (DFMO) in SKOV-3 cells.
In vivo	Pretreatment with SPM significantly alleviated liver injury and reduced intrahepatic inflammation in thioacetamide (TAA)-induced liver injury compared to controls.
References	[1] https://pubmed.ncbi.nlm.nih.gov/34638596/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04339920	Withdrawn	Prostate Cancer	Chinese University of Hong Kong	September 1 2021	--
NCT03914391	Recruiting	Prostate Cancer	Chinese University of Hong Kong	January 16 2019	--
NCT04960956	Terminated	Prostate Cancer\|Urothelial Carcinoma	Indiana University	October 13 2016	--
NCT02855918	Completed	Major Depression	University Hospital Montpellier\|Institut National de la Santé Et de la Recherche Médicale France\|Sys2Diag Mixt laboratory CNRS/Alcediag Montpellier	September 23 2016	Not Applicable

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